A5034350830- Immune Cell Function and Interaction
- Trypanosoma species research and implications
- Traditional and Medicinal Uses of Annonaceae
- Research on Leishmaniasis Studies
- Hematopoietic Stem Cell Transplantation
- Phytochemistry and Biological Activities
- Phytochemical compounds biological activities
- Natural product bioactivities and synthesis
- T-cell and B-cell Immunology
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Malaria Research and Control
- Toxin Mechanisms and Immunotoxins
- CAR-T cell therapy research
- Insect and Pesticide Research
- Traditional Chinese Medicine Analysis
- Neuroblastoma Research and Treatments
- Platelet Disorders and Treatments
- Histiocytic Disorders and Treatments
- Immunotherapy and Immune Responses
- Identification and Quantification in Food
- IL-33, ST2, and ILC Pathways
- Phytochemistry and Bioactivity Studies
- Herbal Medicine Research Studies
Linköping University
2023
Goethe University Frankfurt
2010-2022
University Hospital Frankfurt
2004-2022
Medizinische Hochschule Hannover
2021
Klinik und Poliklinik für Kinder- und Jugendmedizin
2019
Pierre Fabre (Germany)
2017
University of Freiburg
2014-2017
The University of Sydney
2016
Deutsches Diabetes-Zentrum e.V.
2015
Swiss Tropical and Public Health Institute
2009-2014
Abstract Cancers arising from germline DNA mismatch repair deficiency or polymerase proofreading (MMRD and PPD) in children harbour the highest mutational microsatellite insertion–deletion (MS-indel) burden humans. MMRD PPD cancers are commonly lethal due to inherent resistance chemo-irradiation. Although immune checkpoint inhibitors (ICIs) have failed benefit previous studies, we hypothesized that hypermutation caused by will improve outcomes following ICI treatment these patients. Using an...
MYH9-related disease (MYH9-RD) is a rare autosomal-dominant disorder caused by mutations in the gene for nonmuscle myosin heavy chain IIA (NMMHC-IIA). MYH9-RD characterized considerable variability clinical evolution: patients present at birth with only thrombocytopenia, but some of them subsequently develop sensorineural deafness, cataract, and/or nephropathy often leading to end-stage renal (ESRD). We searched genotype-phenotype correlations largest series consecutive collected so far (255...
Allogeneic Natural Killer (NK) cells are used for adoptive immunotherapy after stem cell transplantation. In order to overcome technical limitations in NK purification and activation, the following study investigates impact of different variables on recovery, cytotoxicity T depletion during GMP-grade selection. 40 products were derived from 54 unstimulated donor leukaphereses using immunomagnetic CD3 T-cell depletion, followed by a CD56 enrichment step. For either 2.1 program single or...
To characterize natural killer (NK) cell subpopulations during activation, we analyzed the NK receptor repertoire and functionality of purified clinical scale CD56+CD3− donor cells stimulation with 1000 U/mL interleukin (IL)-2 for up to 14 days. In a phase I/II trial, investigated efficacy feasibility nonidentical infusion in patients neuroblastoma after haploidentical stem transplantation. After IL-2 stimulation, large differences distribution CD16negative CD16positive were found 12 donors....
In a screen of 880 extracts from plants and fungi for antiplasmodial, antitrypanosomal, leishmanicidal activity, an ethyl acetate extract the mushroom Ganoderma lucidum showed antiplasmodial activity with 79% inhibition at 4.9 μg/mL. HPLC-based profiling subsequent isolation compounds yielded seven lanostanes (1−7), which three (2, 3, 7) were new. A new benzofuran derivative (8) farnesyl hydroquinone ganomycin B was also identified. The structures relative configurations elucidated by...
Allogeneic natural killer (NK) cells are known to show medium high cytotoxic activity against HLA-nonidentical leukemia or tumor cells. For a possible benefit of post transplant treatment with NK after haploidentical stem cell transplantation (haplo-SCT) we developed clinical scale procedure for processing observing Good Manufacturing Practice (GMP).Allogeneic donor were selected from 15 unstimulated leukaphereses using two rounds immunomagnetic T depletion, followed by an enrichment step....
Abstract Background: Normal values of lymphocyte subpopulations for healthy children and adults have been published in defined age groups exclusively, which results difficult data interpretation patients close to the limit contiguous group ranges. In addition, normal a number not established date. Objective: The aim this study was develop model provides continuous age‐dependent reference values. This applied such as naïve memory T cells well their activation profile with diagnostic relevance...
A screen of 1800 plant and fungal extracts with subsequent HPLC-based activity profiling was done to identify new antiprotozoal leads from nature. This led the identification cynaropicrin (1) herb Centaurea salmantica L. (Asteraceae) as a potent in vitro inhibitor Trypanosoma brucei rhodesiense. It preferentially inhibited T. b. rhodesiense (IC50 0.3 µM) gambiense 0.2 µM), compared cruzi 4.4 Plasmodium falciparum 3.0 µM). Testing against melarsoprol- pentamidine-resistant strains (IC50s µM...
In a screening of Iranian plants for antiprotozoal activity, an <i>n</i>-hexane extract the roots <i>Salvia sahendica</i> potently inhibited growth <i>Plasmodium falciparum</i> K1 strain. Subsequent HPLC-based activity profiling led to identification seven known and one new abietane-type diterpenoid. Structure elucidation was achieved by analysis spectroscopic data including 1D 2D NMR. The absolute configuration sahandol (<b>7</b>) sahandone (<b>8</b>) were assigned comparison experimental...
Constitutional mismatch repair deficiency (CMMRD) is caused by germline pathogenic variants in both alleles of a gene. Patients have an exceptionally high risk numerous pediatric malignancies and benefit from surveillance adjusted treatment. The diversity its manifestation, ambiguous genotyping results, particularly PMS2, can complicate diagnosis preclude timely patient management. Assessment low-level microsatellite instability nonneoplastic tissues detect CMMRD, but current techniques are...
<b>Objectives:</b> Regulatory T cells (Tregs) exert their anti-inflammatory activity predominantly by cell contact-dependent mechanisms. A study was undertaken to investigate the regulatory capacity of autologous peripheral blood Tregs in contact with synovial tissue cultures, and evaluate presence blood, fluid patients rheumatoid arthritis (RA). <b>Methods:</b> 44 RA 5 osteoarthritis were included study. The frequency interferon (IFN)γ-secreting quantified CD3-depleted cultures co-cultured...
Hydrangenone, a new heptacyclic isoprenoid with 6/7/6/5/5 membered carbon ring skeleton, was isolated from the aerial parts of Salvia hydrangea. The structure established by extensive NMR spectroscopic methods. relative and absolute configuration 1 assigned NOESY X-ray crystallographic analysis comparison experimental calculated electronic circular dichroism (ECD) spectra. Compound showed in vitro antiplasmodial activity, an IC50 value 1.4 μM against P. falciparum. A plausible biosynthetic...
Salvadione C (1) and perovskone B (2), two new triterpenoids with rare carbon skeletons, were isolated from an antiplasmodial n-hexane extract of Salvia hydrangea. The absolute configuration was determined by comparison experimental calculated electronic circular dichroism (ECD) spectra. In vitro activity against Plasmodium falciparum K1 strain, Trypanosoma brucei rhodesiense STIB 900 cytotoxicity in rat myoblast (L6) cells determined. Compounds 1 2 showed activity, IC50 values 1.43 0.18 μM...
Two hundred and seven extracts were prepared from sixty plants South Africa screened for <i>in vitro</i> activity against <i>Trypanosoma brucei rhodesiense, Trypanosoma cruzi, Leishmania donovani</i>, <i>Plasmodium falciparum</i>. For the 21 which inhibited growth of one or more parasites with than 95 % at 10 µg/mL, IC<sub>50</sub> values all four protozoal cytotoxic L6 myoblasts determined. Amongst most notable results are activities <i>Psoralea pinnata</i> (IC<sub>50</sub> 0.15 µg/mL),...
Background & AimsConstitutional mismatch repair deficiency (CMMRD) is a rare recessive childhood cancer predisposition syndrome caused by germline variants. Constitutional microsatellite instability (cMSI) CMMRD diagnostic hallmark and may associate with risk. We quantified cMSI in large patient cohort to explore genotype–phenotype correlations using novel MSI markers selected for blood.MethodsThree CMMRD, 1 Lynch syndrome, 2 control blood samples were genome sequenced >120× depth. A pilot...