A5107905532- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- Phytochemical compounds biological activities
- Mineral Processing and Grinding
- Calcium signaling and nucleotide metabolism
- Image Processing and 3D Reconstruction
- Cancer Immunotherapy and Biomarkers
- Complement system in diseases
- Osteoarthritis Treatment and Mechanisms
- Blood groups and transfusion
- Microbial Metabolic Engineering and Bioproduction
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Immunology Research
- Bacterial Genetics and Biotechnology
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Metabolomics and Mass Spectrometry Studies
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2025
Shandong University of Traditional Chinese Medicine
2025
First Affiliated Hospital of GuangXi Medical University
2024
Guangxi Medical University
2024
Harbin Medical University
2024
Chongqing University
2024
Qingdao Municipal Hospital
2024
Qingdao University
2024
Indiana University School of Medicine
2022
Xiamen University
2022
Precise genome editing via homology-directed repair (HDR) after double-stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise for gene therapy. However, HDR efficiency remains low in some cell types, including of great clinical interest, such as human induced pluripotent stem cells (iPSCs).Here, we show that a double cut donor, which is flanked by single guide RNA (sgRNA)-PAM sequences released CRISPR/Cas9 cleavage, increases twofold to fivefold relative...
One-step multiple gene disruption in the model organism Saccharomyces cerevisiae is a highly useful tool for both basic and applied research, but it remains challenge. Here, we report rapid, efficient, potentially scalable strategy based on type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated proteins (Cas) system to generate disruptions simultaneously S. cerevisiae. A 100 bp dsDNA mutagenizing homologous recombination donor inserted between two direct...
Genome editing of human induced pluripotent stem cells (iPSCs) is instrumental for functional genomics, disease modeling, and regenerative medicine. However, low efficiency has hampered the applications CRISPR-Cas9 technology in creating knockin (KI) or knockout (KO) iPSC lines, which largely due to massive cell death after electroporation with plasmids. Here, we report that transient delivery BCL-XL increases survival by ∼10-fold plasmid transfection, leading a 20- 100-fold increase...
Abstract Aims A mutation in the phospholamban (PLN) gene, leading to deletion of Arg14 (R14del), has been associated with malignant arrhythmias and ventricular dilation. Identifying pre-symptomatic carriers vulnerable myocardium is crucial because arrhythmia can result sudden cardiac death, especially young adults PLN-R14del mutation. This study aimed at assessing efficiency efficacy vivo genome editing, using CRISPR/Cas9 a cardiotropic adeno-associated virus-9 (AAV9), improving function...
Purpose The coagulation process and infiltration of macrophages affect the progression prognosis lung adenocarcinoma (LUAD) patients. This study was designed to explore novel classification methods that better guide precise treatment LUAD patients on basis macrophages. Methods Weighted gene coexpression network analysis (WGCNA) applied identify M2 macrophage-related genes, TAM marker genes were acquired through scRNA-seq data. MSigDB KEGG databases used obtain coagulation-associated genes....
Lung adenocarcinoma (LUAD) is a very common and lethal kind of lung malignancy. An increasing number studies indicated that tissue-resident memory T (TRM) cells played significant roles in anti-cancer immunity. In our previous study, EXO1 was found to be core gene for TRM the prognosis LUAD. However, tumor microenvironment, its application diagnosis prediction LUAD are still inadequately explored. this RNA expression, DNA methylation, CNV, somatic mutation data EXO1, corresponding patients'...
Abstract The bone marrow (BM) niche regulates multiple hematopoietic stem cell (HSC) processes. Clinical treatment for hematological malignancies by HSC transplantation often requires preconditioning via total body irradiation, which severely and irreversibly impairs the BM regeneration. Novel strategies are needed to enhance regeneration in irradiated BM. We compared effects of EGF, FGF2, PDGFB on using human mesenchymal cells (MSCs) that were transduced with these factors lentiviral...
Aim: The domain of exosomal circRNAs has seen rapid growth over the past few years, yet a thorough synthesis scholarly contributions been lacking. This study employs bibliometric analysis to uncover focal points and trends in circRNA research. Methods: We selected articles on from Web Science Core Collection (WoSCC) deployed VOSviewer for co-authorship co-occurrence analysis. CiteSpace was tapped calculate citation bursts documents keywords, generating time-zone map. Additionally,...
We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use optimized CRISPR-Cas9 together with a double-cut donor increases homology-directed repair-mediated precise gene knockin efficiency by 5- to 10-fold. Here, report integration cell reprogramming and genome editing in single step. found expression Cas9 KLF4 using significantly...
Background: PANoptosis plays an important role in many inflammatory diseases. However, there are no reports on the association between and CD. Materials Methods: This study used five machine learning algorithms - least absolute shrinkage selection operator, support vector machine, random forest, decision tree Gaussian mixture models to construct CD’s signature. Unsupervised hierarchical clustering analysis was identify PANoptosis-associated subgroups of Gene Ontology (GO) enrichment...
Familial exudative vitreoretinopathy (FEVR) is an autosomal dominant genetic disease. An induced pluripotent stem cell line (EHTJUi002-A) was generated from umbilical cord blood mononuclear cells (UCBMCs) of a neonate with heterozygous mutation p.W226X(c.678G>A) in the FZD4 gene. This iPSC model offers very valuable resource to study pathological mechanism FEVR vitro.
<p>Sensitivity and apoptotic response to BET PROTACs in SKM1, MV4:11, LNCaP OVCAR8 cells.</p>
<p>Supplemental Table 1: TaqMan CNV assays; Supplemental 2:Sanger PCR primers</p>
<p>Sensitivity and apoptotic response to BET PROTACs in SKM1, MV4:11, LNCaP OVCAR8 cells.</p>
Abstract Background The inducible expression system plays an important role in engineering Escherichia coli for chemical production, however, it still heavily relies on expensive inducers, like IPTG. There is a pressing need to develop alternative systems with more affordable inducers. Results We herein reported copper-induced gradient E. based the two-component Cus and T7 RNA polymerase (RNAP). By integrating gene encoding RNAP at CusC locus, we managed program eGFP under promoter response...