A5045509209- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Occupational and environmental lung diseases
- Reproductive Biology and Fertility
- Bacteriophages and microbial interactions
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Effects of Environmental Stressors on Livestock
- Sperm and Testicular Function
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Liver Disease and Transplantation
- Respiratory viral infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Airway Management and Intubation Techniques
- COVID-19 Clinical Research Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pharmacological Receptor Mechanisms and Effects
- SARS-CoV-2 detection and testing
- Pleural and Pulmonary Diseases
- Biomarkers in Disease Mechanisms
- Coronary Interventions and Diagnostics
- Liver physiology and pathology
Children's Healthcare of Atlanta
2020-2023
Emory University
2011-2023
McMaster University
2022
Burton Hospitals NHS Foundation Trust
2019
Queen's Hospital
2019
National Dairy Research Institute
2014-2018
Indian Veterinary Research Institute
2014-2017
Junagadh Agricultural University
2014
Georgetown University Medical Center
1994
Georgetown University
1994
Hepatitis B virus (HBV) affects an estimated 250 million chronic carriers worldwide. Though several vaccines exist, they are ineffective for those already infected. HBV persists due to the formation of covalently closed circular DNA (cccDNA)—the viral minichromosome—in nucleus hepatocytes. Current nucleoside analogs and interferon therapies rarely clear cccDNA, requiring lifelong treatment.
To characterize the physico-chemical properties of estrual cervical mucus among different parities and analyse their association with conception rate in Murrah buffaloes.Cervical was collected from mid-cervix using sterile blue sheath before artificial insemination (AI) buffaloes (n=94) examined for appearance (transparent/ translucent), consistency (thin/ moderate/ thick), Spinnbarkeit value, arborisation pattern (typical/ atypical/ nil), pH electrical conductivity.Artificial carried out...
Remdesivir, a monophosphate prodrug of nucleoside analog GS-441524, is widely used for the treatment moderate to severe COVID-19. It has been suggested use GS-441524 instead remdesivir in clinic and new inhalation formulations. Thus, we compared anti-SARS-CoV-2 activity Vero E6, CCL-81, Calu-3, Caco-2 cells, anti-HCoV-OC43 Huh-7 cells. We also cellular pharmacology these two compounds Caco-2, Huh-7, 293T, BHK-21, 3T3 human airway epithelial (HAE) cells. Overall, exhibited greater potency...
The present study was undertaken to determine whether asbestos exposure induces the formation of nitric oxide (NO.) radical by rat alveolar macrophages (AM). For this purpose, AM from Sprague-Dawley rats were cultured for 48 h in presence or absence either chrysotile (serpentine) crocidolite (amphibole) fibers. effects fibers compared with those nonfibrogenic carbonyl iron particles. Nitrite (NO2-), stable oxidation product NO. macrophage conditioned medium, assayed Griess reaction....
As the emergence of SARS-CoV-2 variants brings global pandemic to new levels, performance current rapid antigen tests against concern and interest (VOC/I) is significant public health concern. Here, we report assessment Abbot BinaxNOW COVID-19 Antigen Self-Test. Using genetically sequenced remnant clinical samples collected from individuals positive for SARS-CoV-2, assessed that currently pose threats. We measured limit detection various VOC/I in a blinded manner. successfully detected...
Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1 (TEM8/ANTXR1) expression is induced in the vascular compartment of multiple tumors and therefore, a candidate molecule to target tumor therapies. This cell surface mediates anthrax toxin internalization, however, its physiological function blood vessels remains largely unknown. We identified chicken chorioallantoic membrane (CAM) as model system study endogenous TEM8 we found that was transiently between day 10 12 embryonic development,...
Parietal pleural plaques and visceral fibrosis are well-recognized stigmata of occupational asbestos exposure. However, their pathogenesis is poorly understood. Conceivably, phagocytosis fibers by mesothelial cells may stimulate the recruitment fibroblasts to sites asbestos-induced injury. To test this hypothesis, rat parietal were cultured for 6 96 h with or without crocidolite chrysotile (concentration range, 2 100 micrograms/cm2). Asbestos actively phagocytosed incorporated within...
Hepatitis C virus (HCV) nucleoside inhibitors display pan-genotypic activity, a high barrier to the selection of resistant virus, and are some most potent direct-acting agents with durable sustained virologic response in humans. Herein, we report, discovery β-d-2′-Br,2′-F-uridine phosphoramidate diastereomers 27 28, as nontoxic anti-HCV agents. Extensive profiling these two phosphorous was performed select one for in-depth preclinical profiling. The 5′-triphosphate formed from...
To achieve the targeted artificial insemination coverage with current rate of semen production, without affecting conception rate, it needs to reduce number spermatozoa per dose in India as international practice. Therefore, this study was planned perform different levels dilution, compare vitro post-thaw quality and develop a modified low-dose packaging method French mini straw minimise dilution effect. Sixteen ejaculates were collected from Karan Fries bulls (n = 4). The mean percentage...
Viral resistance is a worldwide problem mitigating the effectiveness of antiviral drugs. Mutations in drug-targeting proteins are primary mechanism for emergence drug resistance. It essential to identify mutations elucidate and suggest promising treatment strategies counter However, experimental identification challenging, laborious time-consuming. Hence, effective time-saving computational structure-based approaches predicting high interest discovery research. these dependent on accurate...
Nucleoside analogs are widely used for the treatment of viral diseases (Hepatitis B/C, herpes and human immunodeficiency virus, HIV) various malignancies. ALS-8176, a prodrug 4′-chloromethyl-2′-deoxy-2′-fluoro nucleoside ALS-8112, was evaluated in hospitalized infants respiratory syncytial virus (RSV), but abandoned unclear reasons. Based on structure series novel 4′-modified-2′-deoxy-2′-fluoro nucleosides were synthesized. Newly prepared compounds against RSV, also panel RNA viruses,...
In dairy bovines, estrus detection is the key determinant of herd fertility. However, owing to silent heat in buffaloes, it difficult detect at proper time which turn affects efficiency and reduces conception rate. Further, not clear whether buffaloes during different parities have differential expression behaviour. Therefore, present study was designed behavioural signs expressed by Murrah (n= 94) belonging parities. Estrus done parading teaser bulls (4 times bull a day), expectancy chart...
The present study was conducted to see the impact of weaning ages on post-partum reproductive performance and stress level in buffaloes. Murrah buffaloes (42) were equally randomly divided into three groups viz. group 1, suckling (control); 2, Weaning calves at birth 3, 45th day. Reproductive parameters recorded from calving 90 days post- partum. average first heat artificial insemination (AI) earlier dams 2 than 3 1. However, number service per conception rate better Keeping view early...
This study has shown, for the first time, that in vitro exposure of rat AMs to either crocidolite (amphibole) or chrysotile (serpentine) asbestos fibers induces synthesis not only O2*- anion, but also nitrogen radical, NO*. Furthermore, this asbestos-related effect is enhanced presence interferon-gamma. NO* been implicated several pathologic reactions, such as inflammation and immune complex-mediated cell injury. Additionally, may interact with secondary amines generate nitrosamines, which...
ABSTRACT Interfering with the self-assembly of virus nucleocapsids is a promising approach for development novel antiviral agents. Applied to hepatitis B (HBV), this has led several classes capsid assembly modulators (CAMs) that target by either accelerating nucleocapsid or misdirecting it into non-capsid-like particles. Here, we have assessed structures early intermediates, and without bound CAMs, using molecular dynamics simulations. We find distinct conformations intermediates are induced...