A5001549014- Sexual function and dysfunction studies
- Hormonal and reproductive studies
- Sperm and Testicular Function
- RNA modifications and cancer
- Urinary Bladder and Prostate Research
- Sexuality, Behavior, and Technology
- Cancer-related molecular mechanisms research
- Testicular diseases and treatments
- Genital Health and Disease
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sarcoma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Pelvic floor disorders treatments
- Urologic and reproductive health conditions
- Chemotherapy-induced organ toxicity mitigation
- Ubiquitin and proteasome pathways
- Adenosine and Purinergic Signaling
- Autophagy in Disease and Therapy
- Sexual Differentiation and Disorders
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- Inflammatory Biomarkers in Disease Prognosis
Sun Yat-sen University
2018-2025
Fifth Affiliated Hospital of Sun Yat-sen University
2018-2025
Sichuan University
2025
West China Second University Hospital of Sichuan University
2025
South China Normal University
2024
Heilongjiang Bayi Agricultural University
2024
Chongqing Medical University
2024
Children's Hospital of Chongqing Medical University
2024
University of California, San Francisco
2024
Min Sheng General Hospital
2023
Abstract The corpus cavernosum is the most important structure for penile erection, and its dysfunction causes many physiological psychological problems. However, cellular heterogeneity signalling networks at molecular level are poorly understood because of limited access to samples. Here, we profile 64,993 human cavernosal single-cell transcriptomes from three males with normal erection five organic erectile patients. Cell communication analysis reveals that fibroblasts central paracrine...
Abstract Testicular germ cell tumours (TGCTs) rank as the most common malignancy in men aged 20‐34 years, and seminomas are type of TGCTs. As a crucial anti‐tumour agent with explicit toxicity, cisplatin may render resistance through intertwined mechanisms, even disease entities high curative ratio, such seminoma. Previously, we established cisplatin‐resistant seminoma TCam‐2 (TCam‐2/CDDP) cells showed that epigenetic regulations, non‐coding RNA (ncRNA) interactions, might orchestrate fate...
Cisplatin is an efficient chemotherapeutic agent for various solid tumors, but its usage restricted by nephrotoxicity. A single dose of cisplatin can cause acute kidney injury (AKI), which characterized rapid reduction in function. However, the current therapies, such as hydration, are limited. It vital to develop novel therapeutic reagents that have both anticancer and renoprotective properties. The objective this study was determine whether ammonium tetrathiomolybdate (TM), a copper...
Abstract Background The development of single‐cell sequencing technology has expanded the understanding cell heterogeneity and disease progression in male genitourinary system. However, complex processing unprofessional analytical annotations limit daily use widely sharing published datasets. Objectives Single‐cell data male‐specific tissues organs. Materials methods were downloaded from studies processed based on Seurat R package, including quality control, clustering, reduction graph...
Clinical treatment of erectile dysfunction (ED) caused by cavernous nerve (CN) injury during pelvic surgery is difficult. Low-intensity pulsed ultrasound (LIPUS) can be a potential strategy for neurogenic ED (NED). However, whether Schwann cells (SCs) respond to LIPUS stimulation signals unclear. This study aims elucidate the signal transmission between SCs paracrine exosome (Exo) and neurons stimulated LIPUS, as well analyze role mechanisms exosomes in CN repair after injury.The major...
Priapism is a condition of persistent penile erection in the absence sexual excitation. Of men with sickle cell disease (SCD), 40% display priapism. The disorder dangerous and urgent condition, given its association fibrosis eventual erectile dysfunction. Current strategies to prevent progression are poor because lack fundamental understanding molecular mechanisms for Here we demonstrate that increased adenosine novel causative factor contributing two independent animal models priapism,...
The adenosine A2b receptor (A2bR) was considered to play an oncogenic role in many human malignancies. However, the expression and molecular function of A2bR bladder urothelial carcinoma (BUC) have not been well elucidated. Herein, we found that higher than other receptors BUC tissues cells, it upregulated compared with matched normal tissues. Furthermore, high associated poor prognosis patients BUC. In addition, suppression inhibited proliferation, migration invasion cells arrested cell...
BackgroundProstate adenocarcinoma (AdPC) cells can undergo lineage switching to neuroendocrine and develop into therapy-resistant prostate cancer (NEPC). While genomic/epigenetic alterations are shown induce differentiation via an intermediate stem-like state, RNA splicing factor SRRM4 transform AdPC NEPC xenografts through a direct transdifferentiation mechanism. Whether also regulate stem-cell gene network for development remains unclear.MethodsMultiple cell models were transduced by...
MicroRNA (miRNA)-gene interactions are well-recognized as involved in the progression of almost all cancer types including prostate cancer, which is one most common cancers men. This study explored significantly dysregulated genes and miRNAs elucidated potential miRNA-gene regulatory network cancer. Integrative analysis normal transcriptomic data The Cancer Genome Atlas dataset was conducted using both differential expression weighted correlation (WGCNA). Thirteen (RRM2, ORC6, CDC45, CDKN2A,...
Our previous work shows Autophagy enhanced resistance to cisplatin in seminoma. The expression of the N6-methyladenosine (m6A) methyltransferases METTL3 was significantly increased cisplatin-resistant TCam-2 cell line We aimed investigate role m6A methylation autophagy and chemosensitivity seminoma cells.Plasmid siRNA were used overexpress knockdown METTL3. detected by western blot immunofluorescence, respectively. downstream targets quantitative real-time PCR (qRT-PCR) blot, level them...
Clear cell renal carcinoma (ccRCC) is closely associated with steroid hormones and their receptors affected by lipid metabolism. Recently, there has been growing interest in the carcinogenic role of NR3C1, sole gene responsible for encoding glucocorticoid receptor. However, specific NR3C1 ccRCC remains unclear. The present study was thus developed to explore underlying mechanism NR3C1's effects ccRCC.Expression verified various tumor databases assessed using RT-qPCR western blot. Stable...
The simplified International Index of Erectile Function (IIEF-5) is a convenient, reliable and validated diagnostic tool for erectile dysfunction (ED). However, few studies focused on IIEF-5 in ED patients with different pathophysiological causes. ,We aim to compare the score among specific pathophysiologies this study.The 3,327 (median age 39 years) was analyzed. primary causes were determined by comprehensive procedures urology/andrology clinics five training hospitals. Patients uncertain...
Abstract The role of TDRG1 in tumorigenesis and the progression seminoma, as well its regulating chemosensitivity seminoma to cisplatin through PI3K/Akt/mTOR signaling pathway, has been previously defined. However, detailed mechanism underlying expression concomitant chemoresistance conditions are unknown. Furthermore, it reported that non‐protein‐coding RNAs play an important a variety vital processes including cellular chemosensitivity. remains In this study, using microarray analysis, we...
The adenosine A1 receptor (A1R) has been reported to be involved in the pathogenesis of various cancers, and effects A1R on different cancers are pleiotropic. However, role renal cell carcinoma (RCC) remains not well-known.The expression RCC cells was detected by quantitative real-time PCR Western blotting analysis. Cell proliferation using an MTT assay a colony formation assay. Tumor growth also evaluated nude mice. invasion migration were wound healing transwell cycle distribution...
We previously identified TDRG1 (testis developmental related gene 1), a novel with exclusive expression in testis, promoted the proliferation and progression of cultured human seminoma cells through PI3K/Akt/mTOR signaling. As increasing evidence reveal that aberrant activation this signaling is involved cisplatin resistance. Then, study, we further explored whether regulated chemosensitivity TCam-2 to cisplatin. Our researches showed could regulate viability following treatment vitro...
This study was aimed to evaluate the potential function of circ-0001946 in progression colorectal cancer (CRC) and related regulatory mechanism. First, expression levels circRNA_0001946 microRNA-135a-5p (miR-135a-5p) normal CRC tissues were measured by quantitative real-time polymerase chain reaction (RT-qPCR). In addition, cell proliferation assessed Cell Counting Kit-8 (CCK-8) assay, migration invasion evaluated Transwell assays, cycle patterns determined flow cytometry. The relationship...
Anacardic acid (AA) is a mixture of 2-hydroxy-6-alkylbenzoic homologs. It widely regarded as non-specific histone acetyltransferase inhibitor p300. The effects and the mechanisms AA in LNCaP cells (prostate cancer cells) remain unknown. To investigate effect on cells, we had carried out several experiments found that inhibits cell proliferation, induces G1/S cycle arrest apoptosis cell. via which acts may be due to following aspects. First, can regulate p300 transcription protein level...
We aimed to investigate the effect and mechanism of icariin on male sexual function. Forty-eight Crl:CD1(ICR) mice were randomly divided into control, low-, medium- high-dose group (intragastric administration 50, 100 200 mg/kg/d for 21 days). Mating experiment was then performed at a ratio 1: 3 (male: female). The mating behaviours recorded. genital indexes serum testosterone, nitric oxide (NO), hypothalamic dopamine (DA) 5- hydroxy tryptophan (5-HT) concentrations measured. expression...
Alzheimer's disease (AD) is a neurodegenerative in humans. The accumulation of amyloid-β (Aβ) plays critical role the pathogenesis AD. Previous studies indicated that Salvianolic acid B (SalB) could ameliorate Aβ-induced memory impairment. However, whether SalB influence generation Aβ unclear. Here, we show (25, 50, or 100 µM) reduces Aβ40 and Aβ42 culture media by decreasing protein expressions BACE1 sAPPβ SH-SY5Y-APPsw cells. Meanwhile, increases levels ADAM10 sAPPα has no impact on APP...