Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. We report here the isolation characterization oligopeptidase B (OpdB) its corresponding gene from Trypanosoma evansi, a pathogen significant veterinary importance. The T. evansi opdB was present single copy per haploid genome containing an open reading frame 2148 bp encoding protein 80.664 kDa. Purified OpdB hydrolyzed substrates with basic residues P1...

Amiodarone, a commonly used antiarrhythmic, is also potent and selective anti-Trypanosoma cruzi agent. Dronedarone an amiodarone derivative in which the 2,5-diiodophenyl moiety of parental drug has been replaced with unsubstituted phenyl group aiming to eliminate thyroid toxicity frequently observed treatment. approved by Food Drug Administration (FDA), its use as safe antiarrhythmic extensively documented. We show here that dronedarone anti-T. activity, against both extracellular...

This report examines the presence of proteolytic activity detected in media collected from vitro cultures Giardia intestinalis, and partial characterization by gelatin-substrate polyacrylamide gel electrophoresis inhibition studies. Gelatin-substrate revealed 6 bands with activity, estimated molecular weights 36, 59, 63, 72, 103, 175 kDa. These were not present control medium. On other hand, G. intestinalis trophozoite lysates showed at 16, 20, 66, 82, 108, 120 kDa, thus indicating that...

Salivarian trypanosomes use antigenic variation of their variant-specific surface glycoprotein (VSG) coat as a defense against the host immune system. Although about 1000 VSG and pseudo-VSG genes are scattered throughout trypanosome genome, each expresses only one VSG, while rest transcriptionally silent. A 64-kDa glycosylated cross-reacting antigen between Trypanosoma evansi vivax (p64), which was purified from TEVA1 T. Venezuelan isolate, proven here to represent soluble form VSG....

Salivarian trypanosomes sequentially express only one variant surface glycoprotein (VSG) on their cell from a large repertoire of VSG genes. Seven cryopreserved animal trypanosome isolates known as TeAp-ElFrio01, TEVA1 (or TeAp-N/D1), TeGu-N/D1, TeAp-Mantecal01, TeGu-TerecayTrino, TeGu-Terecay03 and TeGu-Terecay323, which had been isolated different hosts identified in several geographical areas Venezuela were expanded using adult albino rats. Soluble forms predominant VSGs expressed during...

Trypanosoma evansi and vivax are the most extensively distributed trypanosomes responsible for diseases in livestock. Western blot indirect immunofluorescence assays revealed a high immunological cross-reaction between these two parasites. An antigen with an apparent molecular mass of 64 kDa (p64), which exhibited cross-reactivity T. , was purified to homogeneity from Venezuelan isolate . This is glycosylated, contains glycosyl-phosphatidylinositol anchor appeared be localized through cell...

Trypanosoma evansi and vivax, which are the major causative agents of animal trypanosomosis in Venezuela, have shown a very high immunological cross-reactivity. Since production T. vivax antigens is limiting factor as this parasite difficult to propagate experimental models, our goal has been identify isolate from that cross-react with vivax. Here, we used Venezuelan TEVA1 prepare total lysate its corresponding cytosolic membranous fractions. In order extract integral membrane proteins,...

Tubulin is the predominant phosphoprotein in Trypanosoma cruzi epimastigotes and phosphorylated by a protein kinase CK2. Interestingly, presence or absence of divalent cations affected solubilization pool parasite tubulin CK2 responsible for its phosphorylation. This fraction co-eluted using phosphocellulose, DEAE-Sepharose Sephacryl S-300 chromatographies. Anti-alpha antibodies co-immunoprecipitated both phosphorylation, anti-CK2 alpha-subunit immunoprecipitated radioactively labelled alpha...

Trypanosoma evansi contains protein kinases capable of phosphorylating endogenous substrates with apparent molecular masses in the range between 20 and 205 kDa. The major phosphopolypeptide band, pp55, was predominantly localized particulate fraction. Anti-alpha anti-beta tubulin monoclonal antibodies recognized pp55 by Western blot analyses, suggesting that this band corresponds to phosphorylated tubulin. Inhibition experiments presence emodin, heparin, 2,3-bisphosphoglycerate indicated...

SUMMARY Trypanosoma evansi and vivax have shown a very high immunological cross-reactivity. Anti- T. antibodies were used to monitor changes in the intracellular Ca 2+ concentration ([Ca ] i ) by fluorometric ratio imaging from single parasites. A short-time exposure of parasites sera -infected bovines induced an increase [Ca , which generated their complete lysis. The parasite boost was reduced but not eliminated absence extracellular or following serum decomplementation. Decomplemented...

Trypanosoma cruzi, its etiological agent, is a flagellate p r o t z n h u d e g s c m l x morphological changes throughout life cycle in both the insect vector and vertebrate host (De Souza, 1984; Andrews et al., 1987).The differentiation process appears to be highly regulated, includes i f b pathways (Urbina 1993), which v environments (Burleigh & Andrews, 1995).Additionally, drastic cytoskeleton of parasite are observed during differentiation.

Abstract A polypeptide band with an apparent molecular weight of 55,000 was phosphorylated in vitro whole-cell lysates Trypanosoma equiperdum . This corresponds to tubulin as demonstrated by immunoprecipitation the from T. extracts when anti-α and anti-β monoclonal antibodies were employed. parasite protein kinase CK2 charge modifying given that common mammalian inhibitors such emodin GTP, hindered phosphorylation exogenously added casein. Interestingly, a divalent cation-dependent...

Salivarian trypanosomes evade the host immune system by continually swapping their protective variant surface glycoprotein (VSG) coat. Given that VSGs from various trypanosome stocks exhibited cross-reactivity (Camargo et al., Vet. Parasitol. 207, 17–33, 2015), we analyzed here which components are antigenic determinants for this cross-reaction. Soluble forms of were purified four Venezuelan animal isolates: TeAp-N/D1, TeAp-ElFrio01, TeAp-Mantecal01, and TeGu-Terecay323. By using VSG soluble...

Los anticuerpos antinucleares (ANA) y los contra antígenos extraíbles del núcleo (ENA) son biomarcadores para el diagnóstico de trastornos autoinmunes, como Lupus Eritematoso Sistémico (LES). Este estudio evaluó patrones ANA asociados a ENA marcadores confirmatorios LES. Se analizaron datos pacientes con sospecha clínica LES, remitidos al laboratorio Santa Rita en Manta, Ecuador, enero diciembre 2020. A estos se les detectaron por inmunofluorescencia sobre células HEp-2 frente inmunoensayo...

Two horses were infected with distinct non-tsetse transmitted Trypanozoon Venezuelan stocks, namely TeAp-N/D1 Trypanosoma equiperdum and TeAp-El Frio01 evansi. Preceding reports have revealed that a 64-kDa antigenic glycopolypeptide (p64), which is the soluble form of predominant variant surface glycoprotein from T. equiperdum, can be used as good antigen for immunodiagnosis animal trypanosomosis. Here, course experimental acute infection in both was monitored by evaluating total anti-p64...

Helicobacter pylori es una bacteria gramnegativa que habita en la mucosa gástrica humana y está asociada con enfermedades como gastritis el cáncer gástrico. El objetivo fue determinar mediante prueba de ureasa, prevalencia H. su asociación patologías gástricas pacientes 25 a 50 años atendidos Instituto Ecuatoriano Enfermedades Digestivas Portoviejo, Ecuador durante año 2018. Las asociaciones entre infección presencia del aparato digestivo; las relaciones edad, sexo por se evaluó estadístico...