A5057184094- Advanced biosensing and bioanalysis techniques
- Dementia and Cognitive Impairment Research
- Alzheimer's disease research and treatments
- Smoking Behavior and Cessation
- Urinary Tract Infections Management
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Genetic Associations and Epidemiology
- Bioinformatics and Genomic Networks
- Antibiotic Use and Resistance
- Data-Driven Disease Surveillance
- Health, Environment, Cognitive Aging
- Knee injuries and reconstruction techniques
- Patient Satisfaction in Healthcare
- Time Series Analysis and Forecasting
- Anomaly Detection Techniques and Applications
- COVID-19 and healthcare impacts
- Total Knee Arthroplasty Outcomes
- Nutrition, Genetics, and Disease
- School Health and Nursing Education
- Antimicrobial Resistance in Staphylococcus
- bioluminescence and chemiluminescence research
- Occupational health in dentistry
- Biotin and Related Studies
Ningxia Medical University
2024
Hospital for Special Surgery
2024
University School
2024
Washington University in St. Louis
2017-2024
Alzheimer’s Disease Neuroimaging Initiative
2023
Saint Louis University
2014-2022
Museum of Heilongjiang Province
2021
Nanjing University
2013-2017
Nanjing General Hospital of Nanjing Military Command
2013-2017
Barnes-Jewish Hospital
2017
Hail CESA! Cascade enzymatic signal amplification (CESA) for DNA detection is achieved in three steps: invasive by Afu endonuclease to generate amplified flaps, flap ligation T4 ligase form a nicking site, and reaction produce signals (see picture last step). The sensitivity of CESA as high 1 fM DNA.
We examined past-12-month quit attempts and smoking cessation from 2006 to 2016 while accounting for demographic shifts in the US population. In addition, we sought understand whether current use of electronic cigarettes was associated with a change successful at population level.We analyzed data 25- 44-year-olds National Health Interview Survey (NHIS) (N = 26,354) Tobacco Use Supplement Current Population (TUS-CPS) 2006-2007, 2010-2011, 2014-2015 33,627). Data on e-cigarette were available...
White matter hyperintensities (WMH) correlate with Alzheimer disease (AD) biomarkers cross-sectionally and modulate AD pathogenesis. Longitudinal changes have been reported for biomarkers, including concentrations of CSF β-amyloid (Aβ) 42, Aβ40, total tau phosphorylated tau181, standardized uptake value ratio from the molecular imaging cerebral fibrillar Aβ PET using [
Abstract Objective: To examine rural–urban differences in temporal trends and risk of inappropriate antibiotic use by agent duration among women with uncomplicated urinary tract infection (UTI). Design: Observational cohort study. Methods: Using the IBM MarketScan Commercial Database (2010–2015), we identified US commercially insured aged 18–44 years coded for UTI prescribed an oral agent. We classified agents durations as appropriate versus based on clinical guidelines. Rural–urban status...
Amyloidosis, including cerebral amyloid angiopathy, and markers of small vessel disease (SVD) vary across dominantly inherited Alzheimer's (DIAD) presenilin-1 (PSEN1) mutation carriers. We investigated how position relative to codon 200 (pre-/postcodon 200) influences these pathologic features dementia at different stages. Individuals from families with known PSEN1 mutations (n = 393) underwent neuroimaging clinical assessments. cross-sectionally evaluated regional Pittsburgh compound...
Abstract Approximately 5% of Alzheimer’s disease cases have an early age at onset (<65 years), with 5–10% these attributed to dominantly inherited mutations and the remainder considered as sporadic. The extent which sporadic early-onset overlap is unknown. In this study, we explored clinical, cognitive biomarker profiles disease, focusing on commonalities distinctions between cases. Our analysis included 117 participants enrolled in Dominantly Inherited Alzheimer Network 118...
7515 Background: In DREAMM-8 (NCT04484623), BPd demonstrated a statistically significant, clinically meaningful benefit to progression-free survival (PFS) vs PVd in pts with RRMM who received ≥1 prior line of treatment including lenalidomide. MRD neg has been shown be predictor PFS and overall (OS) MM. We assessed efficacy outcomes by status. Methods: Ptswere randomized (1:1) or PVd. The primary endpoint was independent review committee (IRC)–assessed PFS; OS, duration response, status...
Detecting DNA biomarkers related to personalized medicine could improve the outcome of drug therapy. However, in a resource-restrained hospital is very difficult because biomarker detection should be performed by well-trained staff and requires expensive laboratory facilities.We developed gold nanoparticle-based "Tube-Lab" enable analysis closed tube. Gold nanoparticle-modified probes (GNPs) were used construct an inexpensive simple sensor for signal readout. The method consists 3 steps...
Little is known about the relative harms of different antibiotic regimens prescribed to treat uncomplicated urinary tract infection (UTI). We sought compare risk adverse events associated with commonly used oral for outpatient treatment UTI.Using data from IBM® MarketScan® Commercial Database, we identified 1 169 033 otherwise healthy, nonpregnant women aged 18-44 years UTI who initiated an activity against common uropathogens July 2006 30 September 2015. propensity score-weighted...
Abstract Background Occupational exposures may play a key role in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection risk. We used job-exposure matrix linked to the UK Biobank measure occupational characteristics and estimate associations with positive SARS-CoV-2 test. Methods People reporting job titles at their baseline interview England who were < 65 years of age 2020 included. Healthcare workers excluded because differential access testing. Jobs US Information...
UK Biobank (UKB) is a large prospective cohort capturing numerous health outcomes, but limited occupational information (job title, self-reported manual work and walking/standing).
To digitally analyze expression levels of multiple genes in one reaction, we proposed a method termed as 'MDHB' (Multiplexed Digital-PCR coupled with Hydrogel Bead-array). The template for bead-based emulsion PCR (emPCR) was prepared by reverse transcription using sequence-tagged primers. beads recovered from emPCR were immobilized hydrogel to form single-bead layer on chip, and then decoded gene-specific probe hybridization Cy3-dUTP based primer extension reaction. specificity improved...
Detection of nucleic acids with signal amplification is preferable in clinical diagnosis. A novel approach was developed for by coupling invasive reaction hyperbranched rolling circle (HRCA). Invasive reaction, which does not rely on specific recognition sequences a target but structure formed the binding an upstream probe and downstream to DNA, can generate thousands flaps from one DNA; then are ligated padlock probes form circles, templates HRCA. As HRCA amplicon sequence free DNA...
Effective smoking cessation medications are readily available but may be underutilized in hospital settings. In our large, tertiary care hospital, we aimed to (1) characterize patient tobacco use prevalence across medical specialties, (2) determine pharmacotherapy prescription variation and (3) identify opportunities for improvement practice. Using electronic health records at Barnes Jewish Hospital, gathered demographic data, admitting service, admission route, length of stay, self-reported...
Purpose Lectins are valuable tools for detecting specific glycans in biological samples, but the interpretation of measurements can be ambiguous due to complexities lectin specificities. Here, we present an approach improve accuracy by converting into quantitative predictions presence various glycan motifs. Experimental design The conversion relies on a database analyzed array data that provides information specificities lectins each We tested method using binding arrays and then applied...
Acute otitis media (AOM) is a common indication for antibiotics in children. We sought to characterize the frequency of nonguideline concordant antibiotic therapy AOM United States, by agent and duration.
Aims The purpose of this study is to determine an individual’s age-specific prevalence total knee arthroplasty (TKA) after cruciate ligament surgery, and identify clinical genetic risk factors associated with undergoing TKA. Methods This was a retrospective case-control using the UK Biobank individuals reporting history surgery. Data from verbal procedural codes recorded through NHS were used instances Patient data for progression surgery Individuals without reconstruction comparison....
The purpose of this study is to examine the predictive utility polygenic risk scores (PRSs) for smoking behaviors.
Objectives Physically-demanding occupations may increase rotator cuff disease (RCD) risk and need for surgery. We linked a job-exposure matrix (JEM) to the UK Biobank cohort study measure physical occupational exposures estimate associations with RCD Methods Jobs Standard Occupational Classification (SOC) codes were recorded during verbal interview. Lifetime job histories captured through web-based survey. SOC JEM based on US O*NET database. O*NET-based scores [static strength, dynamic...
Reducing adverse events from pharmacologic treatment is an important goal of precision medicine and identifying genetic predictors a step toward this goal. In 2012, King et al. reported associations between variants in placebo-controlled smoking cessation trial varenicline bupropion. Strong were found gastrointestinal 11 the CHRNA5-CHRNA3-CHRNB4 region chromosome 15, repeatedly associated with smoking-related phenotypes. Our was to replicate, independent sample, impact on extend analyses...
Empfindliches Diagnosewerkzeug: Eine kaskadische enzymatische Signalverstärkung (CESA) für den DNA-Nachweis wird in drei Stufen erreicht: invasive durch Afu-Endonuklease zur Erzeugung amplifizierter Flap-Sequenzen, Ligation der Flap-Sequenzen T4-Ligase Bildung einer Nicking-Stelle und Nicking-Reaktion die Nicking-Endonuklease (siehe Bild letzten Schritt). CESA erreicht eine Empfindlichkeit von 1 fM DNA.