A5052082771- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory viral infections research
- Whipple's Disease and Interleukins
- Transgenic Plants and Applications
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- Plant Virus Research Studies
- Neuroendocrine Tumor Research Advances
- Mosquito-borne diseases and control
- Fungal Infections and Studies
- CRISPR and Genetic Engineering
- Amoebic Infections and Treatments
- Reproductive Physiology in Livestock
- Probiotics and Fermented Foods
- Orthopedic Infections and Treatments
- Microbial Metabolites in Food Biotechnology
- Gestational Trophoblastic Disease Studies
- Nail Diseases and Treatments
National Chung Hsing University
2017-2023
National Health Research Institutes
2012-2022
National Taipei University
2020
National Taiwan University
2005-2014
Taipei Institute of Pathology
2009-2011
Background Recently, enterovirus 71 (EV71) has caused life-threatening outbreaks involving neurological and cardiopulmonary complications in Asian children with unknown mechanism. EV71 one single serotype but can be phylogenetically classified into 3 main genogroups (A, B C) 11 genotypes B1∼B5 C1∼C5). In Taiwan, nationwide epidemics different predominant occurred 1998 (C2), 2000–2001 (B4), 2004–2005 (C4), 2008 (B5). this study, sera were collected to measure cross-reactive neutralizing...
Bovine enteric bacterial pathogens are a major cause of health decline in agricultural cattle populations. The identification host-derived microbiota with probiotic characteristics is key for the development treatments utilizing pathogen displacement and recolonization by commensal flora. In this study, intestinal fecal samples from four Holstein dairy cows were analyzed using 16S ribosomal RNA gene next-generation sequencing, leading to three Lactobacillus isolates (Lactobacillus gasseri,...
Abstract The Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), is important tool for the prevention and control CSFV infection widely routinely used in most CSF endemic areas, including Taiwan. aim this study was to investigate whether PCV2 affects efficacy LPC vaccine. Eighteen 6-week-old, cesarean-derived colostrum-deprived (CDCD), crossbred pigs were randomly assigned four groups. A total 10 5.3 TCID 50 experimentally inoculated into...
New variants of porcine epidemic diarrhoea virus (PEDV) causing a highly contagious intestinal disease, (PED), have resulted in high mortality suckling pigs across several countries since 2013. After 2015, the prevalence genogroup 2b (G2b) PEDVs decreased cyclical pattern with endemic seasonal outbreaks occasionally seen. To better understand genetic diversity recently circulating Taiwan, full-length spike (S) genes 31 PEDV strains from 28 pig farms collected during 2016-2018 were sequenced....
Enterovirus A71 (EV-A71) is one of the major causes hand, foot, and mouth disease (HFMD), primarily affecting children under five. It can lead to neurological cardiac complications, or even death, in some cases. Inactivated monovalent vaccines have been licensed China Taiwan; however, stability EV-A71 often compromised by factors such as extreme temperatures ultraviolet (UV) irradiation. Currently, no commercially available tools assess throughout vaccine development. In this study, we...
Avian-origin influenza A (H7N9) viruses emerged as human pathogens in China early 2013 and have killed >100 persons. Influenza vaccines are mainly manufactured using egg-based technology which could not meet the surging demand during pandemics. In this study, we evaluated cell-based H7N9 ferrets. An egg-derived reassortant vaccine virus was adapted MDCK cells. whole antigen a microcarrier-based culture system. Immunogenicity protection of candidates with three different formulations (300μg...
Amoebiasis is one of the most common protozoal diseases reptiles, but amoebic myositis has not been reported in any animal species. An 11-year-old, male water monitor lizard (Varanus salvator) was found dead with several subacute ulcerated skin wounds. Gross examination revealed multiple discrete to coalescing, white-yellow gray, caseous foci scattered skeletal muscles and liver. The mucosa small intestine thickened, red, contained many variably sized, dark red ulcers, depressed hemorrhagic...
Summary Porcine reproductive and respiratory syndrome virus ( PRRSV ) is a persistent threat of economically significant influence to the swine industry worldwide. Recombinant DNA technology coupled with tissue culture viable alternative for inexpensive production heterologous proteins in planta . Embryogenic cells banana cv. ‘Pei chiao’ AAA have been transformed ORF 5 gene envelope glycoprotein GP 5) using Agrobacterium ‐mediated transformation confirmed. protein levels transgenic leaves...
Background Enterovirus 71 (EV71) belongs to picornavirus family and could be classified phylogenetically into three major genogroups (A, B C) including 11 genotypes B1-B5 C1-C5). Since 1997, EV71 has caused large-scale of epidemics with neurological complications in Asian children. In Taiwan, nationwide different predominant have occurred cyclically since 1998. A epidemic again 2012. We conducted genetic antigenic characterizations the 2012 epidemic. Methods Chang Gung Memorial Hospital...
Virologic surveillance of Japanese encephalitis virus (JEV) relies on collecting pig blood specimens and adult mosquitoes in the past. Viral RNAs extracted from suffer low detecting positivity by reverse transcription PCR (RT-PCR). The oronasal transmission has been demonstrated experimentally infected pigs. This observation suggested could be useful source surveillance. However, role this unusual route remains unproven operational farm. In study, we explore feasibility using secretions...
Enterovirus 71 (EV71) causes life-threatening epidemics in Asia and can be phylogenetically classified into three major genogroups (A∼C) including 11 genotypes (A, B1∼B5, C1∼C5). Recently, EV71 occurred cyclically Taiwan with different genotypes. In recent years, human studies using post-infection sera obtained from children have detected antigenic variations among strains. Therefore, surveillance of enterovirus should include phylogenetic analysis. Due to limitation available primary...
Abstract Classical swine fever virus (CSFV) infection leading to CSF outbreaks is among the most devastating diseases in pig industry. Porcine circovirus type 2 (PCV2) infection, resulting porcine circovirus-associated disease (PCVAD), also a highly contagious affecting health worldwide. To prevent and control occurrence, multiple-vaccine immunization necessary contaminated areas or countries. In this study, novel CSFV-PCV2 bivalent vaccine was constructed demonstrated be capable of...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most common diseases in global swine industry. PRRSV characterized by rapid mutation rates extensive genetic divergences. It divided into two genotypes, which are composed several distinct sub-lineages. The purpose present study was to evaluate cross-protective efficacy Fostera PRRS MLV, an attenuated lineage 8 strain, against heterologous challenge a 3 isolate. Eighteen pigs were randomly mock, MLV unvaccinated (UnV) groups....