A5100631383- RNA Research and Splicing
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Tuberculosis Research and Epidemiology
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Nuclear Physics and Applications
- Circular RNAs in diseases
- Barrier Structure and Function Studies
- Protein Degradation and Inhibitors
- Pluripotent Stem Cells Research
- FOXO transcription factor regulation
- Alzheimer's disease research and treatments
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Optical Coatings and Gratings
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- RNA regulation and disease
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Plant Molecular Biology Research
University of Chinese Academy of Sciences
2017-2025
Technical Institute of Physics and Chemistry
2025
Chinese Academy of Sciences
2021-2025
The University of Texas MD Anderson Cancer Center
2015-2025
Heilongjiang University of Chinese Medicine
2025
Changchun Institute of Optics, Fine Mechanics and Physics
2025
University of Hong Kong
2025
National Institute of Biological Sciences, Beijing
2024
Anhui University of Traditional Chinese Medicine
2024
Second Hospital of Shandong University
2024
Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) represent two classes of important in eukaryotes. Although these have been implicated organismal development various human diseases, surprisingly little is known about their transcriptional regulation. Recent advances chromatin immunoprecipitation with next-generation DNA sequencing (ChIP-Seq) provided methods detecting transcription factor binding sites (TFBSs) unprecedented sensitivity. In this study, we describe ChIPBase...
Inflammation is a major risk factor for pancreatic ductal adenocarcinoma (PDAC). When occurring in the context of pancreatitis, KRAS mutations accelerate tumor development mouse models. We report that long after its complete resolution, transient inflammatory event primes epithelial cells to subsequent transformation by oncogenic KRAS. Upon recovery from acute inflammation, display an enduring adaptive response associated with sustained transcriptional and epigenetic reprogramming. Such...
Abstract Transcription factors are DNA-binding proteins that have key roles in gene regulation 1,2 . Genome-wide occupancy maps of transcriptional regulators important for understanding and its effects on diverse biological processes 3–6 However, only a minority the more than 1,600 transcription encoded human genome has been assayed. Here we present, as part ENCODE (Encyclopedia DNA Elements) project, data analyses from chromatin immunoprecipitation followed by high-throughput sequencing...
Abstract Microglia are the resident immune cell of central nervous system (CNS), and serve to protect maintain local brain environment. critically dependent on signaling through colony‐stimulating factor 1 receptor (CSF1R); administration CSF1R inhibitors that cross blood barrier (BBB) lead elimination up 99% microglia, depending CNS exposure treatment duration. Once microglia depleted, withdrawal inhibitor stimulates repopulation entire with new cells, conceivably enabling a therapeutic...
Myoblasts are precursor skeletal muscle cells that differentiate into fused, multinucleated myotubes. Current single-cell microfluidic methods not optimized for capturing very large, such as To circumvent the problem, we performed single-nucleus transcriptome analysis. Using immortalized human myoblasts, RNA-seq analysis of single (scRNA-seq) and nuclei (snRNA-seq) found them comparable, with a distinct enrichment long non-coding RNAs (lncRNAs) in snRNA-seq. We then compared snRNA-seq...
Abstract The majority of Alzheimer’s disease (AD) cases are late-onset and occur sporadically, however most mouse models the harbor pathogenic mutations, rendering them better representations familial autosomal-dominant forms disease. Here, we generated knock-in mice that express wildtype human Aβ under control App locus. Remarkably, changing 3 amino acids in sequence to its wild-type counterpart leads age-dependent impairments cognition synaptic plasticity, brain volumetric changes,...
The majority of mammalian genes encode multiple transcript isoforms that result from differential promoter use, changes in exonic splicing, and alternative 3' end choice. Detecting quantifying across tissues, cell types, species has been extremely challenging because transcripts are much longer than the short reads normally used for RNA-seq. By contrast, long-read RNA-seq (LR-RNA-seq) gives complete structure most transcripts. We sequenced 264 LR-RNA-seq PacBio libraries totaling over 1...
Pharmacologic inhibition of C5aR1, a receptor for the complement activation proinflammatory fragment, C5a, suppressed pathology and cognitive deficits in Alzheimer's disease (AD) mouse models. To validate that effect antagonist was specifically via C5aR1 inhibition, mice lacking were generated compared behavior pathology. In addition, since is primarily expressed on cells myeloid lineage, only to lesser extent endothelial neurons brain, gene expression microglia isolated from adult brain at...
Enhancer aberrations are beginning to emerge as a key epigenetic feature of colorectal cancers (CRC), however, comprehensive knowledge chromatin state patterns in tumour progression, heterogeneity these and imparted therapeutic opportunities remain poorly described.We performed epigenomic characterisation by mapping 222 profiles from 69 samples (33 adenocarcinomas, 4 adenomas, 21 matched normal tissues 11 colon cancer cell lines) for six histone modification marks: H3K4me3 Pol II-bound...
The scanning interference field exposure technique is an effective method to fabricate holographic diffraction grating with meter-level size and nano-level precision. main problems of fabricating large-aperture high-precision by this are the displacement measurement stage, control fringe real time compensation phase error. In paper, influence groove error on wavefront aberration analyzed. order improve precision stage range more than one meter, integrated combining sensing laser...
FSHD is characterized by the misexpression of DUX4 in skeletal muscle. Although upregulation thought to be pathogenic cause FSHD, lowly expressed patient samples, and analysis consequences expression has largely relied on artificial overexpression. To better understand native profile its targets, we performed bulk RNA-seq a 6-day differentiation time-course primary FSHD2 myoblasts. We identify set 54 genes upregulated cells, termed FSHD-induced genes. Using single-cell single-nucleus...
A scarcity of functionally validated enhancers in the human genome presents a significant hurdle to understanding how these cis-regulatory elements contribute diseases. We carry out highly multiplexed CRISPR-based perturbation and sequencing identify required for cell proliferation fitness 10 cancer lines. Our results suggest that enhancers, unlike their target genes, display high cell-type specificity chromatin features. They typically adopt modular structure, comprised activating enriched...
Cis-regulatory elements regulate gene expression and lineage specification. However, the potential regulation of cis-elements on mammalian embryogenesis remains largely unexplored. To address this question, we perform single-cell assay for transposase-accessible chromatin using sequencing (ATAC-seq) RNA-seq in embryonic day 7.5 (E7.5) E13.5 mouse embryos. We construct accessibility landscapes with cell spatial information E7.5 embryos, showing patterns distribution potentially functional...
The striped stem borer (SSB), Chilo suppressalis Walker, is a major rice insect pest worldwide. RNA interference (RNAi) has become promising strategy for developing insect-resistant crops. In previous study, five double-stranded RNAs (dsRNAs) targeting important SSB housekeeping genes were overexpressed in rice, but none of the acquired dsRNA-transgenic plants showed significant effects on SSB.Thirteen selected endogenous small RNAs, predicted as novel microRNAs (miRNAs), using artificial...
Microglia, the brain’s resident myeloid cells, play central roles in brain defense, homeostasis, and disease. Using a prolonged colony-stimulating factor 1 receptor inhibitor (CSF1Ri) approach, we report an unprecedented level of microglial depletion establish model system that achieves empty niche adult brain. We identify cell migrates from subventricular zone associated white matter areas. Following CSF1Ri, these amoeboid cells migrate radially tangentially dynamic wave filling distinct...