A5022019637- Hippo pathway signaling and YAP/TAZ
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Retinal Diseases and Treatments
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Retinal Imaging and Analysis
- Osteoarthritis Treatment and Mechanisms
- Cardiac Fibrosis and Remodeling
- TGF-β signaling in diseases
- Immune responses and vaccinations
- Inflammatory mediators and NSAID effects
- Birth, Development, and Health
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Cancer-related gene regulation
- Blood properties and coagulation
- PI3K/AKT/mTOR signaling in cancer
- Sphingolipid Metabolism and Signaling
- Blood Coagulation and Thrombosis Mechanisms
- Bone Metabolism and Diseases
Tianjin Medical University
2016-2025
Second Hospital of Tianjin Medical University
2022-2025
Peking University First Hospital
2025
Peking University
2025
Tianjin Medical University Eye Hospital
2018-2023
Institute of Hematology & Blood Diseases Hospital
2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Tianjin University of Traditional Chinese Medicine
2018
Institute of Genetics and Developmental Biology
2018
Chinese Academy of Sciences
2018
Local flow patterns determine the uneven distribution of atherosclerotic lesions. This research aims to elucidate mechanism regulation nuclear translocation Yes-associated protein (YAP) under oscillatory shear stress (OSS) in atheroprone phenotype endothelial cells (ECs). We report here that OSS led tyrosine phosphorylation and strong, continuous YAP ECs is dependent on integrin α5β1 activation. overexpression blunted anti-atheroprone effect an α5β1–blocking peptide (ATN161) Apoe–/– mice....
Angiogenesis is a complex process regulating endothelial cell (EC) functions. Emerging lines of evidence support that YAP (Yes-associated protein) plays an important role in the angiogenic activity ECs.The objective this study was to specify effect EC on angiogenesis and its underlying mechanisms.In ECs, vascular growth factor reduced phosphorylation time dose dependently increased nuclear accumulation. Using Tie2Cre-mediated transgenic mice, we found promoted postnatal retina tumor tissues....
The Hippo/Yes-associated protein (YAP) pathway has pivotal roles in innate immune responses against pathogens macrophages. However, the role of YAP macrophages during atherosclerosis and its mechanism activation remain unknown. Here, we find that overexpression myeloid cells aggravates atherosclerotic lesion size infiltration macrophages, whereas deficiency reduces plaque. Tumor necrosis factor receptor-associated 6 (TRAF6), a downstream effector interleukin-1β (IL-1β), triggers...
Atherosclerosis preferentially occurs at specific sites of the vasculature where endothelial cells (ECs) are exposed to disturbed blood flow. Translocation integrin α5 lipid rafts promotes activation and ligation, which is critical for oscillatory shear stress (OSS)-induced EC activation. However, underlying mechanism OSS promoted raft translocation has remained largely unknown.The objective this study was specify mechanotransduction OSS-induced subsequent activation.Mass spectrometry...
Background: Vascular endothelial cells are critical for maintaining blood pressure (BP) by releasing biologically active molecules, such as nitric oxide. A non-endothelial cell resident matricellular protein, COMP (cartilage oligomeric matrix protein), plays a pivotal role in cardiovascular homeostasis, but little is known about its regulatory effect on BP. Methods: Mice were infused with AngII (angiotensin II; 450 ng/kg per minute) 3 days via an osmotic minipump, and BP was monitored...
Rationale: Timely inhibition of inflammation and initiation resolution are important to repair injured tissues. MST1/2 (mammalian STE20-like protein kinase 1/2) acts as a regulator macrophage-associated immune responses bacterial infections. However, the role in regulating macrophage phenotype function myocardial infarction (MI) remains unclear. Objective: To determine underlying mechanism cardiac post-MI. Methods Results: Using LysMCre -mediated Mst1/2 -deficient mice, we found that MST1...
Background: Atherosclerosis occurs mainly at arterial branching points exposed to disturbed blood flow. How MST1 (mammalian sterile 20-like kinase 1), the primary in mechanosensitive Hippo pathway modulates flow induced endothelial cells (ECs) activation and atherosclerosis remains unclear. Methods: To assess role of vivo, mice with EC-specific Mst1 deficiency on ApoE −/− background ( iECKO ) were used an model generated by carotid artery ligation. Mass spectrometry, immunoprecipitation,...
Endothelial cells regulate vascular tone by producing both relaxing and contracting factors to control the local blood flow. Hypertension is a common side effect of mTORC1 (mammalian target rapamycin complex 1) inhibitors. However, role endothelial in hypertension remains elusive. The present study aimed determine Ang II (angiotensin II)-induced underlying mechanism. activity was increased vitro vivo. Blood pressure higher Tie-2-Cre-mediated regulatory associated protein mTOR rapamycin;...
BACKGROUND: Immune cells are closely associated with all processes of cardiac repair after myocardial infarction (MI), including the initiation, development, and resolution inflammation. Spleen extramedullary hematopoiesis (EMH) serves as a crucial source emergency mature blood that generated through self-renewal differentiation hematopoietic stem/progenitor (HSPCs). However, how EMH responds to MI role in remains unclear. METHODS: To assess spleen MI, Tcf21 CreER Scf flox/flox mouse model...
Endothelial cells (EC) play essential roles in retinal vascular homeostasis. This study aimed to characterize EC heterogeneity and functional diversity using single-cell RNA sequencing. Systematic analysis of cellular compositions cell-cell interaction networks identified a unique cluster with high inflammatory gene expression diabetic retina; sphingolipid metabolism is prominent aspect correlated changes function. Among sphingolipid-related genes, alkaline ceramidase 2 (ACER2) showed the...
Abstract Endothelial NO synthase (eNOS) expression is regulated by a number of transcriptional and post-transcriptional mechanisms, but the effects competing endogenous RNAs (ceRNAs) on eNOS mRNA underlying mechanisms are still unknown. Our bioinformatic analysis revealed three highly expressed eNOS-targeting miRNAs (miR-15b, miR-16, miR-30b) in human endothelial cells (ECs). Among 1103 targets these miRNAs, 15 mRNAs share common disease association with eNOS. Gene correlation patients...
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic disorder worldwide. Multiple metabolic disorders, such as hyperlipidemia, hyperglycemia, insulin resistance and obesity, have been reportedly associated with NAFLD, but little known about detailed mechanisms.Here, we explored effects of multiple especially hyperglycemia on lipid accumulation in using several well-established animal models. We found that deposition was increased both type 1 diabetes high-fat diet (HFD)...
The accurate electrochemical model is of great significance for the simulation and design fuel cell power systems. In order to estimate parameters proton exchange membrane (PEMFC) model, a modified multi-group DNA genetic algorithm (MMDNA-GA) which inspired by mechanism biological proposed. MMDNA-GA, three new crossover operators adaptive mutation are developed improving global searching ability. To enhance population diversity overcome premature convergence algorithm, inter-generational...
<p dir="ltr"><b>Abstract</b></p><p dir="ltr"><a href="" target="_blank">Endothelial cells (EC) play essential roles in retinal vascular homeostasis. This study aimed to characterize EC heterogeneity and functional diversity using single-cell RNA sequencing. Systematic analysis of cellular compositions cell-cell interaction networks identified a unique cluster with high inflammatory gene expression diabetic retina; sphingolipid metabolism is prominent...
<p dir="ltr"><b>Abstract</b></p><p dir="ltr"><a href="" target="_blank">Endothelial cells (EC) play essential roles in retinal vascular homeostasis. This study aimed to characterize EC heterogeneity and functional diversity using single-cell RNA sequencing. Systematic analysis of cellular compositions cell-cell interaction networks identified a unique cluster with high inflammatory gene expression diabetic retina; sphingolipid metabolism is prominent...