A5069139992- Birth, Development, and Health
- Prenatal Substance Exposure Effects
- Reproductive Biology and Fertility
- Pregnancy and preeclampsia studies
- Sperm and Testicular Function
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Nitric Oxide and Endothelin Effects
- Gestational Diabetes Research and Management
- Fatty Acid Research and Health
- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Respiratory Health Research
- Alcohol Consumption and Health Effects
- Viral Infections and Vectors
- Hypothalamic control of reproductive hormones
- Tissue Engineering and Regenerative Medicine
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Assisted Reproductive Technology and Twin Pregnancy
- Neonatal and fetal brain pathology
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Genetics, Aging, and Longevity in Model Organisms
Experimental Medicine and Biology Institute
2017-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
University of Buenos Aires
2016-2025
Fundación Ciencias Exactas y Naturales
2013-2024
Instituto de Biodiversidad y Biología Experimental y Aplicada
2017-2024
Institute of Astronomy and Space Physics
2019-2022
University of California System
2017
University of Chile
2000-2007
Hospital San Borja Arriarán
2000-2001
Centro de Estudios Fotosintéticos y Bioquímicos
1999
It has previously been shown that alcohol can suppress reproduction in humans, monkeys, and small rodents by inhibiting release of luteinizing hormone (LH). The principal action is via suppression the LH-releasing (LHRH) both vivo vitro. present experiments were designed to determine mechanism which inhibits LHRH release. Previous research indicated controlled nitric oxide (NO). proposed pathway norepinephrine-induced NO from NOergic neurons, then activates In experiments, we further...
During development, the remodelling of fibrillar components uterine extracellular matrix (ECM), mediated by metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), plays an essential role in embryonic survival. Previously, we observed that plains viscacha (Lagostomus maximus), only caudal implantation sites (IS) contain viable embryos, whereas embryos at cranial middle IS die are reabsorbed. The objective this study was to analyse distribution expression key endometrial ECM, including...
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects reactive oxygen species (ROS) on mouse preimplantation development, depending the ROS-concentration and time exposition, were studied. Two-cell incubated 5, 10, 25 50 μM hydrogen peroxide (H2O2) for 30 60 minutes exposition allowed develop 72 h study quality development. incubation H2O2 minutes, strongly inhibited 2-cell embryo...
Abstract Chronic arsenic exposure is associated with increased morbidity and mortality for cardiovascular diseases. Arsenic increases myocardial infarction in young adulthood, suggesting that during foetal life correlates cardiac alterations emerging later. Here, we investigated the mechanisms of trioxide (ATO) cardiomyocytes disruption their differentiation from mouse embryonic stem cells. Throughout 15 days presence ATO (0.1, 0.5, 1.0 μM) analysed: expression i) marker genes mesoderm (day...
In infection bacterial products such as lipopolysaccharides (LPS) induce inducible nitric oxide synthase (iNOS) that produces large quantities of NO toxic to the invading organisms, but also often has effects on host cells. Therefore, inhibition iNOS activity might be beneficial in combatting these adverse effects. To determine if methylene blue (MB), an oxidizing agent inactivates iNOS, would reduce levels medial basal hypothalami (MBH) conscious male rats, LPS (5 mg/kg) was injected...
<i>Background/Objective: </i>Nutritional dwarfing (ND) consists of a decrease in weight and height gain delayed onset puberty. The aim the present investigation was to study modifications induced male rats by nutritional stress mere 20% reduction food intake which, however, started immediately after weaning. <i>Materials Methods:</i> At weaning, Wistar were divided into two groups: Control (C) ND. C fed ad libitum with balanced rodent diet. ND received 80% diet...
Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth invasiveness after perigestational 10% ingestion for 15 days preceding up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control [CF] with water). In TF-D4, expanded hatched blastocyst numbers were significantly reduced (p < 0.05) versus CF-D4. Abnormal embryos percentage pyknotic...
Abstract The aim was to study the control females (CF)‐1 mouse embryo differentiation, growth, morphology on embryonic E‐ and N‐cadherin expression at midgestation after periconceptional moderate alcohol ingestion. Adult female mice were exposed 10% ethanol in drinking water for 17 days previous up day 10 of gestation (ethanol‐exposed females, EF) compared with nonexposed CF. EF presented reduced quantities E10 E10.5 embryos, greater percentage embryos stages less than E7.5, implantation...
The zonary and endotheliochorial dog placenta is the most invasive of carnivores. importance matrix metalloproteinases (MMP) in invasiveness has been determined several mammals including species with haemochorial, epitheliochorial placentation. Regarding latter, expression MMP enzymes studied cat mature canine placenta. aim this study was to analyse activity MMP-2 MMP-9 early Placentae from 18 30 days pregnancy were collected four bitches. Two placentae each bitch analysed. Placental tissue...
Perigestational alcohol consumption by CF‐1 mouse, from before mating up to the period of embryo organogenesis, leads retarded early development and neural tube defects. Here, we addressed if perigestational ingestion Day 10 pregnancy induces oxidative stress changes in macromolecules organ tissues organogenic embryos. Adult female mice were administered 10% ethanol their drinking water for 17 days prior until gestation, whereas control females ethanol‐free water. Our results demonstrated...
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic intake on sperm morphology, capacitation, fertilization head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control received ethanol-free water. Similar patterns tyrosine phosphorylation observed capacitated spermatozoa treated males. Percentage hyperactivation (H) spontaneous (SAR) progesterone-induced (IAR) acrosome reaction...
Abstract Background Gestation alcohol consumption produces fetal growth restriction and malformations by affecting the embryo–fetal development. Recently a relationship between abnormal placentation malformation intrauterine retardation has been suggested. However, effects of perigestational ingestion up to early pregnancy on placenta at term its association with abnormalities are little known. Methods In female mice, ethanol 10% in water was administered for 15 days previous 4 (D4), 8 (D8),...
Citation Fontana VA, Sanchez M, Cebral E, Calvo JC. Interferon‐γ inhibits metalloproteinase activity and cytotrophoblast cell migration. Am J Reprod Immunol 2010; 64: 20–26 Problem Establishment of a successful pregnancy relies on complex fetal–mother communication that starts with the embryo adhering invading endometrium. This requires remodeling extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon‐γ (IFN‐γ), play role in implantation could affect success...
Little is known about the effects of low chronic alcohol intake on fertility, particularly in females. Recently, we have shown that 10% (w/v) ethanol treatment affects in-vitro fertilization mouse female gamete. The aim this study was to solve questions concerning lowest dose and duration required alter fertility immature adult male mouse. Mice were treated with 5% 2.5% drinking water for 4 weeks. rates significantly decreased when oocytes from prepubertal pubertal ethanol-treated females...
Long-term changes of different types neurofilaments (NF) and glial fibrilar acid protein (GFAP) were studied in neostriatal rat subjected to perinatal asphyxia (PA) under normothermic hypothermic (15°C) conditions, using immunohistochemistry for light electron microscopy. Neostriatal neurons 6-month-old rats that 19 20 min PA, showed an increase NF 200 kDa immunostaining mainly the axon fascicles comparison with control hypothermia groups. In contrast, no alterations seen NF68 NF160...