N-linked glycosylation is one of the most important post-translational modifications proteins. Current knowledge multicellular eukaryote N-glycan biosynthesis suggests high mannose N-glycans are generated in endoplasmic reticulum and Golgi apparatus through conserved biosynthetic pathways. According to conventional pathways, four Man7GlcNAc2 isomers, three Man6GlcNAc2 Man5GlcNAc2 isomer during this process. In study, we applied our latest mass spectrometry method, logically derived sequence...

Collision-induced dissociation (CID) of sodiated glucose was investigated using electronic structure calculations and resonance excitation in a low-pressure linear ion trap. The major channels addition to desodiation are dehydration C2H4O2 elimination reactions which the barrier heights near or lower than sodiation energy glucose. Dehydration reaction involves transfer H atom from O2 O1 atom, followed by cleavage C1-O1 bond. Notably, α-glucose has that β-glucose. This difference results...

Abstract Carbohydrates have various functions in biological systems. However, the structural analysis of carbohydrates remains challenging. Most commonly used methods involve derivatization or can only identify part structure. Here, we report a de novo method for completely identification underivatised oligosaccharides. This method, which provide assignments linkages, anomeric configurations, and branch locations, entails low-energy collision-induced dissociation (CID) sodium ion adducts...

Collision-induced dissociation of sodiated α-glucose, β-glucose, α-galactose, β-galactose, α-mannose, and β-mannose was studied using electronic structure calculations resonance excitation in a low-pressure linear ion trap. We made an extensive search conformers transition states to ensure the state with lowest barrier height for each channel could be located. The major channels, addition desodiation, are cross-ring dehydration. Cross-ring starts H atom transfer from O1 O0 atom, followed by...

N-linked glycosylation is one of the most important protein post-translational modifications. Despite importance N-glycans, structural determination N-glycan isomers remains challenging. Here we develop a mass spectrometry method, logically derived sequence tandem (LODES/MSn), to determine structures that cannot be determined using conventional spectrometry. In LODES/MSn, sequences successive collision-induced dissociation are from carbohydrate mechanisms and apply N-glycans in an ion trap...

Oligosaccharides have diverse functions in biological systems. However, the structural determination of oligosaccharides remains difficult and has created a bottleneck carbohydrate research. In this study, new approach for de novo underivatized is demonstrated. A low-energy collision-induced dissociation (CID) sodium ion adducts was used to facilitate cleavage desired chemical bonds during dissociation. The selection fragments subsequent CID guided using procedure that we built from...

Abstract Glycans have diverse functions and play vital roles in many biological systems, such as influenza, vaccines, cancer biomarkers. However, full structural identification of glycans remains challenging. The glycan structure was conventionally determined by chemical methods or NMR spectroscopy, which require a large amount sample are not readily applicable for extracted from samples. Although it has high sensitivity is widely used determination molecules, current mass spectrometry can...

Abstract Free oligosaccharides are abundant macronutrients in milk and involved prebiotic functions antiadhesive binding of viruses pathogenic bacteria to colonocytes. Despite the importance these oligosaccharides, structural determination is challenging, oligosaccharide biosynthetic pathways remain unclear. Oligosaccharide structures conventionally determined using a combination chemical reactions, exoglycosidase digestion, nuclear magnetic resonance spectroscopy, mass spectrometry. Most...

N-linked glycosylation is one of the most important post-translational modifications proteins. Current knowledge multicellular eukaryote N-glycan biosynthesis suggests that N-glycans are produced in endoplasmic reticulum (ER) and Golgi apparatus through conserved biosynthetic pathways. According to this biosynthesis, only isomer GlcNAc(Man3GlcNAc2) two isomers GlcNAc2(Man3GlcNAc2) generated, all larger complex generated from these isomers. In study, we applied our latest mass spectrometry...

A series of novel branched-chain glycosides were synthesised from xylose, an aldopentose and Guerbet alcohols whose total number carbon atoms ranges C₈–C₂₄. Interestingly dry <italic>β</italic>-Xyl-C₈C₄ gave a rectangular columnar ribbon phase at 25 °C.

Despite the importance of carbohydrates in biological systems, structural determination remains difficult because large number isomers. In this study, a new mass spectrometry method, namely logically derived sequence tandem (LODES/MSn), was developed to characterize oligosaccharide structures. approach, sequential collision-induced dissociation (CID) oligosaccharides is performed an ion trap spectrometer identify linkage position, anomeric configuration, and stereoisomers each monosaccharide...

Electrospray ionization (ESI) and matrix-assisted laser desorption (MALDI) are soft techniques commonly used in mass spectrometry. Although in-source post-source decays of MALDI have been investigated extensively, the analogous ESI received little attention. Previous studies regarding focus on dissociation multiply charged proteins peptides. The decay carbohydrates has not yet, it may interference carbohydrate structural determination.Commercial apparatus, including a high-performance liquid...

Cubic liquid crystalline nanoparticles (cubosomes) of bicontinuous nature with internal networks water channels have received great interests in nanomedicine applications, particularly as potential vehicle for loading and release therapeutic agents. These been most commonly produced using monoolein phytantriol. In this study, we explore the use a Guerbet branched chain glycolipid, namely 2-hexyl-decyl-β-D glucopyranoside (β-Glc−OC10C6), new alternative material cubosomes production. The...

-Linked glycosylation is one of the most essential post-translational modifications proteins. However,

High mannose

Determining carbohydrate structures, such as their compositions, linkage positions, and in particular the anomers stereoisomers, is a great challenge. Isomers of different or stereoisomers have same sequences chemical bonds, but orientations some bonds which are difficult to be distinguished by mass spectrometry. Collision-induced dissociation (CID) tandem spectroscopy (MS/MS) widely used technique for characterizing structures. Understanding mechanism important obtaining structural...

Collision-induced dissociation (CID) tandem mass spectrometry is commonly used for carbohydrate structural determinations. In the CID approach, carbohydrates are dissociated into fragments, and this followed by identification of fragments through subsequent CID. The success analysis depends on correlation before after dissociation, that is, memory fragments. Fragments completely lose their original structures cannot be analysis. By contrast, with extremely strong correlations between...

Fructooligosaccharides (FOS) and raffinose family oligosaccharides (RFOs) are two highly abundant water-soluble carbohydrates in plants. The typical procedures for the FOS RFO structural determination using mass spectrometry involve permethylation, followed by hydrolysis of permethylated into monosaccharides, then identification linkage positions GC spectrometry. However, position sequence is not straightforward, thus this method limited to small or with simple linkages. In study, we...

Characterization of carbohydrate structures using mass spectrometry is a challenging task. Understanding the dissociation mechanisms carbohydrates in gas phase crucial for characterizing these through tandem spectrometry. In this study, we investigated collision-induced (CID) glucose, galactose, and mannose their linear forms, as well forms hexose at reducing end 1-6 linked disaccharides, quantum chemistry calculations Our results suggest that dehydration reaction unlikely to occur due...

N-linked glycosylation is one of the most important post-translational modifications proteins. Current knowledge multicellular eukaryote N-glycan biosynthesis suggests high mannose N-glycans are generated in endoplasmic reticulum and Golgi apparatus through conserved biosynthetic pathways. As a part modifications, lipid dolichol-phosphate linked oligosaccharide Glc3Man9GlcNAc2 transferred to proteins, glucoses sequentially removed by various ER- Golgi-localized glucosidases...