A5024060928- Immune responses and vaccinations
- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Inflammatory Bowel Disease
- Epigenetics and DNA Methylation
- Advanced Glycation End Products research
- Long-Term Effects of COVID-19
- T-cell and B-cell Immunology
- Microscopic Colitis
- Neuroinflammation and Neurodegeneration Mechanisms
- Xenotransplantation and immune response
- Celiac Disease Research and Management
- Circadian rhythm and melatonin
- Liver Disease and Transplantation
- Immune cells in cancer
- Eosinophilic Esophagitis
- Cancer Genomics and Diagnostics
- Vaccine Coverage and Hesitancy
- Cancer-related molecular mechanisms research
- Blood groups and transfusion
- Inflammasome and immune disorders
- Endometriosis Research and Treatment
Helmholtz Centre for Infection Research
2023-2025
Center for Experimental and Clinical Infection Research
2023-2025
Medizinische Hochschule Hannover
2023-2025
Centre for Individualised Infection Medicine
2023-2025
Qatif Central Hospital
2021-2022
Bacillus Calmette-Guérin (BCG) vaccination is a prototype model for the study of trained immunity (TI) in humans, and results more effective response innate immune cells upon stimulation with heterologous stimuli. Here, we investigate heterogeneity TI induction by single-cell RNA sequencing collected from 156 samples. We observe that both monocytes CD8+ T show transcriptional responses to lipopolysaccharide, an active crosstalk between these two cell types. Furthermore, interferon-γ pathway...
Aging affects human immune system functionality, increasing susceptibility to immune-mediated diseases. While gene expression programs accurately reflect function, their relationship with biological aging and health status remains unclear. Here we developed robust, cell-type-specific clocks (sc-ImmuAging) for the myeloid lymphoid cell populations in circulation within peripheral blood mononuclear cells, using single-cell RNA-sequencing data from 1,081 healthy individuals aged 18 97 years....
The Bacille Calmette-Guerin (BCG) vaccine is a well-established inducer of innate immune memory (also termed trained immunity), causing increased cytokine production upon heterologous secondary stimulation. Innate responses are known to be influenced by season, but whether seasons impact induction immunity not known. To explore the influence season on induced BCG vaccine, we vaccinated healthy volunteers with either during winter or spring. Three months later, measured ex vivo against...
Abstract Background Comprising about 40% of mucosal lymphocytes, γδ T cells represent a significant lineage in the gut, and thus are key players intestinal immune homeostasis. In contrast to αβ TCRs, TCRs able recognize antigen independent from MHC-restricted presentation. While antigens recognized by largely undefined, activation is initiated phospho-antigens members butyrophilin (BTN) family. Data both murine human studies suggest protective role pathogenesis inflammatory bowel diseases...
Gout, prevalent inflammatory arthritis caused by urate crystal deposition, involves immune cell activation, yet the precise role of CD14 monocytes in initiating response is poorly understood. This study aimed to characterise molecular and cellular landscape gout using single-cell transcriptomic analysis. Single-cell RNA sequencing was performed on peripheral blood mononuclear cells from 8 patients 6 age- sex-matched healthy controls. The findings were validated publicly available datasets....
Abstract The incidence of Lyme borreliosis has risen, accompanied by persistent symptoms. innate immune system and related cytokines are crucial in the host response symptom development. We characterized cytokine production capacity before after antibiotic treatment 1,060 patients. observed a negative correlation between antibody IL-10 responses, as well increased IL-1Ra responses patients with disseminated disease. Genome-wide mapping allowed us to identify 34 quantitative trait loci...
Abstract Epigenetic reprogramming plays an important role in shaping immune memory traits within both innate (trained immunity) and adaptive cells following Bacillus Calmette-Guérin (BCG) vaccination. However, the precise impact of dynamic DNA methylation alterations on immunological responses after BCG vaccination remains inadequately elucidated. To address this knowledge gap, we conducted a comprehensive study by integrating longitudinal analysis systems biology approaches. We established...
Multiplexed single-cell experiment designs are superior in terms of reduced batch effects, increased cost-effectiveness, throughput and statistical power. However, current computational strategies using genetics to demultiplex (sc) libraries limited when applied single-nuclei (sn) sequencing data (e.g., snATAC-seq snMultiome). Here, we present CellDemux: a framework for genetic demultiplexing within across modalities, including single-cell, paired snMultiome measurements. CellDemux uses...
The Bacille Calmette-Guerin (BCG) vaccine is a well-established inducer of innate immune memory (also termed trained immunity), causing increased cytokine production upon heterologous secondary stimulation. Innate responses are known to be influenced by season, but whether seasons impact induction immunity not known. To explore the influence season on induced BCG vaccine, we vaccinated healthy volunteers with either during winter or spring. Three months later, measured ex vivo against...
Abstract Biomarkers to identify ICU COVID-19 patients at high risk for mortality are urgently needed therapeutic care and management. Here we found plasma levels of the glycolysis byproduct methylglyoxal (MG) were 4.4-fold higher in upon admission that later died (n = 33), 1.7-fold survived 32),compared uninfected controls 30). The increased MG correlated inversely with MG-degrading enzyme glyoxalase-1 ( r 2 − 0.50), its co-factor glutathione 0.63), positively monocytes 0.29). inflammation...
SUMMARY Vaccination-induced protection against influenza is greatly diminished and increasingly heterogeneous with age. We investigated longitudinally (up to five timepoints) a cohort of 234 elderly vaccinees across two independent seasons including up six modalities (multi-omics immunological parameters). System-level analyses revealed responders exhibited time-dependent changes attributed productive vaccine response all omics layers whereas non-responders did not follow such dynamics,...
Abstract Background Inflammatory bowel diseases (IBD), including Ulcerative colitis (UC) and Crohn's disease (CD), are chronic intestinal disorders characterised by varied symptoms a negative impact on the patients` quality of life. Increasing evidence demonstrates sex differences regarding prevalence, pathophysiology, clinical presentation treatment outcome IBD. While for some factors is rather good, others data conflicting – thus deeper understanding related in IBD courses still needed....
Background&Aims: The role of antibody-mediated rejection (ABMR) after liver transplantation (LT) remains controversial. Chronic ABMR (cABMR) is often subclinical and potentially missed without surveillance biopsies (svLbx). Transcriptome analysis previously characterized molecular changes in T cell-mediated (TCMR) solid organ transplantation. We aimed to identify cABMR signatures LT. Methods: Indication svLbx from two prospective institutional biorepositories were screened. performed...
Vaccination-induced protection against influenza is greatly diminished and increasingly heterogeneous with age. We investigated longitudinally (up to five time points) a cohort of 234 vaccinated >65-year-old vaccinees adjuvanted vaccine FluAd across two independent seasons. System-level analyses multiomics datasets measuring six modalities serological data revealed that poor responders lacked time-dependent changes in response vaccination as observed responders, suggestive systemic...
Abstract Biomarkers to identify ICU COVID-19 patients at high risk for mortality are urgently needed therapeutic care and management. In this study we found that plasma levels of the glycolysis byproduct methylglyoxal (MG) were 4.4-fold 1.7-fold higher (P<0.0001) upon admission in later died (n=34) compared uninfected controls (n=30) those survived (n=31), respectively. The increase MG was inversely correlated with glutathione ( r 2 =-0.63) MG-glutathione degrading glyoxalase-1 =-0.50),...