A5101903276- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Plant-Derived Bioactive Compounds
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Polyamine Metabolism and Applications
- Sesame and Sesamin Research
- Enzyme Catalysis and Immobilization
- Fungal and yeast genetics research
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Fuel Cells and Related Materials
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Electrochemical sensors and biosensors
- biodegradable polymer synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Fermentation and Sensory Analysis
- Biochemical and biochemical processes
- GABA and Rice Research
- Garlic and Onion Studies
- Cell death mechanisms and regulation
Kanazawa University
2011-2025
Tokyo Gas (Japan)
2011-2023
Tokai National Higher Education and Research System
2021
Gifu University
2021
Tokyo Institute of Technology
2020
Prefectural University of Hiroshima
2017-2019
Tohoku University
2004-2018
NTL Institute for Applied Behavioral Science
2017
Saga University
2015
Candy’s Place
2014
Summary A novel gene, IRE1 , of Saccharomyces cerevisiae was cloned through genetic complementation a myo‐ inositol auxotrophic mutant. The predicted amino acid sequence indicated that encodes protein 126983 Da with two highly hydrophobic regions, probably signal and membrane‐spanning region. carboxy‐terminal region showed close similarity to the catalytic domains kinases. Disruption locus caused myo‐inositol auxotrophy. product is very likely kinase required for synthesis.
Natural rubber (NR) is stored in latex as particles (RPs), molecules surrounded by a lipid monolayer. Rubber transferase (RTase), the enzyme responsible for NR biosynthesis, believed to be member of cis-prenyltransferase (cPT) family. However, none recombinant cPTs have shown RTase activity independently. We show that HRT1, cPT from Heveabrasiliensis, exhibits distinct vitro only when it introduced on detergent-washed HeveaRPs (WRPs) cell-free translation-coupled system. Using this system,...
Abstract Land plants produce diverse flavonoids for growth, survival, and reproduction. Chalcone synthase is the first committed enzyme of flavonoid biosynthetic pathway catalyzes production 2′,4,4′,6′-tetrahydroxychalcone (THC). However, it also produces other polyketides, including p -coumaroyltriacetic acid lactone (CTAL), because derailment chalcone-producing pathway. This promiscuity CHS catalysis adversely affects efficiency biosynthesis, although believed to have led evolution...
Flavonoid metabolons (weakly-bound multi-enzyme complexes of flavonoid enzymes) are believed to occur in diverse plant species. However, how enzymes organized form a metabolon is unknown for most We analyzed the physical interaction partnerships from two lamiales plants (snapdragon and torenia) that produce flavones anthocyanins. In snapdragon, protein-protein assays using yeast systems revealed following binary interactions: flavone synthase II (FNSII)/chalcone (CHS); FNSII/chalcone...
Isoflavone conjugates [7-O-β-D-glucosides and 7-O-(6″-malonyl-β-D-glucosides) of daidzein genistein] accumulate in soybean roots serve as the stored precursors isoflavones (aglycons), which play very important roles rhizobia-mediated nodulation this plant. Thus far, isoflavone 7-O-glucosyltransferase (GmIF7GT or GmUGT1) has been biochemically characterized is believed to be involved conjugate biosynthesis. The genome encodes many other glycosyltransferase homologs (GmUGTs) that are related...
Phosphatidate phosphatase [EC 3.1.3.4] was purified 15- to 20-fold from the soluble fraction of rat liver. The purification procedure involved calcium phosphate gel adsorption and elution, ammonium sulfate precipitation, molecular-sieve chroma-tography. For enzyme assay, an aqueous dispersion phosphatidate, rather than “ membrane-bound ” used as substrate. partially depends almost entirely on presence Mg2+ for its activity. Moreover, activity is stimulated by phosphatidyl-choline. exhibits a...
The carbon backbones of Z,E ‐mixed isoprenoids are synthesized by sequential cis ‐condensation isopentenyl diphosphate (IPP) and an allylic through actions a series enzymes called ‐prenyltransferases. Recent molecular analyses Micrococcus luteus B‐P 26 undecaprenyl (UPP, C 55 ) synthase [Fujihashi M, Zhang Y‐W, Higuchi Y, Li X‐Y, Koyama T & Miki K (2001) Proc Natl Acad Sci USA 98 , 4337–4342.] showed that not only the primary structure but also crystal ‐prenyltransferases were totally...
Using FMN and a reducing agent such as NAD(P)H, type 2 isopentenyl-diphosphate isomerase catalyzes isomerization between isopentenyl diphosphate dimethylallyl diphosphate, both of which are elemental units for the biosynthesis highly diverse isoprenoid compounds. Although flavin cofactor is expected to be integrally involved in catalysis, its exact role remains controversial. Here we report crystal structures substrate-free complex forms from thermoacidophilic archaeon Sulfolobus shibatae,...
A precise mechanism for the biosynthesis of natural rubber has not yet been elucidated. The cis-prenyltransferase (cPT), HRT2, identified from latex Hevea brasiliensis, is thought to be a key enzyme in rubber. This due observation that recombinant expressed Escherichia coli, significantly activated presence centrifuged fraction, resulting formation polyisoprenes corresponding enzymatic characterization cPT function however, investigated because HRT2 E. coli does exhibit significant activity...
The alpha/beta-hydrolases are a family of acid-base-nucleophile catalytic triad enzymes with common fold, but using wide variety substrates, having different pH optima, catalyzing unique reactions and often showing improved chemical thermo stability. ABH prime targets for protein engineering. Here, we have classified active sites from 51 representative members 40 structural fold families into eight distinct conserved geometries. We demonstrate the occurrence motif, acid zone, at turn. show...
Phenol is produced from dietary L-tyrosine by the action of tyrosine phenol-lyase (TPL) gut bacteria and contributes to various physiological disorders, including skin diseases, certain cancers, kidney dysfunction. We found that oral supplementation sesamol (36 or 180 μg mL-1) ad libitum for 14 days in mice significantly suppressed fecal phenol production. Fecal microbiota structure analysis sesamol-supplemented control groups revealed their overall structures were indistinguishable. To...
1-Acylglycerolphosphate acyltransferase (Ec 2.3.1-) and 1-acylglycerolphosphorylcholine (EC 2.3.1.23) of rat liver microsomes were separated from each other. The separation was achieved by sucrose density gradient centrifugation the enzyme preparation that obtained solubilizing with a nonionic detergent, Triton X-100, subjecting solubilized to molecular-sieve chromatography. two acyltransferases are distinguishable other also respect their stabilities heat X-100. Hence, it is concluded these...
Natural rubber of the Para tree (Hevea brasiliensis) is synthesized as a result prenyltransferase activity. The proteins HRT1, HRT2, and HRBP have been identified candidate components biosynthetic machinery. To clarify contribution these to activity, we established cell-free translation system for nanodisc-based protein reconstitution measured enzyme activity protein-nanodisc complexes. Co-expression HRT1 in presence nanodiscs yielded marked polyisoprene synthesis By contrast, neither or...
A human choline kinase cDNA was cloned by complementation of the yeast mutation, cki , from a glioblastoma expression library. The deduced sequence enzyme comprised 456 amino acids with calculated relative molecular mass 52.065. resembled rat liver over entire sequence. It also in carboxy‐terminal region, but not much amino‐terminal region.
Although isopentenyl diphosphate-dimethylallyl diphosphate isomerase is thought to be essential for archaea because they use the mevalonate pathway, its corresponding activity has not been detected in any archaea. A novel type of enzyme, which no sequence similarity known, well-studied enzymes, was recently reported some bacterial strains. In this study, we describe cloning a gene homologue from thermoacidophilic archaeon Sulfolobus shibatae. The heterologously expressed Escherichia coli,...
Natural rubber (NR) is synthesized by the transferase (RTase) on particles (RPs) in latex. Due to heterogeneity of RPs latex, it difficult precisely characterize RTase activity. In this study, we separated Hevea brasiliensis with different particle size distributions, via stepwise centrifugations. Analyses protein compositions and distributions NR suggest that latex can be categorized into two distinct subclasses, larger (termed 1kRP, 2kRP, 8kRP) smaller 20kRP 50kRP). Precise enzymatic...
ABSTRACT Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and droplets are limited. We have succeeded in establishing a system which artificially prepared monolayer added cell-free translation confirm the properties that bind translation-coupled manner.
ABSTRACT cis -polyprenyl diphosphate synthases are involved in the biosynthesis of glycosyl carrier lipid most organisms. However, only little is known about this enzyme archaea. In report, we isolated gene synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius , and characterized recombinant enzyme.
Abstract A novel ruthenium sensitizer TUS-25 with a hexylthiophene-functionalized terpyridine ligand has been synthesized to improve the light-absorption ability of terpyridine-based sensitizers. The incident photon-to-current conversion efficiency DSC at around 400 nm was increased greatly by introduction two hexylthiophene units. showed 7.5% under AM 1.5 (100 mW cm−2) irradiation.