A5101670917- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Sulfur Compounds in Biology
- Magnetism in coordination complexes
- Free Radicals and Antioxidants
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Redox biology and oxidative stress
- Molecular Sensors and Ion Detection
- Advanced MRI Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Respiratory Support and Mechanisms
- Carbon and Quantum Dots Applications
- Inflammatory Biomarkers in Disease Prognosis
- Cerebrospinal fluid and hydrocephalus
- Nerve injury and regeneration
- Hormonal and reproductive studies
- Wound Healing and Treatments
- GABA and Rice Research
- Fungal Infections and Studies
- Erythrocyte Function and Pathophysiology
- Ultrasound in Clinical Applications
- CRISPR and Genetic Engineering
- Cardiac Ischemia and Reperfusion
Sun Yat-sen University
2022-2025
Fifth Affiliated Hospital of Sun Yat-sen University
2022-2025
Jiaxing University
2022-2025
Tianjin Medical University
2015-2024
Jiangsu University
2023
First Hospital of Jiaxing
2022
Second Artillery General Hospital of Chinese People's Liberation Army
2013
Shenzhen Children's Hospital
2011-2012
Abstract Nanomaterials with enzyme‐mimetic activities are possible alternatives to natural enzymes. Mimicking enzymatic enantioselectivity remains a great challenge. Herein, we report that cysteine‐derived chiral carbon dots (CDs) can mimic topoisomerase I mediate topological rearrangement of supercoiled DNA enantioselectively. d ‐CDs more effectively catalyze the transition plasmid from nicked open‐circular configuration than l ‐CDs. Experiments suggest underlying mechanism: intercalatively...
Double-electron electron resonance (DEER) can be used to track the structural dynamics of proteins in their native environment, cell. This method provides distance distribution between two spin labels attached at specific, well-defined positions a protein. For viable under in-cell conditions, label and its attachment protein should exhibit high chemical stability Here we present low-temperature, trityl–trityl DEER measurements on model proteins, PpiB (prolyl cis–trans isomerase from E. coli)...
Background: Vascular endothelial cells are critical for maintaining blood pressure (BP) by releasing biologically active molecules, such as nitric oxide. A non-endothelial cell resident matricellular protein, COMP (cartilage oligomeric matrix protein), plays a pivotal role in cardiovascular homeostasis, but little is known about its regulatory effect on BP. Methods: Mice were infused with AngII (angiotensin II; 450 ng/kg per minute) 3 days via an osmotic minipump, and BP was monitored...
Triarylmethyl (TAM or trityl) radicals are becoming important for measuring distances in proteins and nucleic acids. Here, we report on a new trityl spin label CT02MA, which conjugates to protein via redox stable thioether bond. The performance of the was demonstrated W-band double electron-electron resonance (DEER) distance measurements doubly trityl-labelled mutants immunoglobulin G-binding 1 (GB1) ubiquitin. For both CT02MA-labelled measured, by applying chirped pump pulse(s), relatively...
Abstract Nanomaterials with enzyme‐mimetic activities are possible alternatives to natural enzymes. Mimicking enzymatic enantioselectivity remains a great challenge. Herein, we report that cysteine‐derived chiral carbon dots (CDs) can mimic topoisomerase I mediate topological rearrangement of supercoiled DNA enantioselectively. d ‐CDs more effectively catalyze the transition plasmid from nicked open‐circular configuration than l ‐CDs. Experiments suggest underlying mechanism: intercalatively...
Purpose: Chronic obstructive pulmonary disease (COPD) concurrent with respiratory failure (RF) is devastating, and may result in death disability.Systemic immune-inflammation index (SII) a new prognostic biomarker linked to unfavorable outcomes of acute coronary syndrome, ischemic stroke, heart failure.Nonetheless, its role COPD rarely investigated.Consequently, this study intends assess the accuracy SII predicting prognosis COPD.Patients Methods: The clinical information was retrospectively...
Abstract Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse roles. Thus, analytical techniques that can detect, quantify, distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection quantitation of biothiols, including up to three different a single sample, using electron paramagnetic resonance (EPR) spectroscopy trityl‐radical‐based probe (MTST). We term this...
The poor clinical situation of type 2 diabetes-induced erectile dysfunction (T2DMED) creates an urgent need for new therapeutic targets. To reveal the conserved molecular mechanism T2DMED across species. rat and mouse models were constructed to extract mRNA from corpus cavernosum high-throughput sequencing. differentially expressed genes (DEGs) analyzed Kyoto Encyclopedia Genes Genomes (KEGG), Gene Ontology (GO), Protein-Protein Interaction Networks performed by bioinformatics methods....
The Triglyceride-Glucose (TyG) index, a reliable marker for insulin resistance, is now employed to assess the onset and prognosis of various conditions like acute coronary syndrome, chronic kidney disease, ischemic stroke. However, whether TyG index can be used respiratory failure (RF) risk among Chronic obstructive pulmonary disease (COPD) patients remains uncertain. present study aims delve into link between RF in COPD patients. Individuals with were retrospectively acquired from MIMIC-IV...
Tetrathiatriarylmethyl (TAM, trityl) radicals have attracted considerable attention as spin probes for biological electron paramagnetic resonance (EPR) spectroscopy and imaging owing to their sharp EPR singlet signals high biostability. However, in vivo applications were limited by the short blood circulation lifetimes strong binding with albumins. Our previous results showed that PEGylation is a feasible method overcome issues facing of TAM radicals. In present study, we synthesized series...
FLOURY ENDOSPERM 2 (FLO2), encoding a tetratricopeptide repeat domain (TPR)-containing protein located in the nucleus, is considered to be regulatory that controls biosynthesis of seed storage substances. The diversity flo2 allele attributable for variations grain appearance, amylose content (AC), and physicochemical properties, influencing eating cooking quality (ECQ) rice. In this study, we used CRISPR/Cas9 introduce loss-of-function mutations into gene Suken118 (SK118), widely cultivated...
Abstract Tetrathiatriarylmethyl (TAM, trityl) radicals have found wide applications as spin probes/labels for EPR spectroscopy and imaging, polarizing agents dynamic nuclear polarization. The high hydrophilicity of TAM is essential their biomedical applications. However, the synthesis hydrophilic (e.g., OX063) extremely challenging has only been reported in patent literature, to date. Herein, an efficient a highly water‐soluble radical...
Pulsed dipolar electron paramagnetic resonance (PD-EPR) measurement is a powerful technique for characterizing the interactions and conformational changes of biomolecules. The extraction these distance restraints from PD-EPR experiments relies on manipulation spin–spin pairs. orthogonal spin labeling approach offers unique advantages by providing multiple distances between different Here, we report an efficient based exploiting cyclization 1,2-aminothiol moiety in protein (e.g., N-terminal...
A novel mitochondria-targeted superoxide-responsive nitric oxide donor was developed by incorporation of diphenylphosphinyl and triphenylphosphonium groups into diazeniumdiolate, enabling remarkable protection against ischemia/reperfusion injury in H9c2 cells isolated rat hearts.
Tetrathiatriaylmethyl (trityl) radicals have found wide biomedical applications as magnetic resonance probes. Trityl and their derivatives are generally stable toward biological reducing agents such glutathione (GSH) ascorbate. We demonstrate that the triester (ET-03) triamide (AT-03) of Finland trityl radical exhibit unique reduction by thiols GSH cysteine (Cys) to generate corresponding carbanions evidenced loss EPR signal appearance characteristic UV–vis absorbance at 644 nm under...
Tetrathiatriarylmethyl (trityl) radicals have been recently shown to react with biological oxidoreductants including glutathione (GSH), ascorbic acid (Asc), and superoxide anion radical (O2•–). However, how the substituents affect reactivity of trityl is still unknown. In this work, five asymmetric were synthesized their reactivities GSH, Asc, O2•– investigated. Under aerobic conditions, GSH induces fast decays for thioether- (TSA) N-methyleneglycine-substituted (TGA) derivatives slow decay...
Abstract EPR spectroscopy, coupled with the use of tetrathiatriarylmethyl (TAM) radicals, has been a reliable method to detect superoxide radical (O 2 .− ). However, specificity and biocompatibility TAM radicals need be further improved. Although derivatization may overcome drawbacks current esterification or amidation through carboxylic groups greatly changes their redox properties makes them inert O . Herein, synthesis perthiatriarylmethyl (PST) its dendritic derivatives, PST‐TA PST‐NA, in...
Tetrathiatriarylmethyl (TAM, trityl) radicals have found wide applications in electron paramagnetic resonance (EPR) oximetry. However, the biomedical of TAM were exclusively limited to an extracellular region owing their negatively charged nature. The intracellular delivery still remains a challenge. In present work, we report liposome-based method encapsulate water-soluble Finland trityl radical CT-03 for its delivery. Using thin lipid film hydration method, CT-03-loaded liposomes prepared...
Supramolecular host-guest interactions of trityl-nitroxide (TN) biradicals CT02-VT, CT02-AT and CT02-GT with methyl-β-cyclodextrin (M-β-CD), hydroxypropyl-β-cyclodextrin (H-β-CD) γ-cyclodextrin (γ-CD) were investigated by EPR spectroscopy. In the presence cyclodextrins (i.e., γ-CD, M-β-CD H-β-CD), complexes CT02-VT are formed where nitroxide linker parts possibly interact cyclodextrins' cavities. Complexation leads to suppression intramolecular through-space spin-spin exchange coupling in...
Abstract Background This study aims to construct a model predicting the probability of RF in AECOPD patients upon hospital admission. Methods retrospectively extracted data from MIMIC-IV database, ultimately including 3776 patients. The were randomly divided into training set ( n = 2643) and validation 1133) 7:3 ratio. First, LASSO regression analysis was used optimize variable selection by running tenfold k-cyclic coordinate descent. Subsequently, multifactorial Cox employed establish...
Abstract Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse roles. Thus, analytical techniques that can detect, quantify, distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection quantitation of biothiols, including up to three different a single sample, using electron paramagnetic resonance (EPR) spectroscopy trityl‐radical‐based probe (MTST). We term this...