A5085283811- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Gene expression and cancer classification
- Advanced Biosensing Techniques and Applications
- Protist diversity and phylogeny
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Renal and related cancers
- Identification and Quantification in Food
- MicroRNA in disease regulation
- Computational Drug Discovery Methods
- Cancer Cells and Metastasis
- Genomic variations and chromosomal abnormalities
- Microbial Community Ecology and Physiology
- Genetically Modified Organisms Research
- Colorectal Cancer Screening and Detection
- SARS-CoV-2 detection and testing
- DNA and Biological Computing
China Pharmaceutical University
2007-2025
Southern Medical University
2022-2024
Nanjing University
2013-2022
Nanjing General Hospital of Nanjing Military Command
2013-2022
State Key Laboratory of Analytical Chemistry for Life Science
2016-2022
Nanjing Medical University
2014-2020
Nanjing Library
2014-2020
Jinling Institute of Technology
2015-2020
Huadong Hospital
2009-2012
Tsinghua University
2006
monitoring of the actions correlated enzymes in living cells is crucial for expanding our understanding disease progression and evaluating drug efficacy. However, due to diverse functions different enzymes, currently available methods comprehensive analysis these events are limited. Here, we present an
Hail CESA! Cascade enzymatic signal amplification (CESA) for DNA detection is achieved in three steps: invasive by Afu endonuclease to generate amplified flaps, flap ligation T4 ligase form a nicking site, and reaction produce signals (see picture last step). The sensitivity of CESA as high 1 fM DNA.
Abstract The potentially different genetics and epigenetics in the primary tumors metastases affect efficacy of treatment breast cancer patients. Nevertheless, cellular molecular mechanisms lymph node metastasis still remain elusive. Here, we employed single-cell RNA sequencing to acquire transcriptomic profiles individual cells from tumors, negative nodes (NLs) positive (PLs). We also performed a assay for transposase-accessible chromatin (ATAC) (scATAC-seq) NL samples get accessibility...
DNA walkers have shown superior performance in biosensing due to their programmability design molecular walking behaviors with specific responses different biological targets. However, it is still challenging make capable of distinguishing targets single-base differences, so that can be used for circulating tumor sensing are rarely reported. Herein, a flap endonuclease 1 (FEN 1)-assisted walker has been proposed achieve mutant biosensing. The target captured on gold nanoparticle (AuNP) as...
Abstract To determine the prognostic value of timing circulating breast tumour cell measurement during treatment, peripheral blood from 164 patients with disease was collected. Circulating cells (CTCs) were enriched by using immunomagnetic nanospheres (IMNs) and identified immunofluorescent staining. The CTC shows nuclear-positive, EpCAM-positive, CK19-positive, CD45-negative. Patients positivity (> 19/7.5 mL blood) had shorter progression-free survival (PFS) overall (OS) than those...
In situ observation of changes in the activity marker proteins living cells is crucial for both biomarker-based disease diagnosis and drug screening. Flap endonuclease 1 (FEN1) has been recognized as a broad-spectrum cancer biomarker therapeutic target. However, simple reliable methods studying FEN1 are limited. Here, we introduce nano firework fluorescent sensor to sense report through recognizing substrates on surface release restore fluorescence prequenched fluorophores. We verified high...
This work developed DNA amplifier logic gates (AND-OR, OR-AND, FAN-IN, FAN-OUT, and 4-bit square-root circuits) using a flap endonuclease 1 (FEN1)-catalyzed signal amplification reaction, for the fastest compact computing.
Genetic polymorphism and environment each influence individual variability in drug metabolism disposition. It is preferable to predict such variability, which may affect efficacy toxicity, before administration. We examined differences the pharmacokinetics of atorvastatin by applying gas chromatography-mass spectrometry-based metabolic profiling predose plasma samples from 48 healthy volunteers. determined level using liquid chromatography-tandem mass spectrometry. With endogenous molecules,...
Digital nucleic acid analysis technology has shown great application potential due to its excellent performance. However, most current digital detection methods are based on PCR or other template amplification strategies. Here, we present an alternative platform signal in droplets termed dNASA. Using a bead-based controllable extension bridged cascade reaction, achieved ultralow background, high efficiency, and highly specific analysis. As "proof of concept", demonstrated the feasibility...
Abstract Nucleic acid drugs, which trigger gene silencing by hybridizing with target genes, have shown great potential in targeting those undruggable targets. However, most of the existing nucleic drugs are only sequence specific for genes and lack cellular or tissue selectivity, challenges their therapeutic safety. Here, study proposes a tumor cell‐specific strategy using hairpin DNA oligonucleotides to RNA degrading highly expressed endogenous flap endonuclease 1 (FEN1) cells, selective...
Abstract: Influenza virus infections represent a worldwide public health and economic problem due to the significant morbidity mortality caused by seasonal epidemics pandemics. Sensitive convenient methodologies for detection of influenza viruses are essential further disease control. Loop-mediated isothermal amplification (LAMP) is most commonly used method nucleic acid amplification. However, with regard multiplex LAMP, differentiating ladder-like LAMP products derived from multiple...
Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3′ structure, and cleavage domain Fok I (Fn1), cleaves DNA strands. The SGN target on basis structure formed between guide (gDNA) cut through its Fn1 dimerization. Our results show that SGN, guided by pair gDNAs, transgenic reporter gene endogenous genes in...
In recent years, several approaches have been applied to modify metal–organic frameworks (MOFs) owing their excellent structural tunability such as higher extraction efficiency than that of primitive crystals. Herein, Zr-based MOFs (UiO-66-NH2) with a suitable size modulated by acetic acid were successfully synthesized for effective DNA extraction. The bonding conformations and adsorption mechanism indicated high affinity between UiO-66-NH2 the molecules. Furthermore, Fe3O4 nanoparticles...
Detecting DNA biomarkers related to personalized medicine could improve the outcome of drug therapy. However, in a resource-restrained hospital is very difficult because biomarker detection should be performed by well-trained staff and requires expensive laboratory facilities.We developed gold nanoparticle-based "Tube-Lab" enable analysis closed tube. Gold nanoparticle-modified probes (GNPs) were used construct an inexpensive simple sensor for signal readout. The method consists 3 steps...
An extension-block base in a molecular beacon enables beacon-assisted strand-displacement amplification to couple with invasive reaction efficiently by flap extension.
A lateral flow strip (LFS) is an ideal tool for point-of-care testing (POCT), but traditional LFSs cannot be used multiplex detection. Herein, a and versatile LFS based on flap endonuclease 1 (FEN1)-induced steric hindrance change (FISH-LFS) proposed. In this method, PCR coupled with cascade invasive reactions was employed to yield single-stranded flaps, which were target-specific independent of target sequences. Then, the amplicons applied FISH-LFS, flaps would efficiently captured by...
Liquid biopsy as well genotyping plays important roles in guiding the use of tumor-targeted drugs and monitoring generation drug resistance. However, current methods, such next-generation sequencing (NGS) pyrosequencing, require long analysis time complicated steps. To achieve ultrafast highly specific detection cell-free DNA (cfDNA) from blood, we improved our recently developed FEN1-aided RPA (FARPA), which combined flap endonuclease 1 (FEN1)-catalyzed invasive reactions with recombinase...
LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of amplicons lead to cross-contamination due need open reaction tube which contains a large number amplicons. To closed-tube detection, we have developed separates solution SYBR Green I (SGI) from reagents using temperature-sensitive wax. The SGI sealed in bottom so not interfere with reaction, but released into mixture after completion by melting enable...