A5085181222- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- GDF15 and Related Biomarkers
- Heart Failure Treatment and Management
- Prostate Cancer Treatment and Research
- Galectins and Cancer Biology
- Cancer Genomics and Diagnostics
- Ethics in Clinical Research
- Nutrition and Health in Aging
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Asthma and respiratory diseases
- Cardiac Fibrosis and Remodeling
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Sex and Gender in Healthcare
- Reproductive Biology and Fertility
Tataa Biocenter (Sweden)
2014-2023
Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for detection of nucleic acids. Even though it has been around more than 30 years preferred in research applications, yet to win broad acceptance routine practice. This requires a means unambiguously assess performance qPCR analyses. Here present methods determine limit (LoD) quantification (LoQ) applicable qPCR. These are based on standard statistical recommended by...
Background Identifying the mechanistic pathways potentially associated with incident heart failure (HF) may provide a basis for novel preventive strategies. Methods and Results To identify proteomic biomarkers potential underlying that be HF defined as first hospitalization HF, nested-matched case-control design was used cases (incident HF) controls (without selected from 3 cohorts (>20 000 individuals). Controls were matched on cohort, follow-up time, age, sex. Two independent sample sets...
Background: We sought to identify protein biomarkers of new-onset heart failure (HF) in 3 independent cohorts (HOMAGE cohort [Heart Omics and Ageing], ARIC study [Atherosclerosis Risk Communities], FHS [Framingham Heart Study]) assess if what extent they improve HF risk prediction compared clinical factors alone. Methods: A nested case-control design was used with cases (incident HF) controls (without matched on age sex within each cohort. Plasma concentrations 276 proteins were measured at...
// Andreas Josefsson 1 , Karin Larsson Marianne Månsson Jens Björkman 3 Eva Rohlova 3, 4, 5, 6 Daniel Åhs Helena Brisby 2 Jan-Erik Damber and Welén Sahlgrenska Cancer Center, Department of Urology, Institute Clinical Sciences, Academy, University Gothenburg, Sweden Orthopaedics, TATAA Biocenter AB, 4 Anthropology Human Genetics, Faculty Science, Charles University, Prague, Czech Republic 5 Laboratory Gene Expression, Biotechnology CAS, BIOCEV, Vestec, Centre for Experimental Medicine,...
There is an increasing need for proper quality control tools in the pre-analytical phase of molecular diagnostic workflow. The aim present study was to identify biomarkers monitoring mRNA variations two different types blood collection tubes, K2EDTA (EDTA) tubes and PAXgene Blood RNA Tubes (PAXgene tubes). These are extensively used both setting as well research biobank samples. specimens collected were stored varying times at temperatures, microarray analysis performed on resultant...
Integrity of the mRNA in clinical samples has major impact on quality measured expression levels. This is independent measurement technique being next generation sequencing (NGS), Quantitative real-time PCR (qPCR) or microarray profiling. If highly degraded damaged, data will be very unreliable and whole study likely a waste time money. It therefore common strategy to test RNA before conducting large costly studies. Most methods today assess are ignorant nature and, therefore, reflect...
Heart failure (HF) is common in both men and women, yet disease pathophysiology, presentation, progression differ between sexes. Studies addressing whether biomarkers predict new onset HF sex-specifically are scarce. This study therefore aims to test the sex-specificity of 252 protein for new-onset HF.A matched case-control design patients selected from cohorts within HOMAGE consortium was used. Cases (new-onset HF, n = 562) controls (n 780) were cohort (PREDICTOR, HEALTH-ABC, & PROSPER),...
Abstract Successful molecular analyses of human solid tissues require intact biological material with well-preserved nucleic acids, proteins, and other cell structures. Pre-analytical handling, comprising the collection at operating theatre, is among first critical steps that influence sample quality. The aim this study was to compare experimental outcomes obtained from samples collected stored by conventional means snap freezing PAXgene Tissue System (Qiagen). These approaches were...
Abstract The TCGA, ICGC and other research consortiums has shown that an integral, multi-omic analysis of tumors is necessary in order to understand a more complete picture the signaling pathways involved development, evolution, prognosis treatment resistance tumors. However, such studies, because different data modalities (“omics”) are analyzed separately, information on how co-existence same cell lacking. We report new approach measure multiple simultaneously from up 10,000 individual...