A5024054429- Lipid Membrane Structure and Behavior
- Plant Disease Resistance and Genetics
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Nanopore and Nanochannel Transport Studies
- Biological Activity of Diterpenoids and Biflavonoids
- RNA and protein synthesis mechanisms
- Plant Genetic and Mutation Studies
- Gut microbiota and health
- Bioactive Compounds and Antitumor Agents
- Circular RNAs in diseases
- Force Microscopy Techniques and Applications
- Protein Structure and Dynamics
- Probiotics and Fermented Foods
- Evolution and Genetic Dynamics
- Helicobacter pylori-related gastroenterology studies
- Electrostatics and Colloid Interactions
- Natural Compound Pharmacology Studies
- Machine Learning in Bioinformatics
- Anaerobic Digestion and Biogas Production
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
Huazhong Agricultural University
2018-2024
Guizhou Normal University
2023-2024
University of Science and Technology of China
2023
Jilin University
2023
Qilu University of Technology
2022
First Affiliated Hospital of Zhengzhou University
2021
Zhengzhou University
2020-2021
Henan Provincial Center for Disease Control and Prevention
2021
National Clinical Research Center for Digestive Diseases
2021
Center of Hubei Cooperative Innovation for Emissions Trading System
2018
Abstract Although major advances in genomics have initiated an exciting new era of research, a lack information regarding cis -regulatory elements has limited the genetic improvement or manipulation pigs as meat source and biomedical model. Here, we systematically characterize their functions 12 diverse tissues from four pig breeds by adopting similar strategies ENCODE Roadmap Epigenomics projects, which include RNA-seq, ATAC-seq, ChIP-seq. In total, generate 199 datasets identify more than...
We calculate the Gaussian curvature modulus kappa of a systematically coarse-grained (CG) one-component lipid membrane by applying method recently proposed Hu et al. [Biophys. J., 2012, 102, 1403] to MARTINI representation 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). find value kappa/kappa = -1.04 +/- 0.03 for elastic ratio between and mean deduce kappa(m)/kappa(m) -0.98 0.09 monolayer ratio, where latter is based on plausible assumptions distance z0 neutral surface from bilayer...
The force needed to buckle a thin elastic surface is proportional its bending rigidity. This fact suggests using buckling setup measure the modulus of lipid membranes. Extending work Noguchi [Phys. Rev. E 83, 061919 (2011)10.1103/PhysRevE.83.061919], we systematically derive highly accurate analytical expressions for forces along and perpendicular buckle, elucidate some their counterintuitive properties framework stress tensor. Furthermore, estimate corrections due thermal fluctuations find...
Taxifolin is a natural antioxidant polyphenol with various bioactivities and has many beneficial effects on human gut health. However, little known of its function colitis. In this study, the protective taxifolin colitis symptoms, inflammation, signaling pathways, colon microbiota were investigated using dextran sulfate sodium (DSS)-induced mice. Intriguingly, pre-administration alleviated symptoms histological changes DSS-challenged Supplementation significantly inhibited secretions tumor...
Inflammatory bowel disease (IBD) is a chronic intestinal disorder threatening human health. Di-peptide alanyl-glutamine (Ala-Gln) has various beneficial effects on gut However, its role and functional mechanism in treating IBD are still not clear. Therefore, the protective of Ala-Gln glutamine (Gln) dextran sulfate sodium- (DSS-) induced colitic mice were investigated this study. The results showed that oral supplementation or Gln significantly attenuated colitis symptoms mice, including...
Feed efficiency (FE) is one of the main factors that determine production costs in pig industry. In this study, RNA Sequencing (RNA-seq) was applied to identify genes and long intergenic non-coding RNAs (lincRNAs) are differentially expressed (DE) adipose tissues Yorkshire pigs with extremely high low FE. total, 147 annotated 18 lincRNAs were identified as DE between high- low-FE pigs. Seventeen significantly correlated 112 at transcriptional level. Gene ontology (GO) analysis revealed...
N6-Methyladenosine (m6A) refers to the methylation modification occurring at nitrogen-6 position of adenosine. Many human physiological processes such as modulation spermatogenesis are caused by m6A RNA modifications. However, relationship between regulators and kidney renal clear cell carcinoma (KIRC) remains rarely investigated. This work aimed explore influence in KIRC. We examined abnormally expressed among different clinicopathological features recognized three subgroups (KIRC1, KIRC2,...
We present an experimental study on the natural sloshing frequencies and damping ratios of a liquid-elastic plate coupled system. Two types edges including complete clamped combined (two free two clamped) are examined. The measured experimentally agree well with theoretical predictions. Our findings indicate that tend to increase number other parameters fixed, while ratio exhibits opposite trend. Despite general agreement, there still minor deviations between analytical solutions...
DDO-6101, a simplified structure obtained from the Garcinia natural product (NP) gambogic acid (GA), has been previously shown to possess high cytotoxicity variety of human tumour cell lines. To improve its physicochemical properties and in vivo cytotoxic potency, series novel carbamate-bearing derivatives based on DDO-6101 was synthesized characterized. The structural modifications revealed that presence carbamate moiety useful for obtaining comparable improved aqueous solubility...
Feed efficiency (FE) is an important trait affecting costs in swine industry. Investigation on FE-related genes different tissues valuable for molecular breeding. Hypothalamus a convergent and integrated centre multiple nutrient-related signals. The present study identified 363 differentially expressed (DE) 14 DE lincRNAs the hypothalamus of high- low-FE Yorkshire pigs. Furthermore, 983 significantly correlated gene-lincRNA pairs were through weighted correlation network analysis (WGCNA)...
We propose the design for a nanoparticle carrier that combines three existing motifs into single construct: liposome is stabilized by anchoring it to an enclosed solid core via extended polymeric tethers are chemically grafted and physisorb surrounding lipid membrane. Such would exhibit several enticing properties, among them: (i) stabilizes against variety of external stresses, while preserving aqueous compartment between membrane; (ii) interplay parameters such as polymer length or...
Diabetic nephropathy is a lethal disease that can lead to chronic kidney and end-stage disease. Exosomes, which are nanosized extracellular vesicles, closely involved in intercellular communication. Most importantly, exosomes play critical roles occurrence development. However, the function of diabetic progression has not been fully elucidated. In present study, we determined expression profiles differences lncRNAs, mRNAs, circRNAs miRNAs derived from human renal tubular epithelial cells...
Abstract Background Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered subset of domain-inlaid Nme2Cas9 editors to bring the deaminase domain closer nontarget strand improve efficiency. Results Our results demonstrated that Nme2ABE8e-797 inserted between amino acids 797 798 significantly increased...
miRNAs reportedly participate in various biological processes, such as skeletal muscle proliferation and differentiation. However, the regulation of differentially expressed (DE) their function myogenesis remain unclear. Herein, miRNA expression profiles during C2C12 differentiation were analyzed relation to chromatin states by RNA-seq, ATAC-seq, ChIP-seq. We identified 19 known nine novel at days 0, 1, 2, 4. The was related 113 surrounding open regions defined ATAC-seq peaks. Of these...
Abstract Objective This study aims to clarify the mechanisms underlying transcriptional regulation and regulatory roles of lncRNAs in skeletal muscle cell differentiation. Methods We analysed expression patterns via time‐course RNA‐seq. Then, we further combined ATAC‐seq ChIP‐seq investigate governing differentially expressed (DE) lncRNAs. Weighted correlation network analysis GO were conducted identify transcription factor (TF)‐lncRNA pairs related Results identified 385 DE during C2C12...
Although large-scale and accurate identification of cis-regulatory elements on pig protein-coding long non-coding genes has been reported, similar study miRNAs is still lacking. Here, we systematically characterized the in muscle fat by adopting miRNAomes, ChIP-seq, ATAC-seq, RNA-seq Hi-C data. In total, 257 (85.95%) expressed including 226 known 31 novel were identified. Especially, associated with super-enhancers, active promoters, "A" compartment significantly higher than those typical...