A5079905766- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Marine and coastal ecosystems
- Diatoms and Algae Research
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Hemoglobin structure and function
- Enzyme Structure and Function
- Lipid metabolism and biosynthesis
- Microbial Community Ecology and Physiology
- Metal-Catalyzed Oxygenation Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Molecular Biology Techniques and Applications
- Photoreceptor and optogenetics research
- Protist diversity and phylogeny
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Ocean Acidification Effects and Responses
- Electron Spin Resonance Studies
- Amino Acid Enzymes and Metabolism
Centre National de la Recherche Scientifique
2015-2024
Bioénergétique et Ingénierie des Protéines
2015-2024
Aix-Marseille Université
2014-2023
Délégation Provence et Corse
2015-2020
Institut Jacques Monod
1997-2017
Sorbonne Université
1998-2017
Université Paris Cité
1993-2017
Institut de Microbiologie de la Méditerranée
2009-2012
Laboratoire de Microbiologie et Génétique Moléculaires
2006
Institut de Biologie Structurale
2003
Abstract Most aquatic photoautotrophs depend on CO2-concentrating mechanisms (CCMs) to maintain productivity at ambient concentrations of CO2, and carbonic anhydrase (CA) plays a key role in these processes. Here we present different lines evidence showing that the protein LCIP63, identified marine diatom Thalassiosira pseudonana, is CA. However, sequence analysis showed it has low identity with any known CA therefore belongs new subclass designate as iota-CA. Moreover, LCIP63 unusually...
Mitochondria play a key role in the life and death of eukaryotic cells, yet full spectrum mitochondrial functions is far from being fully understood, especially photosynthetic organisms. To advance our understanding cell, an extensive proteomic survey Percoll-purified mitochondria metabolically versatile, hydrogen-producing green alga Chlamydomonas reinhardtii was performed. Different fractions purified cells grown under aerobic conditions were analyzed by nano-liquid...
Protein glutathionylation is a redox post-translational modification occurring under oxidative stress conditions and playing major role in cell regulation signaling. This has been mainly studied nonphotosynthetic organisms, whereas much less known photosynthetic organisms despite their important exposure to caused by changes environmental conditions. We report large scale proteomic analysis using biotinylated glutathione streptavidin affinity chromatography that allowed identification of 225...
The rapid growth in silver nanoparticles (AgNPs) commercialization has increased environmental exposure, including aquatic ecosystem. It been reported that the AgNPs have damaging effects on photosynthesis and induce oxidative stress, but toxic mechanism of is still a matter debate. In present study, model higher plant Spirodela polyrhiza, we found affect significantly inhibit Photosystem II (PSII) maximum quantum yield (Fv/Fm) effective (ΦPSII). changes non-photochemical fluorescence...
A complex of five different enzymes: ribose‐phosphate isomerase, phosphoribulokinase, ribulose‐bisphosphate carboxylase/oxygenase, phosphoglycerate kinase and glyceraldehyde‐phosphate dehydrogenase, has been purified from spinach chloroplasts. These enzymes catalyse consecutive reactions the Calvin cycle, which two catalysed by phosphoribulokinase carboxylase/oxygenase are unique to this cycle. The five‐enzyme successive chromatographies on DEAE‐Trisacryl, Sephadex G‐200 hydroxyapatite....
Summary Diatoms are widespread in aquatic ecosystems where they may be limited by the supply of inorganic carbon. Their carbon dioxide‐concentrating mechanisms ( CCM s) involving transporters and carbonic anhydrases CA well known, but contribution a biochemical C 4 metabolism is contentious. The (s) present marine‐centric diatom, Thalassiosira pseudonana , were studied cells exposed to high or low concentrations CO 2 using range approaches. At possessed based on active uptake (70%...
Widely used silver nanoparticles (AgNPs) are readily accessible to biological fluids and then surrounded by proteins. However, interactions between AgNPs proteins poorly understood. Two dehydrogenases, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) malate (MDH), chosen investigate these interactions. Ag bound thiol groups of enzymes significantly decreases the number free thiols available. Dose-dependent inhibition enzyme activities is observed in both Ag+ treatments. Based on...
CP12 is an 8.5-kDa nuclear-encoded chloroplast protein, isolated from higher plants. It forms part of a core complex two dimers phosphoribulokinase (PRK), tetramers glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and CP12. The role in this assembly has not been determined. To address question, we cloned cDNA encoding the mature green alga Chlamydomonas reinhardtii expressed it Escherichia coli. Sequence alignments show that very similar to other CP12s, with four conserved cysteine residues...
Aquatic photosynthesis is responsible for about half of the global production and undertaken by a huge phylogenetic diversity algae that are poorly studied. The redox-regulation phosphoribulokinase (PRK) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was investigated in wide range algal groups under standard conditions. Redox-regulation PRK greatest chlorophytes, low or absent red alga most chromalveolates, linked to number amino acids between two regulatory cysteine residues. GAPDH...
Keeping tabs on tyrosine: A three-component Mannich-type reaction extends the scope of site-directed spin labeling by selectively unique tyrosine residue CP12 protein (see picture), as was confirmed mass spectrometry. EPR spectroscopy labeled showed a very high mobility probe, which remained mobile after complex formation with GAPDH.
Abstract The concentration of CO 2 in many aquatic systems is variable, often lower than the K M primary carboxylating enzyme Rubisco, and order to photosynthesize efficiently, algae operate a facultative concentrating mechanism (CCM). Here we measured responses marine diatom, Thalassiosira pseudonana , high low concentrations at level transcripts, proteins activity. Low caused metabolic pathways be remodeled. Carbon acquisition enzymes, primarily carbonic anhydrase, stress, degradation...
Ottelia alismoides is a constitutive C4 plant and bicarbonate user, has facultative crassulacean acid metabolism (CAM) at low CO2. Acclimation to factorial combination of light CO2 showed that the ratio phosphoenolpyruvate carboxylase (PEPC) ribulose-bisphosphate carboxylase/oxygenase (Rubisco) (>5) in range plants. This short-term response experiments activity PEPC pyruvate phosphate dikinase (PPDK) was high even end night, consistent with night-time accumulation daytime carbon fixation....