A5113603025- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- Transgenic Plants and Applications
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
Henan Normal University
2024
Henan Province Water Conservancy Survey and Design Research
2024
City of Hope
2002-2007
City Of Hope National Medical Center
2004-2007
Beckman Research Institute
2003-2007
Institute of Molecular Biology and Biophysics
1999
Whitney Museum of American Art
1997-1998
Yale University
1997-1998
Combinatorial therapies for the treatment of HIV-1 infection have proven to be effective in reducing patient viral loads and slowing progression AIDS. We developed a series RNA-based inhibitors use gene therapy-based infection. The transcriptional units been inserted into backbone replication-defective lentiviral vector capable transducing wide array cell types, including CD34+ hematopoietic progenitor cells. combinatorial therapeutic RNA harbors U6 Pol III promoter-driven short hairpin...
The phenomenon of RNA interference mediated by small interfering RNAs (siRNAs) is a potent gene-silencing mechanism. A number recent studies demonstrated inhibition HIV-1 replication in cultured cells using this approach. To make further progress and harness technology for gene therapy stem cell setting, vivo primary hematopoietic are needed. Using an HIV-based lentiviral vector we introduced anti-Rev siRNA construct into CD34+ progenitor cells. siRNA-transduced were allowed to mature...
A primary advantage of lentiviral vectors is their ability to pass through the nuclear envelope into cell nucleus thereby allowing transduction nondividing cells. Using HIV-based vectors, we delivered an anti-CCR5 ribozyme (CCR5RZ), a nucleolar localizing TAR RNA decoy, or Pol III-expressed siRNA genes cultured and The CCR5RZ driven by adenoviral VA1 III promoter, while human U6 snRNA III-transcribed decoy embedded in U16 snoRNA (designated U16TAR), siRNAs were expressed from promoter....
Gene therapeutic strategies show promise in controlling human immunodeficiency virus (HIV) infection and restoring immunological function. A number of efficacious anti-HIV gene constructs have been described so far, including small interfering RNAs (siRNAs), RNA decoys, transdominant proteins, ribozymes, each with a different mode action. However, as HIV is prone to generating escape mutants, the use single construct would not be adequate afford long range-viral protection. On this basis,...
LR1 is a B cell-specific, sequence-specific DNA binding activity that regulates transcription in activated cells. also binds Ig heavy chain switch region sequences and may function class recombination. contains two polypeptides, of 106 kDa 45 kDa, here we report the 106-kDa component nucleolin. This identification, initially made by microsequence analysis, was verified showing ( i ) LR1–DNA increased cells transfected with nucleolin cDNA expression construct; ii recognized antibodies raised...
ABSTRACT We demonstrate a novel approach for coexpression of short hairpin RNA (shRNA) with an open reading frame which exploits transcriptional read-through minimal polyadenylation signal from Pol II promoter. first observed efficient inducible expression enhanced green fluorescent protein along anti-rev shRNA. took advantage this observation to test the transdominant negative mutant (humanized) human immunodeficiency type 1 (HIV-1) Rev (huRevM10) shRNA via HIV-1-inducible fusion The and...
Aim: The domain of exosomal circRNAs has seen rapid growth over the past few years, yet a thorough synthesis scholarly contributions been lacking. This study employs bibliometric analysis to uncover focal points and trends in circRNA research. Methods: We selected articles on from Web Science Core Collection (WoSCC) deployed VOSviewer for co-authorship co-occurrence analysis. CiteSpace was tapped calculate citation bursts documents keywords, generating time-zone map. Additionally,...
The hnRNP D protein interacts with nucleic acids bothin vivoandin vitro.Like many other proteins that interact RNA, it contains RBD (or "RRM") domains and arg-gly-gly (RGG) motifs. We have examined the organization localization of human murine genes encode protein. Comparison predicted sequences in mouse shows they are 96.9% identical (98.9% similar). This very high level conservation suggests a critical function for D. Sequence analysis humanHNRPDgene is encoded by eight exons two...
To understand the regulation of enzymes that carry out immunoglobulin heavy chain class switch recombination, we have assayed recombination extrachromosomal substrates carrying region sequences in cell lines representing different stages lymphoid development. Both pre-B and mature B supported substrate but derived from later development did not. Recombination not occur an erythroid or a macrophage line. Most junctions recovered transfection cells mapped to heterogeneous sites within Sμ Sγ...
Lipopolysaccharide (LPS) is a B cell mitogen which can stimulate murine primary cells to proliferate and carry out immunoglobulin heavy chain class switch recombination. LPS also function as an endotoxin, may cause DNA damage apoptosis in certain types of cells. We have previously reported that LPS-activated contain nuclear foci stain brightly with anti-Rad51 antibodies (Liet al.(1996)Proc. Natl. Acad. Sci. USA93, 10222–10227). now analyzed Rad51 induced both immortalized by treatment the...
EBV is strongly associated with Burkitt's lymphoma, a B cell malignancy. In certain types of the c-myc gene has undergone translocation to S regions heavy chain switch recombination. It not been established whether infection induces recombination activities, which in turn promote c-myc, or precedes viral and provides growth advantage that further enhanced by factors encoded induced virus. To distinguish between these possibilities, we have compared level activities EBV-negative BJAB, its...
To investigate the potential role of epiphytic biofilms in inhibition submerged macrophytes on Microcystis aeruginosa, we compared effect crude extracts from Myriophyllum spicatum growth and photosynthesis pigments M. aeruginosa under conditions retaining removing biofilms. The results showed that there was a significant enhancement inhibitory effects plant against when By end experiment, rate reached up to 85.6% for extract equivalent fresh weight 10.0 g·L-1 within groups containing...