A5087110112- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Photonic and Optical Devices
- Advanced Fiber Optic Sensors
- Advanced Biosensing Techniques and Applications
- Acoustic Wave Resonator Technologies
- RNA Interference and Gene Delivery
- Analytical Chemistry and Sensors
- DNA and Nucleic Acid Chemistry
- Plasmonic and Surface Plasmon Research
- Mechanical and Optical Resonators
- Molecular Junctions and Nanostructures
- Microfluidic and Capillary Electrophoresis Applications
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Semiconductor Lasers and Optical Devices
- Electrochemical sensors and biosensors
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- Renal Transplantation Outcomes and Treatments
- Nanoparticle-Based Drug Delivery
- Advanced MEMS and NEMS Technologies
- Advanced Fiber Laser Technologies
- bioluminescence and chemiluminescence research
- Antibiotics Pharmacokinetics and Efficacy
Nello Carrara Institute of Applied Physics
2015-2024
Tehran University of Medical Sciences
2023
National Research Council
2013-2021
University of Florence
2004-2013
Cranfield University
2002-2006
The DNA thrombin aptamer has been extensively investigated, and the coupling of this to different transduction principles demonstrated wide applicability aptamers as bioreceptors in bioanalytical assays. goal work was design an aptamer-based sandwich assay with electrochemical detection for analysis complex matrixes, using a simple target capturing step by aptamer-functionalized magnetic beads. conditions immobilization protein binding have first optimized surface plasmon resonance, then...
Abstract Optical fiber gratings (OFGs), especially long-period (LPGs) and etched or tilted Bragg (FBGs), are playing an increasing role in the chemical biochemical sensing based on measurement of a surface refractive index (RI) change through label-free configuration. In these devices, electric field evanescent wave at fiber/surrounding medium interface changes its optical properties (i.e. intensity wavelength) as result RI variation due to interaction between biological recognition layer...
The advent of optical fiber-based biosensors combined with that nanotechnologies has provided an opportunity for developing in situ, portable, lightweight, versatile, and high-performance sensing platforms. We report on the generation lossy mode resonances by deposition nanometer-thick metal oxide films fibers, which makes it possible to measure precisely accurately changes properties fiber-surrounding medium very high sensitivity compared other technology platforms, such as long period...
Ten genotypes belonging to Lactuca sativa, Cicorium intybus, Plantago coronopus, Eruca and Diplotaxis tenuifolia used in fresh mixed salads were investigated for their polyphenol contents. Flavonoids hydroxycinnamic acids characterized by high-performance liquid chromatography (HPLC)/diode array detection/mass spectrometry. Quercetin, kaempferol, luteolin, apigenin, crysoeriol derivatives identified; all caffeoyl derivatives. The total content was obtained through the Folin−Ciocalteu test...
An evanescent wave optical fiber biosensor based on titania–silica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of sensor, consists a sol–gel-based titania–silica thin film, deposited along sensing portion by means dip-coating technique. Changing both sol viscosity and withdrawal speed during made it possible to adjust thickness film crucial parameter for sensor performance. After functionalization surface using...
Abstract A disposable electrochemical assay involving magnetic particles and carbon‐based screen‐printed electrodes (SPCEs) was developed for the detection of C Reactive Protein (CRP). CRP is a plasma protein among most expressed proteins in acute phase inflammation cases, being known biomarker inflammatory states. The based on sandwich format which RNA aptamer coupled to monoclonal antibody alkaline phosphatase (AP) used as enzymatic label. After assay, modified beads were captured by...
Abstract In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody‐functionalised magnetic beads coupled to screen‐printed cells. The immunoassay based on sandwich format in which primary monoclonal antibody anti‐HER2 protein A modified beads. are then used capture the from sample solution assay performed by adding secondary labelled with biotin. enzyme alkaline phosphatase (AP) conjugated streptavidin its...
A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining best stable conditions during measurements. working range 0.006–70 mg L–1 limit 0.009 were achieved. These results are among compared to other SPR-based biosensors CRP detection, especially considering they...
Label-free and real-time DNA sequence detection in PCR-amplified samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct nonamplified genomic DNA. Here, a piezoelectric biosensor for of sequences is described. The system relies on label-free hybridization reaction between an immobilized probe complementary solution. sensing surface (10 MHz quartz crystals), while present DNA, previously fragmented restriction enzymes.