A5010040751- Plant biochemistry and biosynthesis
- Plant-Derived Bioactive Compounds
- Plant Gene Expression Analysis
- Microbial Natural Products and Biosynthesis
- Alcohol Consumption and Health Effects
- biodegradable polymer synthesis and properties
- Lipid metabolism and biosynthesis
- Microbial metabolism and enzyme function
- Gut microbiota and health
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Cassava research and cyanide
- Electron Spin Resonance Studies
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Metal-Catalyzed Oxygenation Mechanisms
- Sesame and Sesamin Research
- Nitrogen and Sulfur Effects on Brassica
- Agricultural and Food Sciences
- Drug Transport and Resistance Mechanisms
- Cancer Research and Treatments
- Natural product bioactivities and synthesis
- Research in Cotton Cultivation
Sumitomo Rubber Industries (Japan)
2016-2024
Tohoku University
2006-2016
Tohoku University Hospital
2006
Natural rubber (NR) is stored in latex as particles (RPs), molecules surrounded by a lipid monolayer. Rubber transferase (RTase), the enzyme responsible for NR biosynthesis, believed to be member of cis-prenyltransferase (cPT) family. However, none recombinant cPTs have shown RTase activity independently. We show that HRT1, cPT from Heveabrasiliensis, exhibits distinct vitro only when it introduced on detergent-washed HeveaRPs (WRPs) cell-free translation-coupled system. Using this system,...
Abstract Chronic consumption of excess ethanol increases the risk colorectal cancer. The pathogenesis ethanol-related cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bacteria in colon and rectum convert acetaldehyde (AcH), which carcinogenic. However, effects chronic on human microbiome are poorly understood role microbes proposed AcH-mediated ER-CRC remains elaborated. Here we analyse compare microbiota structures non-alcoholics alcoholics. microbiotas...
The importance of ethanol oxidation by intestinal aerobes and facultative anaerobes under aerobic conditions in the pathogenesis ethanol-related colorectal cancer has been proposed. However, role obligate therein remains to be established, it is still unclear which bacterial species, if any, are most important production and/or elimination carcinogenic acetaldehyde such conditions. This study was undertaken address these issues.More than 500 strains were isolated from faeces Japanese...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance studies on calmodulin: calcium-dependent spectral change of proteolytic fragmentsMitsuhiko Ikura, Toshifumi Hiraoki, Kunio Hikichi, Osamu Minowa, Haruhiko Yamaguchi, Michio Yazawa, and Koichi YagiCite this: Biochemistry 1984, 23, 14, 3124–3128Publication Date (Print):July 3, 1984Publication History Published online1 May 2002Published inissue 3 July 1984https://doi.org/10.1021/bi00309a003RIGHTS & PERMISSIONSArticle...
Natural rubber of the Para tree (Hevea brasiliensis) is synthesized as a result prenyltransferase activity. The proteins HRT1, HRT2, and HRBP have been identified candidate components biosynthetic machinery. To clarify contribution these to activity, we established cell-free translation system for nanodisc-based protein reconstitution measured enzyme activity protein-nanodisc complexes. Co-expression HRT1 in presence nanodiscs yielded marked polyisoprene synthesis By contrast, neither or...
The triglucoside of sesaminol, i.e., 2,6-O-di(β-D-glucopyranosyl)-β-D- glucopyranosylsesaminol (STG), occurs abundantly in sesame seeds and oil cake serves as an inexpensive source for the industrial production anti-oxidant that displays a number bioactivities beneficial to human health. However, STG has been shown be highly resistant action β-glucosidases, part due its branched-chain glycon structure, these circumstances hampered efficient utilization STG. We found strain (KB0549) genus...
Natural rubber (NR) is synthesized by the transferase (RTase) on particles (RPs) in latex. Due to heterogeneity of RPs latex, it difficult precisely characterize RTase activity. In this study, we separated Hevea brasiliensis with different particle size distributions, via stepwise centrifugations. Analyses protein compositions and distributions NR suggest that latex can be categorized into two distinct subclasses, larger (termed 1kRP, 2kRP, 8kRP) smaller 20kRP 50kRP). Precise enzymatic...
ABSTRACT Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and droplets are limited. We have succeeded in establishing a system which artificially prepared monolayer added cell-free translation confirm the properties that bind translation-coupled manner.
Most cis-prenyltransferases (cPTs) use all-trans-oligoprenyl diphosphate, such as (E,E)-farnesyl diphosphate (FPP, C15 ), but scarcely accept dimethylallyl (DMAPP, C5 an allylic primer in consecutive cis-condensations of isopentenyl diphosphate. Consequently, naturally occurring cis-1,4-polyisoprenoids contain a few trans-isoprene units at their ω-end. However, some Solanum plants have distinct cPTs that primarily DMAPP to synthesize all-cis-oligoprenyl diphosphates, neryl (NPP, C10 ). the...
Paclitaxel, an antineoplastic agent used for the treatment of ovarian cancer, is metabolized by cytochrome P450 (CYP)3A4 and CYP2C8 excreted from cells ATP-binding cassette (ABCB1) (multi-drug resistance [MDR1], P-glycoprotein). Expression these proteins regulated pregnane X receptor (PXR). Although there are common genetic polymorphisms in genes encoding proteins, their effect on clinical efficacy paclitaxel unclear. We therefore examined relationship pharmacokinetics 13 patients with...
The biosynthetic pathway of cytosolic isoprenoids bifurcates after farnesyl diphosphate into sesquiterpene and triterpene pathways. "Metabolic switching" has been used to increase content in plants by suppressing the competitive using transgenic technology. To develop "metabolic without technology, we developed a model system "chemical metabolic inhibitors pathway. Arabidopsis that overexpress amorpha-4,11-diene synthase gene were treated with squalestatin, squalene inhibitor, or...
Neryl diphosphate (C
<title>Abstract</title> Natural rubber (NR) is a sustainable biopolymer consisting mainly of <italic>cis</italic>-1,4-polyisoprene. Modifying an NR biosynthetic enzyme promising strategy to bioproduce novel polymers. Here, we have elucidated the mechanism and successfully developed enzymes that synthesise NR-sized polyisoprenes with unnatural substrates. synthesised by <italic>cis</italic>-prenyltransferase (cPT) on particles (RPs), NR-harbouring lipid monolayer membrane organelles. However,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT3β-Methoxycycloartan-27-oic Acid and Other Constituents from the Leaves of Pseudotsuga japonicaReiko Tanaka, Yoshikazu Takaoka, Haruhiko Yamaguchi, Shunyo MatsunagaCite this: J. Nat. Prod. 1993, 56, 10, 1753–1757Publication Date (Print):October 1, 1993Publication History Published online1 July 2004Published inissue 1 October 1993https://pubs.acs.org/doi/10.1021/np50100a014https://doi.org/10.1021/np50100a014research-articleACS PublicationsRequest...
Abstract Prenyltransferases mediate the biosynthesis of various types polyisoprene compound in living organisms. Natural rubber (NR) Para tree ( Hevea brasiliensis ) is synthesized as a result prenyltransferase activity, with proteins HRT1, HRT2, and HRBP having been identified candidate components biosynthetic machinery. To clarify contribution these to we established cell-free translation system for nanodisc-based protein reconstitution measured enzyme activity protein-nanodisc complexes....
Abstract Natural rubber of the Para tree ( Hevea brasiliensis ) is synthesized as a result prenyltransferase activity. The proteins HRT1, HRT2, and HRBP have been identified candidate components biosynthetic machinery. To clarify contribution these to activity, we established cell-free translation system for nanodisc-based protein reconstitution measured enzyme activity protein-nanodisc complexes. Co-expression HRT1 in presence nanodiscs yielded marked polyisoprene synthesis By contrast,...