A5009907429- Herpesvirus Infections and Treatments
- Cholesterol and Lipid Metabolism
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Cervical Cancer and HPV Research
- Systemic Lupus Erythematosus Research
- Infection Control and Ventilation
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- HIV Research and Treatment
- Proteoglycans and glycosaminoglycans research
- COVID-19 diagnosis using AI
- Advanced Drug Delivery Systems
- COVID-19 Impact on Reproduction
- Infant Nutrition and Health
- Peroxisome Proliferator-Activated Receptors
- Biosensors and Analytical Detection
- Peripheral Neuropathies and Disorders
- Congenital Anomalies and Fetal Surgery
University of Turin
2016-2025
Ospedale San Luigi Gonzaga
2009-2022
Ospedale Amedeo di Savoia
2022
Viral infections represent a public health problem and one of the leading causes global mortality. Nanomedicine strategies can be considered powerful tool to enhance effectiveness antiviral drugs, often associated with solubility bioavailability issues. Consequently, high doses frequent administrations are required, resulting in adverse side effects. To overcome these limitations, various nanomedicine platforms have been designed.This review focuses on state art organic-based nanoparticles...
Abstract Up to 80% of the cost vaccination programmes is due cold chain problem (that is, keeping vaccines cold). Inexpensive, biocompatible additives slow down degradation virus particles would address problem. Here we propose and characterize that, already at very low concentrations, improve storage time adenovirus type 5. Anionic gold nanoparticles (10 −8 –10 −6 M) or polyethylene glycol (PEG, molecular weight ∼8,000 Da, 10 −7 −4 increase half-life a green fluorescent protein expressing...
Abstract Recent studies reported a broad but selective antiviral activity of 25-hydroxycholesterol (25HC) against enveloped viruses, being apparently inactive non-enveloped viruses. Here we show that 25HC is endowed with marked three pathogenic i.e. human papillomavirus-16 (HPV-16), rotavirus (HRoV) and rhinovirus (HRhV), thus significantly expanding its spectrum, so far recognized to be limited viruses envelope. Moreover, here disclose the remarkable another oxysterol physiological origin,...
There is an urgent need to identify antivirals against the coronavirus SARS-CoV-2 in current COVID-19 pandemic and contain future similar emergencies early on. Specific side-chain cholesterol oxidation products of oxysterols family have been shown inhibit a large variety both enveloped non-enveloped human viral pathogens. Here we report on vitro inhibitory activity redox active oxysterol 27-hydroxycholesterol one common cold agents HCoV-OC43 without significant cytotoxicity. Interestingly,...
Acyclovir is not a good candidate for passive permeation since its polarity and solubility limit partitioning into the stratum corneum. This work aims to develop new topical formulation acyclovir delivery. New chitosan nanospheres (NS) were prepared by modified nano-emulsion template method. Chitosan NS characterized Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), an in vitro release study. The skin experiment was carried out using Franz cells equipped with porcine...
A novel innate immune strategy, involving specific cholesterol oxidation products as effectors, has begun to reveal connections between metabolism and response against viral infections. Indeed, 25-hydroxycholesterol (25HC) 27-hydroxycholesterol (27HC), physiologically produced by enzymatic of cholesterol, act inhibitors a wide spectrum enveloped non-enveloped human viruses. However, the mechanisms underlying their protective effects viruses are almost completely unexplored. To get insight...
The airborne transmission of SARS-CoV-2 remains surprisingly controversial; indeed, health and regulatory authorities still require direct proof this mode transmission. To close gap, we measured the viral load an infected subject in a hospital room (through oral nasopharyngeal swab), as well concentration resulting from person breathing speaking. Moreover, simulated same scenarios to estimate RNA copies air through novel theoretical approach conducted comparative analysis between...
Oxysterols are known pleiotropic molecules whose antiviral action has been recently discovered. Here reported is the activity of a panel oxysterols against HSV-1 with identification new mechanism action. A marked not only 25HC but also 27HC was observed either if were added before or after infection, suggesting an unrelated to viral entry inhibition as proposed by previous literature. Therefore, relation between pro-inflammatory and activation NF-kB IL-6 induced in host cell investigated....
ABSTRACT Peptide dendrimers consist of a peptidyl branching core and/or covalently attached surface functional units. They show variety biological properties, including antiviral activity. In this study, minilibrary linear, dimeric, and dendrimeric peptides containing clusters basic amino acids was evaluated for in vitro activity against human papillomaviruses (HPVs). The peptide dendrimer SB105-A10 found to be potent inhibitor genital HPV types (i.e., 16, 18, 6) pseudovirus-based...
ABSTRACT Respiratory syncytial virus (RSV) interacts with cell surface heparan sulfate proteoglycans (HSPGs) to initiate infection. The interaction of RSV HSPGs thus presents an attractive target for the development novel inhibitors In present study, a minilibrary linear, dimeric, and dendrimeric peptides containing clusters basic amino acids was screened aim identifying able bind block attachment infectivity. Of compounds identified, dendrimer SB105-A10 most potent inhibitor infectivity,...
Abstract Zika virus, an arthropod-borne flavivirus, is emerging healthcare threat worldwide. virus responsible for severe neurological effects, such as paralytic Guillain-Barrè syndrome, in adults, and also congenital malformations, especially microcephaly. No specific antiviral drugs vaccines are currently available, treatments palliative, but medicinal plants show great potential natural sources of anti-Zika phytochemicals. This study deals with the investigation composition, cytotoxicity,...
Human respiratory viruses have an enormous impact on national health systems, societies, and economy due to the rapid airborne transmission epidemic spread of such pathogens, while effective specific antiviral drugs counteract infections are still lacking. Here, we identified two Keggin-type polyoxometalates (POMs), [TiW11CoO40]8- (TiW11Co) [Ti2PW10O40]7- (Ti2PW10), endowed with broad-spectrum activity against enveloped non-enveloped human viruses, i.e., coronavirus (HCoV-OC43), rhinovirus...
ABSTRACT Respiratory syncytial virus (RSV) exploits cell surface heparan sulfate proteoglycans (HSPGs) as attachment receptors. The interaction between RSV and HSPGs thus presents an attractive target for the development of novel inhibitors infection. In this study, selective chemical modification Escherichia coli K5 capsular polysaccharide was used to generate a collection sulfated derivatives with backbone structure that mimics heparin/heparan biosynthetic precursor. screening series...
Thymus capitatus represents 1 of the 5 Tunisian species genus , which has long‐standing use for flavouring and preserving several food products. Its constituents have been reported to endow antimicrobial properties, but little is known about their antiviral activities. The aim this study was examine activity pure compounds from most bioactive inhibitory T. extract in vitro against herpes simplex virus Type 2 (HSV‐2) infection identify mechanism action. Either extracts or essential oil exert...
Infections caused by HSV-2 are a public health concern worldwide, and there is still great demand for the discovery of novel anti-herpes virus agents effective against strains resistant to current antiviral agents. In this context, medicinal plants represent an alternative source active compounds developing efficient therapies. The aim study was evaluate activity Arisaema tortuosum, plant used in traditional medicine India. A chloroform soluble fraction leaves exhibited anti-HSV-2 with...