A5044703855- Drug Solubulity and Delivery Systems
- Surfactants and Colloidal Systems
- Lipid Membrane Structure and Behavior
- Infant Nutrition and Health
- Metabolomics and Mass Spectrometry Studies
- Proteins in Food Systems
- Pharmaceutical studies and practices
- Advanced Drug Delivery Systems
- Drug Transport and Resistance Mechanisms
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Food Chemistry and Fat Analysis
- Microfluidic and Capillary Electrophoresis Applications
- Fatty Acid Research and Health
- Analytical Chemistry and Chromatography
- Crystallization and Solubility Studies
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Liquid Crystal Research Advancements
- Pneumocystis jirovecii pneumonia detection and treatment
- Breastfeeding Practices and Influences
- Antibiotics Pharmacokinetics and Efficacy
- Electrohydrodynamics and Fluid Dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Poisoning and overdose treatments
Monash University
2017-2025
University of Malaya
2014-2022
Hospital Kuala Lumpur
2015
University of Sheffield
2006-2012
National Research Centre
2010
The increasing popularity of iTRAQ for quantitative proteomics applications makes it necessary to evaluate its relevance, accuracy, and precision biological interpretation. Here, we have assessed (a) the accuracy quantification in a controlled experimental setup, using low- high-complexity protein mixtures; (b) potential pitfalls that hamper applicability attainable dynamic range iTRAQ: isotopic contamination, background interference, signal-to-noise ratio. Our data suggest greater crosstalk...
Application of iTRAQ-based workflows for protein profiling has become widespread. Concomitantly, the idiosyncratic limitations iTRAQ, such as its tendency to underestimate quantifications, have been studied and recognised. This report shows that influence ratio compression limiting transmission in iTRAQ MS/MS high-complexity mixtures (iTRAQ-labelled lysates) can be partly alleviated using high-resolution sample fractionation. Here, we also investigate greater detail dependency quantification...
Our review highlights lipid liquid crystal nanocarriers, essentially their design considerations and sugar-based materials for specific targeted delivery.
Amaranth grain is reputed to have a high nutritional value, and as plant, be tolerant adverse weather conditions. This suggests that amaranth could useful in tackling malnutrition the growing burden of cardiometabolic diseases. However, there insufficient knowledge at present about how nutrient composition digestibility varies with environment, crop genotype, post-harvest processing. We investigated effect source processing grains on protein lipid grains. There was variation raw from...
Isobaric tags for absolute and relative quantification (iTRAQ) provide the quantitative proteomics community with an easy-to-use tool to examine proteome changes. Five years after launch of technique, has become familiar iTRAQ? In this review, we propose answer question by looking at two its facets. On one hand, enquire whether iTRAQ is used 'optimally'; in other words, keeping up methods that have been devised requirements recognised, proteomics. there paper written such a way as allow...
Milk has previously been considered as a potential lipid-based drug delivery system for poorly water soluble drugs but it never gained significant attention. This is in part because relying on solubility formulations (in this case milk) does not provide complete picture of the behavior such systems upon digestion. Herein, we demonstrate using time resolved X-ray scattering that digestion milk actually crucial to solubilisation water-soluble drug, halofantrine. Halofantrine was chosen its...
Lipids in mammalian milks such as bovine milk and human breast have been shown to self-assemble into various liquid crystalline materials during digestion. In this study, the direct correlation between composition of lipids from three types milk, brands infant formulas (IFs), soy structures that form their digestion was investigated link material properties composition. The self-assembly behavior assessed using vitro coupled with situ small-angle X-ray scattering (SAXS). Lipid determined ex...
Milk has been used as a vehicle for the delivery of antimalarial drugs during clinical trials to test food effect and artefenomel (OZ439) showed enhanced oral bioavailability with milk. However, nature interaction between milk OZ439 in gastrointestinal tract remains poorly understood. To understand role digestion on solubilization polymorphism, we conducted real-time monitoring crystalline drug suspension vitro intestinal lipolysis containing using synchrotron X-ray scattering. formed an...
Milk is an attractive lipid-based formulation for the delivery of poorly water-soluble drugs to pediatric populations. We recently observed that solubilization artefenomel (OZ439) during in vitro intestinal lipolysis was driven by digestion triglycerides full-cream bovine milk, reflecting ability milk act as enabling clinic. However, when OZ439 co-administered with a second antimalarial drug, ferroquine (FQ) exposure reduced. The current study therefore aimed understand impact presence FQ on...
Abstract Background Clofazimine is recommended as a treatment for tuberculosis in infants. Extemporaneously prepared clofazimine oral suspensions are more appropriate dosage forms infants than tablets, however no data exist to inform storage conditions. Aim To examine the physical and chemical stability of extemporaneously determine Method An suspension 10 mg/mL was by grinding six 100 mg tablets produce fine powder. Ora‐Blend SF (sugar free) added make final volume 60 mL. Six mL aliquots...
The encapsulation of liquid crystalline phases, formed from biocompatible amphiphiles, into nanoparticles has emerged as a promising delivery strategy for hydrophilic and hydrophobic therapeutics. Strategies to characterize these systems function formulation parameters aqueous environment post-manufacture are well-documented. A critical gap remains regarding the assembly kinetics in situ dynamics using industrially relevant manufacturing techniques. Systematically investigating...
This Technical Note presents the direct surface modification of a glass/PTFE hybrid microfluidic chip, via radio frequency glow discharge plasma polymerisation tetraethlylene glycol dimethylether (tetraglyme), to produce hydrophilic, non-fouling, PEO-like surfaces. We use several techniques including X-ray photoelectron spectroscopy (XPS), enzyme-linked immunosorbent assays (ELISA) and immunofluorescent imaging investigate channel coatings. Our results indicate successful deposition coating...
Abstract This paper presents a study of EOF properties plasma‐polymerized microchannel surfaces and the effects protein (fibrinogen lysozyme) adsorption on behavior surface‐modified microchannels. Three plasma polymer surfaces, i.e. tetraglyme, acrylic acid allylamine, are tested. Results indicate suppression in all plasma‐coated channels compared with uncoated glass surfaces. The behaviors modified microchannels after exposure to solutions also investigated show that even low levels can...
A series of novel branched-chain glycosides were synthesised from xylose, an aldopentose and Guerbet alcohols whose total number carbon atoms ranges C<sub>8</sub>–C<sub>24</sub>. Interestingly dry <italic>β</italic>-Xyl-C<sub>8</sub>C<sub>4</sub> gave a rectangular columnar ribbon phase at 25 °C.
Clofazimine, a drug previously used to treat leprosy, has recently been identified as potential new for the treatment cryptosporidiosis: diarrheal disease that contributes 500 000 infant deaths year in developing countries. Rapid dissolution and local availability of small intestine is considered key infection. However, commercially available clofazimine formulation (Lamprene) not well-suited pediatric use, therefore reformulation desirable. Development nanoparticles through process flash...
Techniques enabling in situ monitoring of drug solubilization and changes the solid-state during digestion milk milk-based formulations are valuable for predicting effectiveness such improving oral bioavailability poorly water-soluble drugs. We have recently reported use low-frequency Raman scattering spectroscopy (region analysis <200 cm–1) as an analytical approach to probe drugs using ferroquine (SSR97193) model compound. This study investigates wider utilization this technique behavior...
Drug overdose connected to marketed pharmaceutical products, particularly opioids, occurs at an alarming rate. Novel strategies through innovative formulation approaches that reduce the likelihood of while allowing safe therapeutic outcomes are urgently required. The current study provides a proof-of-concept for new approach by co-formulating drug with lipase inhibitor within solid lipid in order prevent or harmful effects taking multiple doses oral dose form. Lipase controlled-release...
Adsorption of biomolecules onto microchannel surfaces remains a critical issue in microfluidic devices. This paper investigates the adsorption fibrinogen on glass microcapillaries using an immunoassay method (ELISA) and X-ray photoelectron spectroscopy (XPS). Various conditions such as protein concentrations incubation times, buffer pH, ionic strengths effects flow are presented. ELISA is successfully demonstrated facile robust technique to examine these phenomena. The highest level occurs...
iTRAQ reagents allow the simultaneous multiplex identification and quantification of a large number proteins. Success depends on effective peptide fragmentation in order to generate both sequence ions (higher mass region, 150-2200 m/z) reporter (low 113-121 for protein relative quantification, respectively. After collision-induced dissociation, key requirements achieve good balance between high low m/z are ion transmission detection across MS/MS range, since higher range competes with that...