A5063069817- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolites in Food Biotechnology
- Biochemical and Structural Characterization
- Synthetic Organic Chemistry Methods
- Enzyme Production and Characterization
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Phytochemical compounds biological activities
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Cocoa and Sweet Potato Agronomy
- Bacterial Genetics and Biotechnology
- Cell Image Analysis Techniques
- Chemical Synthesis and Analysis
- Natural product bioactivities and synthesis
- Food Chemistry and Fat Analysis
- Microbial Community Ecology and Physiology
Fundación Medina
2011-2023
Parque Tecnológico de la Salud
2019
Universidad de Oviedo
2010-2015
Universidad de Granada
2003-2010
Streptomyces albus J1074 is a streptomycete strain widely used as host for expression of secondary metabolite gene clusters. Bioinformatic analysis the genome this organism predicts presence 27 clusters metabolites. We have three different strategies activation some these silent/cryptic in S. J1074: two hybrid polyketide-non-ribosomal peptides (PK-NRP) (antimycin and 6-epi-alteramides), type I PK (candicidin), non-ribosomal (NRP) (indigoidine) glycosylated compounds (paulomycins). By...
Abstract Lantibiotics are ribosomally synthesized and post‐translationally modified peptides (RiPPs) characterized by the presence of lanthionine or methyllanthionine rings their antimicrobial activity. Cacaoidin, a novel glycosylated lantibiotic, was isolated from Streptomyces cacaoi strain fully NMR, mass spectrometry, chemical derivatization approaches genome analysis. The new molecule combines outstanding structural features, such as high number d ‐amino acids, an uncommon tyrosine...
Abstract Streptomyces sp. Tü 6176 produces the cytotoxic benzoxazole nataxazole. Bioinformatic analysis of genome this organism predicts presence 38 putative secondary‐metabolite biosynthesis gene clusters, including those involved in AJI9561 and its derivative nataxazole, antibiotic hygromycin B, ionophores enterobactin coelibactin. The nataxazole cluster was identified characterized: it lacks O‐methyltransferase required to convert into This activity might act as a resistance mechanism,...
The structures of caniferolides A–D have been determined combining NMR and bioinformatics prediction the absolute configuration.
Lanthipeptides are ribosomally synthesized and post-translationally modified peptides characterized by lanthionine (Lan) and/or methyllanthionine (MeLan) residues. Four classes of enzymes have been identified to install these structures in a substrate peptide. Recently, novel class lanthipeptides was discovered that lack genes for known I–IV synthases their biosynthetic gene cluster (BGC). In this study, the dehydration Ser/Thr during biosynthesis V lanthipeptide cacaoidin reconstituted...
Cacaoidin is produced by the strain Streptomyces cacaoi CA-170360 and represents first member of new lanthidin (class V lanthipeptides) RiPP family. In this work, we describe complete identification, cloning heterologous expression cacaoidin biosynthetic gene cluster, which shows unique genes whose functions were not predicted any bioinformatic tool. We also that pathway restricted to strains subspecies subsp. found in public genome databases, where have identified presence other putative...
ABSTRACT Enterococcus faecalis EJ97 produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,327.7 Da). The complete amino acid sequence enterocin was elucidated after automated microsequencing oligopeptides generated by endoproteinase GluC digestion and cyanogen bromide treatment. Transfer the 60-kb conjugative plasmid pEJ97 from bacteriocinogenic strain E. to OG1X conferred production resistance on recipient. genetic determinants were located in an 11.3-kb Eco RI- Bgl II...
Humidimycin (MDN-0010) is a ribosomally synthesized and post-translationally modified peptide (RiPP) belonging to class I lasso peptides, structurally related siamycins, which have been shown strong antimicrobial activities against Gram-positive bacteria possess anti-HIV activity. was isolated from the strain Streptomyces humidus CA-100629, synergize activity of fungal cell wall inhibitor caspofungin. In this work, biosynthetic gene cluster humidimycin identified by genome mining S. cloned...
Enterocin AS-48 production and immunity characters are encoded by 10 genes (as-48ABCC(1)DD(1)EFGH) of the pMB2 plasmid from Enterococcus faecalis S-48 strain. Among these, as-48A, encoding peptide, as-48BC constitute a cluster required for biogenesis full immunity. In this study, levels expression have been altered insertion site-directed mutagenesis as well coupled to trans complementation. Phenotypic studies mutants indicated cotranscription three revealed that inactivation as-48B prevents...
Actinomycetes have been extensively exploited as one of the most prolific secondary metabolite-producer sources and continue to be in focus interest continued search novel bioactive compounds. The availability less expensive next generation genome sequencing techniques has not only confirmed extraordinary richness broad distribution silent natural product biosynthetic gene clusters (BGCs) among these bacterial genomes, but also allowed he incorporation genomics taxonomy systematics. As part...
Among the plethora of unusual secondary metabolites isolated from Stachylidium bicolor are tetrapeptidic endolides A and B. Both tetrapeptides contain 3-(3-furyl)-alanine residues, previously proposed to originate bacterial metabolism. Inspired by this observation, we aimed identify presence endosymbiotic bacteria in S. discover true producer endolides. The endobacterium Burkholderia contaminans was initially detected 16S rRNA gene amplicon sequencing fungal metagenome subsequently isolated....
RES-701-3 and RES-701-4 are two class II lasso peptides originally identified in the fermentation broth of Streptomyces sp. RE-896, which have been described as selective endothelin type B receptor antagonists. These only differ identity C-terminal residue (tryptophan RES-701-3, 7-hydroxy-tryptophan RES-701-4), thus raising an intriguing question about mechanism behind modification tryptophan residue. In this study, we describe identification their biosynthetic gene cluster through genome...
Bacteriocins are antimicrobial peptides produced by bacteria and have a relatively narrow range of activity against closely related strains. AS-48 is circular bacteriocin Enterococcus faecalis that acts many gram-positive some gram-negative bacteria, could well serve as natural food preservative agent. The structure five-helix bundle in which hypothetical plane containing the C(alpha) atoms E4, E20, E49 E58 segregates patch positively charged residues from rest hydrophobic or uncharged...
ABSTRACT Streptomyces lydicus NRRL2433 and S. spectabilis NRRL2494 produce two inhibitors of bacterial RNA polymerase: the 3-acyltetramic acid streptolydigin naphthalenic ansamycin streptovaricin, respectively. Both strains are highly resistant to their own antibiotics. Independent expression rpoB rpoC genes, encoding β- β′-subunits polymerase, respectively, in albus showed that resistance is mediated by , with no effect . Within β-subunit, was confined an amino region harboring “rif...
Streptomyces rimosus ATCC 10970 is the parental strain of industrial strains used for commercial production important antibiotic oxytetracycline. As an actinobacterium with a large linear chromosome containing numerous long repeat regions, high GC content, and single giant plasmid (GLP), these genomes are challenging to assemble. Here, we apply hybrid sequencing approach relying on combination short- long-read next-generation platforms whole-genome restriction analysis by using pulsed-field...
Four AS-48 mutants (Trp24Ala, Gly13Lys, Leu40Lys and Ala53Ser) were obtained by site-directed mutagenesis. The minimal inhibitory concentration of each peptide showed that only residue Trp24 was unquestionably involved in the biological activity. Guanidine hydrochloride-induced unfolding assays a three-state transition denaturation process, suggesting molten-globule-like conformation after first transition.
Despite the availability of many culture-based antibiotic screening methods, lack sensitive automated methods to identify functional molecules directly from microbial cells still limits search for new biologically active compounds. The effectiveness detection is influenced by solubility assayed compounds, indicator strain sensitivity, culture media and assay configuration. We describe a qualitative high throughput system detecting cell-perturbing bacterial colonies employing two opposed agar...
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Pentaminomycins F-H (1-3), a group of three new hydroxyarginine-containing cyclic pentapeptides, were isolated from cultures Streptomyces cacaoi subsp. strain along with the known pentaminomycins A-E. The structures peptides determined by combination mass spectrometry, NMR, and Marfey's analyses. F (1) G (2) shown to contain rare amino acid 3-(2-pyridyl)-alanine. This finding represents first reported examples nonribosomal containing this residue. ldlld chiral sequence found for compounds...
Bacteriocins belong to the wide variety of antimicrobial ribosomal peptides synthesised by bacteria. Enterococci are Gram-positive, catalase-negative bacteria that produce lactic acid as major end product glucose fermentation. Many enterococcal strains bacteriocins, named enterocins. We describe in this work, structural characterisation 44 residues-long enterocin EJ97, produced Enterococcus faecalis EJ97. To end, we have used a combined theoretical and experimental approach. First,...
Abstract Lantibiotics are ribosomally synthesized and post‐translationally modified peptides (RiPPs) characterized by the presence of lanthionine or methyllanthionine rings their antimicrobial activity. Cacaoidin, a novel glycosylated lantibiotic, was isolated from Streptomyces cacaoi strain fully NMR, mass spectrometry, chemical derivatization approaches genome analysis. The new molecule combines outstanding structural features, such as high number d ‐amino acids, an uncommon tyrosine...