After administration to livestock, a large fraction of antibiotics are excreted unchanged via excreta and can be transferred agricultural land. For effective risk assessment critical factor is determine which expected in the different environmental compartments. excretion, first relevant compartment manure storage. In current study, fate broad scope (n = 46) during storage livestock animals (calves, pigs, broilers) was investigated. Manure samples were fortified with incubated 24 days....

Chloramphenicol (CAP), a broad-spectrum antibiotic, was detected in several herb and grass samples from different geographic origins. Due to its suspected carcinogenicity linkages with the development of aplastic anemia humans, CAP is banned for use food-producing animals European Union (EU) many other countries. However, products animal origin originating Asian countries entering market are still found noncompliant (containing CAP) on regular basis, even when there no history...

Due to the unexpected findings of banned antibiotic chloramphenicol in products animal origin, feed, and straw, hypothesis was studied that drug is naturally present soil, through production by soil bacteria, subsequently can be transferred crops. First, stability studied. The fate highly depends on type showed a half-life approximately one day nonsterile topsoil. It found more stable subsoil sterile soils. Second, studied, it confirmed Streptomyces venezuelae produce at appreciable amounts...

Abstract The stability of an antibiotic is a very important characteristic, especially in the field residue analysis. During method development or validation, has to be demonstrated no matter if used for screening, confirmation, qualitative quantitative A procedure testing antibiotics solutions and food samples using LC-MS/MS described. based on assumption that are stable when stored at −70°C. Representative spiked containing were temperature tested (−18 4°C) After selected storing time...

Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood 1.3 min. The method has been optimized fully validated according forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), 4-fluoroamphetamine (4-FA)....

The extent of unidentified Per- and Poly-fluoroalkyl substances (PFASs) found in environmental samples has led to the development non-targeted screening methods. study presented here reports use liquid chromatography hyphenated with high resolution mass spectrometry detect identify unknown unexpected PFASs by fragment ion flagging (FIF). By exploring all fragmentation spectra for several characteristic fragments including CnF2n+1−, CnF2n-1−, CnF2n-3−, CnF2n-7−, CnF2n-11− CnF2n+1O− presence...

A liquid chromatography-tandem mass spectrometry method for the analysis of seven antiviral drugs, zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine, rimantadine and arbidol, in poultry muscle is reported. The drugs were extracted from homogenized sample using methanol. extract was purified tandem solid-phase extraction combining a cation exchange cartridge phenylboronic acid cartridge. To prevent excessive matrix effects, analytes separated constituents column-switch...

We developed a procedure to determine the "identification power" of an LC-MS/MS method operated in MRM acquisition mode, which is related its selectivity. The probability any compound showing same precursor ion, product ions, and retention time as interest used measure This calculated based upon empirical models constructed from three very large databases. Based final estimation, additional measures assure unambiguous identification can be taken, like selection different or ions. reported...

According to EU legislation a confirmatory method used for residue analysis should be able confirm the identity of compound beyond reasonable doubt. To provide an adequate instrumental set-up, Commission Decision 2002/657/EC introduced concept "identification points". A second aspect assure unequivocal confirmation, is establishment ion ratio and retention time criteria. Currently, gold standard most veterinary drug residues liquid chromatography (LC) coupled tandem mass spectrometry (MS/MS)...

Data on the fate of chemical substances in environment after e.g. manure application is mandatory input for risk assessment perspective a more circular biobased economy. Such studies include persistence study to determine half-life value and mobility study. It recognized that not only native substance should be considered, but also degradation products included might exert similar effect as substance. We report tiered strategy starts with For non-persistent performed if have compound. If so,...

Abstract The transition from linear production towards a circular agro-food system is an important step increasing Europe’s sustainability. This requires re-designing the food systems, which inevitably comes with challenges as regards controlling safety of our food, animals and ecosystem. Where in current systems many hazards are understood well-managed, it anticipated that known may re-emerge new will appear or accumulate, leading to -and less understood- risks. In this perspective paper,...

The use of antibiotics in animal husbandry raises concerns about their environmental impacts, particularly when manure containing antibiotic residues is used as fertilizers. effects on soil nitrogen (N) cycling and nitrous oxide (N2O) emissions remain unclear, especially across different types. We hypothesized that soils with higher adsorption capacities could reduce the bioavailability soil, mitigating impacts N-cycling microbes. To test this, we conducted a 46-day incubation experiment to...

Per- and poly-fluorinated substances (PFASs) are man-made chemicals that have been used for a variety of applications can end up in the food chain. New opinions on risk assessment were recently published by European Food Safety Authority, emphasising need more sensitive methods. From this, minimum required LOQs analytical method analysis milk egg calculated perfluorooctanoic acid (PFOA) GenX (hexafluoropropylene oxide dimer acid, HFPO-DA). A fully validated is described 13 PFASs, including...