A5090154188- Zebrafish Biomedical Research Applications
- Genetic and Kidney Cyst Diseases
- Pulmonary Hypertension Research and Treatments
- Adipose Tissue and Metabolism
- Microtubule and mitosis dynamics
- Computational Drug Discovery Methods
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Neonatal and fetal brain pathology
- Peroxisome Proliferator-Activated Receptors
- Congenital heart defects research
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Metabolomics and Mass Spectrometry Studies
- Genetics, Aging, and Longevity in Model Organisms
- Pancreatic function and diabetes
- Hedgehog Signaling Pathway Studies
- Lipid metabolism and biosynthesis
- Angiogenesis and VEGF in Cancer
Mie University
2016-2025
Technology Innovation Institute
2015-2016
Adult Congenital Heart Association
2015
Osaka City University
2015
Shimane University
2015
Keio University
2014
University of California, Los Angeles
2008-2009
Max Planck Research Unit for Neurogenetics
2007
Doshisha University
2007
Center for Autism and Related Disorders
2007
Abstract Background Obesity is a multifactorial disorder influenced by genetic and environmental factors. Animal models of obesity are required to help us understand the signaling pathways underlying this condition. Zebrafish possess many structural functional similarities with humans have been used model various human diseases, including obesity. The purpose study was establish zebrafish diet-induced (DIO). Results were assigned into two dietary groups. One group overfed Artemia (60 mg dry...
Autism is a heterogeneous condition that likely to result from the combined effects of multiple genetic factors interacting with environmental factors. Given its complexity, study autism associated Mendelian single gene disorders or known chromosomal etiologies provides an important perspective. We used microarray analysis compare mRNA expression profile in lymphoblastoid cells males due fragile X mutation (FMR1-FM), 15q11–q13 duplication (dup(15q)), and non-autistic controls. Gene profiles...
Lemon (Citrus limon) contains various bioactive flavonoids and prevents obesity obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects rat model of high-fat diet. To investigate the mechanism action eriocitrin, we conducted feeding experiments zebrafish diet-induced obesity. Oral administration (32 mg/kg/day for 28 days) improved dyslipidaemia decreased lipid droplets liver. DNA...
Abstract Parkin, a ubiquitin E3 ligase of the ring between fingers family, has been implicated in mitochondrial quality control. A series recent reports have suggested that recruitment parkin is regulated by phosphorylation. However, molecular mechanism activates to induce degradation not well understood. Here and contrast previous S-nitrosylation exclusively inhibitory, we identify previously unrecognized site (Cys323) induces degradation. We demonstrate endogenous fact responsible for...
Visceral fat accumulation is one of the most important predictors mortality in obese populations. Administration green tea extract (GTE) can reduce body and risk obesity-related diseases mammals. In this study, we investigated effects mechanisms GTE on adiposity diet-induced (DIO) zebrafish.Zebrafish at 3.5 to 4.5 months post-fertilization were allocated four groups: non-DIO, DIO, DIO + 0.0025%GTE, 0.0050%GTE. The non-DIO group was fed freshly hatched Artemia once daily (5 mg cysts/fish...
Collecting blood from laboratory animals is necessary for a wide variety of scientific studies, but the small size zebrafish makes this common procedure challenging. We developed novel, minimally invasive method to collect repeated samples adult zebrafish. This minimizes trauma and yields low mortality rate 2.3%. The maximum volume that can be collected using technique approximately 2% body weight. To avoid loss anemia hemorrhagic death, we recommend total sample over repeat bleeds should...
Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for from zebrafish has not been established. The previous methods are lethal, such as lateral incision, decapitation and tail ablation. Thus we have developed novel "repeated" method, present here detailed outlining this procedure. This method minimally invasive results in very low mortality rate (2.3%) zebrafish, thus enabling repeated sampling same individual....
The brainstem is a posterior region of the brain, composed three parts, midbrain, pons, and medulla oblongata. It critical in controlling heartbeat, blood pressure, respiration, all which are life-sustaining functions, therefore, damages to or disorders can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate course brain development expected useful for medical research on central nervous system disorders. However, existing organoid models limited extent...
The increasing number of people suffering from metabolic syndrome and obesity is becoming a serious problem not only in developed countries, but also developing countries. However, there are few agents currently approved for the treatment obesity. Those that available mainly appetite suppressants gastrointestinal fat blockers. We have simple rapid method measurement feeding volume Danio rerio (zebrafish). This assay can be used to screen enhancers. In this study, zebrafish were fed viable...
High dietary intake of vegetable products is beneficial against obesity and its related diseases such as dyslipidemia, nonalcoholic fatty liver disease, cancer. We previously developed a diet-induced model zebrafish (DIO-zebrafish) that develops visceral adiposity, steatosis. Zebrafish polyphagous animal; thus we hypothesized DIO-zebrafish could be used for transcriptome analysis anti-obesity effects vegetables.Each exhibited different obesity. focused on "Campari" tomato, which suppressed...
Zebrafish-based chemical screening has recently emerged as a rapid and efficient method to identify important compounds that modulate specific biological processes test the therapeutic efficacy in disease models, including cancer. In leukemia, ablation of leukemia stem cells (LSCs) is necessary permanently eradicate cell population. However, because very small number LSCs populations, their use xenotransplantation studies (in vivo) difficulties functionally pathophysiologically replicating...
Ciliogenesis is generally inhibited in dividing cells, however, it has been unclear which signaling cascades regulate the phenomenon. Here, we report that epidermal growth factor receptor (EGFR) kinase suppresses ciliogenesis by directly phosphorylating deubiquitinase USP8 on Tyr-717 and Tyr-810 RPE1 cells. These phosphorylations elevate activity, then stabilizes trichoplein-Aurora A pathway, an inhibitory mechanism of ciliogenesis. EGFR knockdown serum starvation result through...
Primary cilia play a pivotal role in signal transduction and development are known to serve as signaling hubs. Recent studies have shown that primary cilium dysfunction influences adipogenesis, but the mechanisms unclear. Here, we show mesenchymal progenitors C3H10T1/2 depleted of trichoplein, key regulator formation, significantly longer than control cells fail differentiate into adipocytes. Mechanistically, elongated prevent caveolin-1- and/or GM3-positive lipid rafts from being assembled...
Abstract Background Successful delivery of compounds to the brain and retina is a challenge in development therapeutic drugs imaging agents. This arises because internalization into restricted by blood–brain barrier (BBB) blood-retinal (BRB), respectively. Simple reliable vivo assays are necessary identify that can easily cross BBB BRB. Methods We developed six fluorescent indoline derivatives (IDs) examined their ability BRB zebrafish fluorescence imaging. These IDs were administered live...
Hypertrophic cardiomyopathy (HCM) is characterized by left ventricular hypertrophy and associated with a number of potential outcomes, including impaired diastolic function, heart failure, sudden cardiac death. Various etiologies have been described for HCM, pressure overload mutations in sarcomeric non-sarcomeric genes. However, the molecular pathogenesis HCM remains incompletely understood. In this study, we performed comparative transcriptome analysis to identify dysregulated genes common...