A5102862057- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Aquaculture disease management and microbiota
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Polydiacetylene-based materials and applications
- Plant Virus Research Studies
- Ocular Surface and Contact Lens
- Viral gastroenteritis research and epidemiology
- Aquaculture Nutrition and Growth
- Advancements in Transdermal Drug Delivery
- HIV Research and Treatment
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
National Taiwan University
2017-2025
Beijing Forestry University
2025
National Chung Hsing University
2011-2024
National Dong Hwa University
2011-2017
National Defense Medical Center
2017
Mennonite Christian Hospital
2017
Tri-Service General Hospital
2017
Tungs' Taichung MetroHarbor Hospital
2017
Chang Gung University
2017
Centers for Disease Control and Prevention
2011
Portable biosensors mainly focus on detecting biomarkers in biofluids but neglect the abundant skin stratum corneum, which are associated with functionality and integrity of barrier. Here, we propose a sensing patch designed for direct sampling situ quantification epidermal serine, an important biomarker healthcare. The consists porous hydrogel serine diffusion ion conduction, molecular imprinted polymer-based electrochemical sensor. By integrating customized handheld tester, system enables...
Bovine lactoferrin (bLF) presents in milk and has been shown to inhibit several viral infections. Effective drugs are unavailable for the treatment of dengue virus (DENV) infection. In this study, we evaluated antiviral effect bLF against DENV infection vivo vitro. LF significantly inhibited four serotypes Vero cells. time-of-drug addition test, DENV-2 was remarkably when added during or prior occurrence attachment. We also revealed that bovine blocks binding between cellular membrane by...
Formalin-inactivated Japanese encephalitis virus (JEV) vaccines are widely available, but the effects of formalin inactivation on antigenic structure JEV and profile antibodies elicited after vaccination not well understood. We used a panel monoclonal (MAbs) to map live virus, untreated control (UCV), formalin-inactivated commercial vaccine (FICV), (FIV). The binding activity T16 MAb against Nakayama-derived FICV several strains FIV was significantly lower compared UCV. MAb, weakly...
Abstract Genotype I of Japanese encephalitis virus first appeared in Taiwan 2008. Phylogenetic analysis 37 viruses from pig farms 2009–2010 classified these into 2 unique subclusters genotype and suggested multiple introductions swift replacement III by Taiwan.
Background Genotype I (GI) Japanese encephalitis virus (JEV) that replaced GIII has become the dominant circulating in Asia. Currently, all registered live and inactivated JEV vaccines are derived from genotype III viruses. In Taiwan, compulsory vaccination policy recommends children receives four doses of formalin-inactivated Nakayama (GIII) vaccine. Methodology/Principal Findings To evaluate influence replacement on post-vaccination viral neutralizing ability by GI viruses, small panel...
Group and serocomplex cross-reactive epitopes have been identified in the envelope (E) protein of several flaviviruses proven critical vaccine diagnostic antigen development. Here, we performed site-directed mutagenesis across E gene a recombinant expression plasmid that encodes Japanese encephalitis virus (JEV) premembrane (prM) proteins produces JEV virus-like particles (VLPs). Mutations were introduced at I135 E138 domain I; W101, G104, G106 L107 II; T305, E306, K312, A315, S329, S331,...
Expansion of genotype I (GI) Japanese encephalitis viruses (JEV) has resulted in the replacement dominant III (GIII) viruses, raising serious public health concerns for using GIII virus-derived vaccines to effectively control JEV epidemics. Therefore, this study used swine as model estimate effectiveness live-attenuated vaccine against GI virus infection by comparing incidence stillbirth/abortion gilts from vaccinated and non-vaccinated pig farms during GI-circulation period. In total, 389...
Abstract Tick-borne severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease caused by the SFTS virus (SFTSV). Serological assays based on nucleocapsid protein and partial glycoprotein of this have been used for detecting SFTSV infections in humans animals. However, whether complete (Gn/Gc) can induce assembly virus-like particles (VLPs) which be serological surveillance vaccine production remains unclear. In study, we successfully expressed secreted Gn/Gc antigens...
Abstract Flavivirus virus-like particles (VLPs) exhibit a striking structural resemblance to viral particles, making them highly adaptable for various applications, including vaccines and diagnostics. Consequently, increasing VLPs production is important can be achieved by optimizing expression plasmids cell culture conditions. While attempting express genotype III (GIII) Japanese encephalitis virus (JEV) containing the G104H mutation in envelope (E) protein, we failed generate COS-1 cells....
Genotype I (GI) virus has replaced genotype III (GIII) as the dominant Japanese encephalitis (JEV) in epidemic area of Asia. The mechanism underlying replacement remains unclear. Therefore, we focused our current study on investigating roles mosquito vector and amplifying host(s) JEV by comparing replication ability GI GIII viruses. viruses had similar infection rates replicated to viral titers after blood meal feedings Culex tritaeniorhynchus. However, yielded a higher titer host-derived...
Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection important for the current genotype III (GIII) virus-derived vaccines swine, especially now that emerging I (GI) JE virus (JEV) has replaced GIII as dominant strain. Herein, we aimed to develop system generate GI JEV virus-like particles (VLPs) evaluate of vaccine candidate mice specific pathogen-free swine. A CHO-heparan sulfate-deficient...
Virologic surveillance of Japanese encephalitis virus (JEV) relies on collecting pig blood specimens and adult mosquitoes in the past. Viral RNAs extracted from suffer low detecting positivity by reverse transcription PCR (RT-PCR). The oronasal transmission has been demonstrated experimentally infected pigs. This observation suggested could be useful source surveillance. However, role this unusual route remains unproven operational farm. In study, we explore feasibility using secretions...
The virulence of genotype I (GI) Japanese encephalitis virus (JEV) is under debate. We investigated differences in the GI and GIII JEV by calculating asymptomatic ratios based on serologic studies during GI- GIII-JEV endemic periods. results suggested equal among humans.
Salmonella genomic island 1 was identified for the first time in enterica serovar Virchow isolated from humans Taiwan. The complex class integron conferring multidrug resistance shown to be inserted within open reading frame (ORF) S023 and contains a partial transpositional module. 5-bp target duplication flanking suggests that its insertion ORF by transposition.
Japanese encephalitis (JE) is a disease that threatens both human and animal populations in Asian countries, the causative agent of JE, virus (JEV), has recently changed from genotype III (GIII) to I (GI). However, test for rapid differentiation GI GIII JEV still unavailable, especially one can be used mosquito-based surveillance. We have designed GI- GIII-specific primer sets detection by multiplex reverse transcriptase-polymerase chain reaction (multiplex RT-PCR). The GI-specific were able...
ABSTRACT Dengue vaccine candidates have been shown to improve safety and efficacy by altering the residues or accessibility of fusion loop on virus envelope protein domain II (DII FL ) in an ex vivo animal study. The current study aimed comprehensively investigate impact DII mutations antigenicity, immunogenicity, protective Japanese encephalitis (JEV) virus-like particles (VLPs) mice. We found G106K/L107D (KD) W101G/G106K/L107D (GKD) altered binding activity JEV VLP cross-reactive...
Taiwan's experience with severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 guided its development of strategies to defend against SARS-CoV-2 2020, which enabled the successful control Coronavirus disease 2019 (COVID-19) cases from 2020 through March 2021. However, late-April 2021, imported Alpha variant began cause COVID-19 outbreaks at an exceptional rate Taiwan. In this study, we aimed determine what epidemiological conditions strains become dominant and decline later during...