A5043109000- Toxin Mechanisms and Immunotoxins
- Natural product bioactivities and synthesis
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Ginseng Biological Effects and Applications
- Phytochemical Studies and Bioactivities
- Plant tissue culture and regeneration
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- PARP inhibition in cancer therapy
- Chromatography in Natural Products
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Pharmacological Effects of Natural Compounds
- Phytoplasmas and Hemiptera pathogens
- Microbial Inactivation Methods
- MicroRNA in disease regulation
- Phytochemistry and Biological Activities
- Phytochemistry and Bioactive Compounds
- Biochemical and Structural Characterization
- Signaling Pathways in Disease
- Advanced biosensing and bioanalysis techniques
- Geochemistry and Elemental Analysis
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
Freie Universität Berlin
2016-2025
Nanyang Technological University
2025
Max Delbrück Center
2022
Charité - Universitätsmedizin Berlin
2011-2017
Great Ormond Street Hospital
2017
University College London
2014-2017
John Carroll University
2013
Vigo County Public Library
2013
Reference Institute for Bioanalytics
2011
University of Copenhagen
2006
While human extracellular vesicles (EVs) have attracted a big deal of interest and been extensively characterized over the last years, plant-derived EVs nanovesicles earned less attention remained poorly investigated. Although series investigations already revealed promising beneficial health effects drug delivery properties, adequate (pre)clinical studies are rare. This fact might be caused by lack sources with appropriate qualities. Our study introduces plant cell suspension culture as new...
Abstract: Saponins are bioactive compounds produced mainly by plants but also some marine organisms and insects. In the recent past, there has been unforeseen interest in clinical utilization of saponins as chemotherapeutic agents. The research on various forms a treatment for cancer generated lot potential. advent nanotechnology cytotoxicity enhancing properties highlights current decade. This review gives an updated overview potential that hold cytotoxic agents, covers literature...
Abstract Statins were shown to possess immunomodulating properties, but the mechanisms of statin effects on immune system are poorly understood. We analyzed influence statins professional antigen-presenting dendritic cells (DC). Immature DC cultivated from monocytes healthy donors. maturation was induced by lipopolysaccharide (LPS; 1 μg/mL). Unstimulated and LPS-stimulated treated with simvastatin or atorvastatin (0.1–1 μM). The expression CD40, CD83, CD86, human leukocyte antigen-DR...
Small extracellular vesicles (sEVs) are heterogeneous biological released by cells under both physiological and pathological conditions. Due to their potential as valuable diagnostic prognostic biomarkers in human blood, there is a pressing need develop effective methods for isolating high-purity sEVs from the complex milieu of blood plasma, which contains abundant plasma proteins lipoproteins. Size exclusion chromatography (SEC) density gradient ultracentrifugation (DGUC) two commonly...
Abstract The inhibition of ENaC may have therapeutic potential in CF airways by reducing sodium hyperabsorption, restoring lung epithelial surface fluid levels, airway hydration and mucociliary function. challenge has been to deliver siRNA the with sufficient efficacy for a sustained effect. We developed self-assembling nanocomplex formulation delivery that consists liposome (DOTMA/DOPE; L), an targeting peptide (P) (R). LPR formulations were assessed their ability silence expression...
Tumor-targeting protein toxins are composed of a toxic enzyme coupled to specific cell binding domain that targets cancer-associated antigens. The anti-tumor treatment by targeted is accompanied dose-limiting side effects. future prospects for therapeutic use in humans will be determined reduce Certain plant secondary metabolites (saponins) were shown increase the efficacy particular epidermal growth factor receptor (EGFR)-targeted toxin, paralleled tremendous decrease This study was...
Abstract Cervical cancer is the second most common in women worldwide. Targeting epidermal growth factor receptor (EGFR) a very promising approach since it overexpressed about 90% of cervical tumors. Here, we quantified toxic effect SE, targeted toxin consisting (EGF) as targeting moiety and plant saporin‐3, on 3 human carcinoma cell lines (HeLa, CaSki SiHa) recently established (PHCC1 PHCC2) from 2 different individuals. A melanocytic mouse line served negative control. Additionally,...
The application of targeted toxins in cancer therapy remains a challenge due to the severe side effects as consequence high systemic doses required. Here, we describe combined glycosylated triterpenoid (Spn) and epidermal growth factor receptor (EGFR)-targeted chimeric (SA2E). cytotoxicity SA2E on murine TSA tumor cells transfected with human EGFR was enhanced 20,000-fold by low nonpermeabilizing Spn concentrations synergistic manner. Subcutaneous BALB/c mice bearing solid resulted 94%...
Targeted toxin‐based therapeutics are hindered by poor intracellular uptake, limited stability and non‐specific immune stimulation. To address these problems, ligand‐targeted toxins in combination with low dose saponin mixtures have been adapted tested vivo the past, however, undefined raw not suitable for use clinical development. In present work we therefore used a targeted toxin (Sap3‐EGF, i.e. saporin fused to epidermal growth factor) structurally defined isolated m/z 1861 (SO‐1861)....
ABSTRACT The epidermal growth factor (EGF) receptor is commonly targeted in cancer therapy because it overexpressed many malignant cells. However, a general problem to couple the targeting moieties and drug molecules way that results homogeneous product. Here, we overcome this issue by engineering variant of EGF with single conjugation site for coupling virtually any payload. recombinant K‐EGF RR was expressed E. coli Rosetta 4–6 mg/L yield. To confirm accessibility introduced functional...
The identification of antiviral natural products as new lead structures has become a major task in medical science. Especially saponins have gained high interest for their activity. Antiviral effects the saponin-containing plant Anagallis arvensis, widely used traditional medicine, been described, while mode action or detailed phytochemical and functional investigations are still missing. A saponin enriched extract (AAS) from aerial material A. arvensis was characterized detail by LC-HRMS,...