A5103134880- Fungal Biology and Applications
- Fish Ecology and Management Studies
- Plant Pathogens and Fungal Diseases
- Aquatic Invertebrate Ecology and Behavior
- Phytochemicals and Antioxidant Activities
- Plant Gene Expression Analysis
- Silymarin and Mushroom Poisoning
- Bacterial Identification and Susceptibility Testing
- Plant biochemistry and biosynthesis
- Freshwater macroinvertebrate diversity and ecology
- Blood Coagulation and Thrombosis Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Mycorrhizal Fungi and Plant Interactions
- Tea Polyphenols and Effects
- Muscle Physiology and Disorders
- Metabolism and Genetic Disorders
- Molecular Communication and Nanonetworks
- Bioactive natural compounds
- Probiotics and Fermented Foods
- Advanced Biosensing Techniques and Applications
- Plant and Fungal Interactions Research
- Salivary Gland Disorders and Functions
- Protein Hydrolysis and Bioactive Peptides
- Plant Pathogens and Resistance
- Reproductive tract infections research
Tohoku University
2018-2024
Akita University
2021
Rakuno Gakuen University
2015-2021
Kyiv City Clinical Oncology Center
2019
RIKEN BioResource Research Center
2016
Abstract Land plants produce diverse flavonoids for growth, survival, and reproduction. Chalcone synthase is the first committed enzyme of flavonoid biosynthetic pathway catalyzes production 2′,4,4′,6′-tetrahydroxychalcone (THC). However, it also produces other polyketides, including p -coumaroyltriacetic acid lactone (CTAL), because derailment chalcone-producing pathway. This promiscuity CHS catalysis adversely affects efficiency biosynthesis, although believed to have led evolution...
Near-infrared (NIR) fluorescent imaging is a powerful tool for the non-invasive visualization of inner structure living organisms. Recently, NIR fluorescence at 1000–1400 nm (second optical window) has been shown to offer better spatial resolution compared with conventional 700–900 (first window). Here we report lead sulfide (PbS) quantum dots (QDs) and their use in vivo cerebral venous thrombosis septic mice. Highly PbS QDs 1100 emission peak (QD1100) were prepared from acetate...
Abstract The plasma concentration and liver mRNA content of IGF-I are regulated by the quantity quality dietary proteins. To determine whether synthesis IGF-binding proteins (BPs) is also affected protein nutrition, we assessed concentration, tissue transcription rate each BP after rats were fed either a 12% casein or protein-free diet for 1 week. Protein deprivation reduced IGFBP-3 IGFBP-4 increased that IGFBP-1 IGFBP-2. in tissues rates did not change response to although their...
Near-infrared (NIR) emitting fluorophores are widely used as bio-imaging probes for non-invasive visualization of deep-tissues at the whole body level, because high permeability and low absorption NIR light in living tissues. Recently much attention has been paid fluorescence imaging wavelengths 1000–1400 nm (second optical window) to obtain clearer deep-tissue images with signal background ratios. This is that longer (1000–1400 nm) highly permeable lower scattering compared conventional...
1. The present paper gives the results of morphological and physiological studies on causal fungus (Cercospora sp.) a new disease tomato.2. symptoms closely resemble “leaf mould tomato (Cladosporium fulvum), ” it is almost impossible to find difference between these two diseases except under microscope.3. widely distributed in Japan, causes often serious damage tomato.4. identical Cercospora diffusa, ecxepting that former has septated conidiophores.5. author proposes common name “Cercospora...
It is known that lactic acid bacteria induce the IL-12. The IL-12 activates NK cells and promotes production of IFN-γ. IFN-γ macrophages resulting in enhanced phagocytosis bactericidal activity. We have been investigating fermented foods activate immune function. In this study, we investigated inducibility using specific antibody. Fermented soybean such as Tempeh Natto are attracting attention terms nutrition, functionality, food problems. induced 1,080 µg/ml IL-12, associated with induction...
Mushrooms are a popular food with well-known functional properties, including immunoregulatory, antitumor, and antibacterial activities. In this study, we screened the anti-inflammatory antimicrobial activities of 44 wild mushrooms growing on campus Rakuno Gakuen University in Japan from September to October 2013. The mushroom species were identified by morphological analysis, rDNA sequencing, and/or protein profiling. Hot-airdried powder samples extracted water, 50% ethanol, or hexane....
Automated rRNA intergenic spacer analysis (ARISA), a method of microbiome analysis, was evaluated for species identification mushrooms based on the specific fragment sizes. We used 51 wild mushroom-fruiting bodies collected in centre Hokkaido and two cultivated mushrooms. Samples were hot-air-dried DNA extracted by beads beating procedure. Sequencing portions gene (rDNA) provided 33 identifications genus or species. The results ARISA combination sizes corresponding to inter regions (ITS2...
Small cladocerans, found abundantly on surfaces of macrophytes and sediments in the littoral zone, are important prey not only for small fish but also various invertebrates such as larvae odonates freshwater habitats. However, no study has documented how habitat substrates affect their behavior vulnerability to predators predation. We conducted laboratory experiments examine movement 3 cladoceran species, Chydorus sphaericus, Alona sp., Ilyocryptus spinifer, determine if vulnerabilities...
Catalytic promiscuity of enzymes plays a pivotal role in driving the evolution plant specialized metabolism. Chalcone synthase (CHS) catalyzes production 2',4,4',6'-tetrahydroxychalcone (THC), common precursor flavonoids, from p-coumaroyl-coenzyme A (-CoA) and three malonyl-CoA molecules. CHS has promiscuous product specificity, producing significant amount p-coumaroyltriacetic lactone (CTAL) vitro. However, mechanistic aspects this remain to be clarified. Here, we show that specificity...
酪農学園大学キャンパス内の自生キノコ48試料について遺伝子解析による同定を行ったところ46試料が種あるいは属レベルで同定された．Congo Red法によるβ-グルカンの定量結果は，測定値にセルロースなどの直鎖状β-グルカンを含まないことから，酵素法と比べて小さい値となったがβ-グルカンの高次構造を反映した機能性を評価する上ではむしろ有用であると考えられた．また食材の機能性評価に汎用されるTPPおよびDPPHラジカル消去活性を測定したところ，両測定値間には強い正の相関が認められ，キノコの抗酸化性にはポリフェノールが関与していることが示唆された．β-グルカン，TPPおよびDPPHラジカル消去活性のいずれの機能性指標においても科ごとに一定の傾向は見られず，種ごとに特徴的な値を示すことが確認された．
It is known to be that lactic acid bacteria induce the IL-12. IL-12 activates NK cells and promotes production of IFN-γ. IFN-γ macrophages, resulting in enhanced phagocytosis bactericidal activity. We have been investigating fermented foods activate immune function. For purpose, a specific antibody required. tried express IL-12p35 by usual method, but was not expressed at all. In present study, we constructed, purified human obtained against IL-12p35. also established method for expressing...
Abstract Because littoral cladocerans are common prey for odonate larvae, they ideal organisms to examine and predator interactions in aquatic habitats. Although death‐feigning behaviour observed is viewed as a trait reduce predation risk, no study has examined the effectiveness of at reducing mortality. We studied by two cladocerans, Chydorus sphaericus Oxyurella tenuicaudis , against larvae Sympetrum frequens . first how detected found that consumed active even under dark conditions, but...
Light with near infra-red wavelength can pass through tissues in a body because of less light absorption and scattering. Especially, near-infrared fluorescence second optical windows (900-1400nm) is expected to be better for deep-tissue vivo imaging by low autofluorescence. Quantum dot (QD) nano-particle which functions as semiconductor, emit near-infra red emission higher signal noise ratio than other fluorescent chemical probes visible region. We previously developed QD from lead sulfate...
Near-infrared (NIR) emitting fluorophores are widely used as bio-imaging probes for non-invasive visualization of deep-tissues at the whole body level, because high permeability and low absorption NIR light in living tissues. Recently much attention has been paid fluorescence imaging wavelengths 1000-1400 nm (second optical window) to obtain clearer deep-tissue images with signal background ratios. This is that longer (1000-1400 nm) highly permeable lower scattering compared conventional...
White bamboo is the swollen stem of Zizania latifolia parasitized by smut fungus Ustilago esculenta. Five samples galls were harvested at different stages swelling, along with young seedlings Z. and isolated colonies U. The inhibition capacity boiling water or 50% ethanol extracts on NO release RAW264 cells, which was stimulated lipopolysaccharide, evaluated as anti-inflammatory activity, while induction immunostimulatory activity. Total polyphenol (TPP) 1,1-diphenyl-2-picrylhydrazyl (DPPH)...
Abstract Die Belichtung von Morusin (I) ergibt in Trichlormethan oder Benzol hoher Ausbeute das Hydroperoxid (IIa); In Methanol, Ethanol tert.‐Butanol reagiert nicht.