A5016654292- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Nutrition and Health in Aging
- Genetic Neurodegenerative Diseases
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Sports Performance and Training
- Pancreatic function and diabetes
- Histone Deacetylase Inhibitors Research
- Sirtuins and Resveratrol in Medicine
- Exercise and Physiological Responses
- Advanced Neuroimaging Techniques and Applications
- Cannabis and Cannabinoid Research
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Body Composition Measurement Techniques
- Sports injuries and prevention
- Nutritional Studies and Diet
- Blood properties and coagulation
- Action Observation and Synchronization
- Methemoglobinemia and Tumor Lysis Syndrome
- Coenzyme Q10 studies and effects
- Bone health and osteoporosis research
- Mechanical and Thermal Properties Analysis
- Erythrocyte Function and Pathophysiology
Cornell University
2022-2024
Weill Cornell Medicine
2022-2024
University of California, Davis
2019-2023
Charité - Universitätsmedizin Berlin
2017-2022
University of California Davis Medical Center
2021
University of California System
2021
Humboldt-Universität zu Berlin
2020
Freie Universität Berlin
2020
Max Delbrück Center
2017-2018
Maastricht University Medical Centre
2016
<b><i>Background: </i></b>Anemia is a major complication of end stage renal disease. The anemia mainly the result impaired formation erythrocytes due to lack erythropoietin and iron deficiency. Compelling evidence, however, points contribution accelerated erythrocyte death, which decreases life span circulating erythrocytes. Erythrocytes may enter suicidal death or eryptosis, characterized by cell shrinkage membrane scrambling with phosphatidylserine-exposure at...
Introduction We have previously shown that protein ingestion before sleep increases overnight muscle synthesis rates. Whether prior exercise further augments the synthetic response to presleep remains be established. Objective This study aimed assess whether resistance-type performed in evening ingestion. Methods Twenty-four healthy young men were randomly assigned ingest 30 g intrinsically L-[1-13C]-phenylalanine and L-[1-13C]-leucine-labeled casein going with (PRO + EX, n = 12) or without...
Muscle loss is a severe complication of many medical conditions such as cancer, cardiac failure, muscular dystrophies, and nerve damage. The contribution myofibrillar protein synthesis (MPS) to the muscle mass after damage not clear. Using deuterium oxide (D2O) labeling, we demonstrate that MPS significantly increased in rat m.tibialis anterior (TA) compared control (3.23 ± 0.72 [damaged] 2.09 0.26%∗day-1 [control]) 4 weeks constriction injury. This case despite substantial TA (350 96 mg 946...
Implement STEAM-DTI to model time-dependent diffusion eigenvalues using the random permeable barrier (RPBM) study age-related differences in medial gastrocnemius (MG) muscle. Validate model-extracted fiber diameter for histological assessment.
To investigate systemic regulators of the cancer-associated cachexia syndrome (CACS) in a pre-clinical model for lung cancer with goal to identify therapeutic targets tissue wasting.
The molecular responses to acute resistance exercise are well characterized. However, how cellular signals change over time modulate chronic adaptations more prolonged training is less understood. We investigated anabolic signaling and muscle protein synthesis rates at several points after eccentric loading. Adult rat tibialis anterior was stimulated for six sets of ten repetitions, the collected 0 h, 6 18 h 48 h. In last group animals, first bout a second conducted, later (54 total)....
Abstract Perturbations in skeletal muscle metabolism have been reported for a variety of neuromuscular diseases. However, the role after constriction injury to nerve and associated atrophy is unclear. We analyzed rat tibialis anterior (TA) four weeks unilateral sciatic (DMG) contralateral control leg (CTRL) (n = 7) investigate changes metabolome, immunohistochemistry protein levels. Untargeted metabolomics identified 79 polar metabolites, 27 which were significantly altered DMG compared...
Fracture induces systemic bone loss in mice and humans, a first (index) fracture increases the risk of future at any skeletal site more men than women. The etiology this sex difference is unknown, but may greater response men. Also differences muscle after have not been examined. We investigated transverse femur 3-month-old male female C57BL/6 J mice. Whole-body regional mineral content density (BMC BMD), trabecular cortical microstructure, contractile force, mass, fiber size were quantified...
Abstract Background Desminopathy is a clinically heterogeneous muscle disease caused by over 60 different mutations in desmin. The most common mutation with clinical phenotype humans an exchange of arginine to proline at position 350 desmin leading p.R350P. We created the first CRISPR‐Cas9 engineered rat model for mirroring R350P humans. Methods Using technology, Des c.1045‐1046 (AGG > CCG) was introduced into exon 6 genome causing p.R349P. genotype each animal confirmed via quantitative...
Abstract The tumor suppressor p53 is thought to play a key role in the maintenance of cell size and homeostasis, but relatively little known about its skeletal muscle. Based on ability suppress growth, we hypothesized that inhibiting function wild-type through overexpression dominant-negative mutant (DDp53) could result muscle fiber hypertrophy. To test this hypothesis, electroporated adult rat tibialis anterior muscles with DDp53 collected tissue three weeks later. We confirmed successful...
Abstract The cancer associated cachexia syndrome (CACS) is a systemic metabolic disorder resulting in loss of body weight due to skeletal muscle and adipose tissues atrophy. CACS particularly prominent lung patients, where it contributes poor quality life excess mortality. Using the Kras/Lkb1 (KL) mouse model, we found that with white tissue (WAT) dysfunction directly affects homeostasis. WAT transcriptomes showed evidence reduced adipogenesis, and, agreement, low levels circulating...
The ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway are primary means of degradation in mammalian tissues. We sought to determine individual contribution UPS autophagy tissue catabolism during fasting. Mice were overnight fasted for 15 h before regaining food access ("Fed" group,
Cannabidiol (CBD) has proven clinical benefits in the treatment of seizures, inflammation, and pain. The recent legalization CBD many countries caused increased interest drug as an over-the-counter for athletes looking to improve recovery. However, no data on effects adaptive response exercise muscle are available. To address this gap, we eccentrically loaded tibialis anterior 14 rats, injected them with a vehicle (n = 7) or 100 mg/kg 7), measured markers injury, anabolic signaling,...
Abstract Desminopathy is the most common intermediate filament disease in humans. The frequent mutation causing desminopathy patients a R350P DES missense mutation. We have developed rat model with an analogous R349P Des . To investigate role of mechanical loading, we stimulated sciatic nerve wild‐type littermates (WT) (n = 6) and animals carrying (MUT) lengthening contraction dorsi flexor muscles. MUT showed signs ongoing regeneration at baseline as indicated by higher number central nuclei...
Background: Cannabidiol (CBD) is becoming increasingly popular for the treatment of clinical conditions including as an aid muscle recovery. Previous work demonstrated that CBD exhibited mild effects on skeletal muscle, with a tendency to increase anabolic signaling and decrease inflammatory signaling. Methods: To gain mechanistic insight extend these findings, we conducted set experiments using C2C12 myotubes. Results: Increasing dose (1–5 μM) provided insulin-like growth factor 1 (IGF-1)...
Cachexia is a debilitating comorbidity affecting many lung cancer patients. We have previously found that cachectic mice with reduced serum ketone body levels due to low PPARα activity in the liver. Restoring hepatic fenofibrate increased circulating ketones and delayed muscle white adipose tissue wasting. hypothesized loss of plays pathophysiologic role cachexia performed two dietary intervention studies test this hypothesis. In first study, male female were randomized consume either very...
THIS ARTICLE REVIEWS THE AVAILABLE LITERATURE ON WHICH PROTEINS, AMINO ACIDS, OR COMBINATION OF BOTH SEEM TO BE OPTIMAL ENHANCE HYPERTROPHY AFTER RESISTANCE EXERCISE IN YOUNG ADULTS. DEPENDING CONTENT ESSENTIAL ACIDS AND PARTICULARLY LEUCINE, EITHER AN IMMEDIATE INGESTION ∼20 G MILK PROTEIN FOLLOWED BY A SIMILAR AMOUNT ∼1 HOUR LATER, SINGLE BOLUS ∼40 SEEMS SUITABLE. GREATER AMOUNTS MIGHT NECESSARY IF LOWER QUALITY IS CHOSEN (I.E., PLANT-BASED PROTEINS) MATCH REQUIRED ACID QUANTITIES...
ABSTRACT Desminopathy the most common intermediate filament disease in humans. Desmin is an essential part of filamentous network that aligns myofibrils, anchors nuclei and mitochondria, connects z-discs sarcolemma. We created a rat model with mutation R349P DES , analog to frequent R350P missense To examine effects chronic, physiological exercise stimulus on desminopathic muscle, we subjected rats their wildtype (WT) heterozygous littermates treadmill running regime. saw significantly lower...