A5077283974- PI3K/AKT/mTOR signaling in cancer
- Indoor and Outdoor Localization Technologies
- Quinazolinone synthesis and applications
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Ginseng Biological Effects and Applications
- Cancer Mechanisms and Therapy
- Milk Quality and Mastitis in Dairy Cows
- PARP inhibition in cancer therapy
- Growth Hormone and Insulin-like Growth Factors
- Genetic and phenotypic traits in livestock
- Animal Behavior and Welfare Studies
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Animal Disease Management and Epidemiology
- Speech and Audio Processing
- Effects of Environmental Stressors on Livestock
- Mobile Crowdsensing and Crowdsourcing
- Animal Nutrition and Physiology
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- Biochemical and Molecular Research
- Probiotics and Fermented Foods
- Synthesis and biological activity
- Underwater Vehicles and Communication Systems
Xichang University
2024-2025
New Hope Liuhe (China)
2021-2024
Nanjing Agricultural University
2024
Huawei Technologies (China)
2022-2023
University of Cambridge
2017-2022
Zhejiang University
2015-2020
IBM Research (China)
2020
Aurora St. Luke's Medical Center
2012
Counseling Center
2012
Capital Normal University
2010
Abstract Purpose: To evaluate the preclinical pharmacokinetics and antitumor efficacy of a novel orally bioavailable poly(ADP-ribose) polymerase (PARP) inhibitor, ABT-888. Experimental Design: In vitro potency was determined in PARP-1 PARP-2 enzyme assay. vivo evaluated syngeneic xenograft models combination with temozolomide, platinums, cyclophosphamide, ionizing radiation. Results: ABT-888 is potent inhibitor both Kis 5.2 2.9 nmol/L, respectively. The compound has good oral bioavailability...
The Akt kinases are central nodes in signal transduction pathways that important for cellular transformation and tumor progression. We report the development of a series potent selective indazole-pyridine based inhibitors. These compounds, exemplified by A-443654 (K(i) = 160 pmol/L versus Akt1), inhibit Akt-dependent cells vivo dose-responsive manner. In vivo, inhibitors slow progression tumors when used as monotherapy or combination with paclitaxel rapamycin. Tumor growth inhibition was...
Abstract Polo-like kinase 1 (Plk1) is required for multiple stages of mitosis and up-regulated in many human malignancies. We depleted Plk1 expression using small interfering RNA (siRNA) showed defects bipolar spindle formation cytokinesis, growth inhibition, apoptosis induction cancer cell lines. To our surprise, depletion normal cells did not result obvious cycle defects, induce significant inhibition at least two cycles. In addition, siRNA inhibited colony soft agar tumorigenesis a HT1080...
Pim-1, Pim-2, and Pim-3 are a family of serine/threonine kinases which have been found to be overexpressed in variety hematopoietic malignancies solid tumors. Benzothienopyrimidinones were discovered as novel class Pim inhibitors that potently inhibit all three with subnanomolar low single-digit nanomolar Ki values exhibit excellent selectivity against panel diverse kinases. Protein crystal structures the bound Pim-1 complexes benzothienopyrimidinones 3b (PDB code 3JYA), 6e 3JYO), 12b 3JXW)...
Poly(ADP-ribose) polymerase (PARP) senses DNA breaks and facilitates repair via the polyADP-ribosylation of various binding proteins. We explored mechanism potentiation temozolomide cytotoxicity by PARP inhibitor ABT-888. showed that cells treated with need to be exposed ABT-888 for at least 17 24 hours achieve maximal cytotoxicity. The extent correlates level double-stranded as indicated gammaH2AX levels. In synchronized cells, damaging in presence during S phase generated high levels...
Growth hormone (GH) initiates its cellular action by properly dimerizing GH receptor (GHR). A substantial fraction of circulating is complexed with a high-affinity GH-binding protein (GHBP) that in many species can be generated GHR proteolysis and shedding the receptor's ligand-binding extracellular domain. We previously showed this 1) acutely promoted phorbol ester 12-myristate 13-acetate (PMA), 2) requires metalloprotease activity, 3) generates both shed GHBP membrane-associated...
Insulin is important for maintaining the responsiveness of liver to growth hormone (GH). deficiency results in a decrease GH receptor (GHR) expression, which can be reversed by insulin administration. In osteoblasts, continuous treatment decreases fraction cellular GHR localized plasma membrane. Thus, it not clear whether hyperinsulinemia an enhancement or inhibition action. We asked stimulation, similar what occurs hyperinsulinemic states, resistance. Our present studies suggest that...
Compound 7 was identified as a potent (IC50 = 14 nM), selective, and orally bioavailable (F 70% in mouse) inhibitor of protein kinase B/Akt. While promising efficacy observed vivo, this compound showed effects on depolarization Purkinje fibers an vitro assay CV hypotension vivo. Guided by X-ray structure bound to A, which has 80% homology with Akt the domain, our efforts have focused structure-activity relationship (SAR) studies phenyl moiety, attempt address cardiovascular liability further...
Growth hormone receptor (GHR) is a cytokine superfamily member that binds growth (GH) via its extracellular domain and signals interaction of cytoplasmic with JAK2 other signaling molecules. GHR target for inducible metalloprotease-mediated cleavage in perimembranous domain, process liberates the as soluble GH-binding protein leaves behind cell-associated remnant containing transmembrane domains. metalloproteolysis can be catalyzed by tumor necrosis factor-α-converting enzyme (ADAM-17)...
(1) propose an evaluation indicator of the fattening efficiency commercial pigs (Yorkshire × Landrace Duroc)-fattening index (FEI). (2) Analyze correlation to find main productive factors affecting FEI. (3) Compare and analyze yearly/monthly/different piglets' sources performance in 2020 2021. The data included 2592 pig batches 3266 2021, with a total 6,134,234 pigs. Descriptive statistics difference analysis were carried out on 16 whole year single/multiple for two consecutive years. same...
Abstract Background Porcine Epidemic Diarrhea (PED) is a highly contagious disease caused by Virus (PEDV), resulting in mortality rate of suckling piglets as high 100%. Vaccination the primary strategy for controlling PEDV infection, however, there currently lack reliable methods assessing efficacy vaccination. This study aimed to analyze serum and colostrum samples from 75 parturient sows with specific vaccination measure levels IgG, IgA, neutralizing antibodies (nAbs) against PEDV,...
ABSTRACT The E2 protein of papillomaviruses is a site-specific DNA binding nuclear protein. It functions as the primary replication origin recognition and assists in assembly preinitiation complex. also helps regulate transcription from native viral promoter. consists an amino-terminal (N) trans -acting domain, central hinge (H) carboxyl-terminal (C) dimerization domain. highly divergent among papillomaviruses, little known about its functions. We fused enhanced green fluorescent (GFP) with...
GH signaling depends on functional interaction of the receptor (GHR) and cytoplasmic tyrosine kinase, Janus kinase 2 (JAK2), which possesses a C-terminal domain, catalytically inactive pseudokinase domain just N-terminal to an half shown by us others harbor elements for GHR association. Computational analyses indicate that JAKs contain in their N termini ( approximately 450 residues) divergent FERM domains. domains (or subdomains within them) JAKS may be important associations with cytokine...
We have designed peptide inhibitors that potently inhibit Akt both in vitro and inside cells. These are selective for versus other closely related kinases. The peptides the phosphorylation of a biotinylated Bad by with potency up to 100 nM. shown binding between Akt1 these requires MgATP. Mutating two putative sites Ala (nonsubstrate) increases inhibitory while mutating aspartic acid (phosphorylation mimetic) reduces potency. When delivered into cells, can cellular activity cell growth....
Pan-cancer analysis provides valuable insights into common and distinct molecular mechanisms across various cancer types. Co-expression networks, which captured the cordinated expression of genes proteins, offer potentials for identifying robust biomarkers targeted therapies. However, current-existing co-expression construction method involves either RNA or Protein data in isolation, limiting our understanding on multi-perspective interactions cancer. To address this gap, we integerated both...
The HADHB gene encodes the beta-subunit of 3-hydroxy acyl-CoA dehydrogenase, closely related to energy metabolism, fatty acid synthesis, and catabolism. This study aimed investigate effect on proliferation differentiation bovine preadipocytes gain new insights into mechanisms adipocyte regulation. RNA was extracted from adipose tissue yellow cattle CDS region cloned. Meanwhile, isolation cultivation were used for siRNA adenovirus overexpression, quantitative real-time PCR, transcriptome...
The objective of this study was to assess the effect fermented onion (FO) on growth performance Liangshan black sheep (LBS) and elucidate its potential molecular mechanisms from a multi-omics perspective. A total 20 LBS were randomly assigned one four groups fed diets containing 0, 10, 20%, or 30% onions, respectively. initial final body weights recorded. Following termination experiment, control group exhibiting most significant increase in average daily gain (ADG) selected for slaughter....
Mono- and disaccharide-containing glycopolymers were synthesized by cyanoxyl-mediated polymerization of acrylamide with acrylate-derivatized mono- disaccharides. We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin heparan sulfate as molecular chaperone for fibroblast growth factor-2 (FGF-2). Specifically, compound was identified protects FGF-2 from proteolytic, acid, heat-induced degradation, while selectively promoting factor...
Mastitis is considered the most significant and persistent disease in dairy cows, bringing about large economic losses. Subclinical mastitis brings major cost implications, for it difficult to detect due absence of any visible indications can persist mammary tissue throughout lactation. Immunomodulators have been widely used reduce intramammary infections by modulating bovine gland. Atractylodis macrocephalae Koidz. polysaccharides (RAMP), extracted from herbal medicine, has especially its...