Abstract Plasmids are widely used gene vectors in therapy, yet their efficient delivery remains a major challenge for achieving optimal therapeutic outcomes. Recently, poly(β‐amino esters) (PBAEs) have emerged as promising carriers non‐viral due to tunable structures and high efficiency. Nonetheless, the cationic nature of PBAEs raises toxicity concerns, lack tissue‐specific targeting capability limits clinical application. Herein, novel PBAE plasmid is constructed, which contains disulfide...

Random-pattern skin flaps are commonly used and valuable tools in reconstructive surgery, however, post-operative random flap necrosis remains a major common complication. Previous studies have suggested that activating autophagy, pathway for degradation of intracellular waste, may improve survival. In this study, we investigated whether trehalose, novel potent autophagy activator, improves viability. Our results demonstrated trehalose significantly viability, augments blood flow, decreases...

Osteoarthritis (OA) is a serious and frequently occurring disease in the elderly, characterized by cartilage degeneration proliferation of bone structure.

Spinal cord ischemia-reperfusion injury (SCIRI) is the main complication after repair of a complex thoracoabdominal aortic aneurysm. Many clinical treatments are not ideal due to pathophysiological process this injury. Baicalein (BA), component derived from roots herb Scutellaria baicalensis, may contribute successful treatment ischemia/reperfusion injury.In present study, effects BA on spinal and underlying mechanisms were assessed.Spinal ischemia was induced in C57BL/6 mice by blocking...

In plastic and reconstructive surgery, random-skin flaps replantation have been commonly used to repair skin defects. However, flap necrosis due ischemia IR injury, limit clinical applications. Betulinic acid (BA), a pentacyclic triterpene of plant origin, may facilitate the survival flaps. present study, effects BA on underlying mechanisms were assessed.Forty-eight mice with "McFarlane flap" model randomly divided into Control, groups BA+3MA groups, which administrated vehicle BA, 3MA BA....

Worldwide, osteoarthritis (OA) is regarded as the most widespread, distressing, and limiting chronic disease that affects degenerative joints. Currently, there no treatment available to modify progression of OA. The pathogenesis OA significantly linked with oxidative stress pyroptosis. Astaxanthin (Ast) a natural ketocarotenoid pigment potent antioxidant activity shown effectively alleviate cartilage damage in However, its bioavailability greatly limited due poor water solubility, high...

Abstract Random‐pattern skin flaps are widely applied to rebuild and restore soft‐tissue damage in reconstructive surgery; however, ischemia subsequent ischemia‐reperfusion injury lead flap necrosis major complications. Exenatide, a glucagon‐like peptide‐1 analog, exerts therapeutic benefits for diabetic wounds, cardiac injury, nonalcoholic fatty liver disease. Furthermore, Exenatide is known activator of autophagy, which complex process subcellular degradation that may enhance the viability...

Pan-cancer analysis provides valuable insights into common and distinct molecular mechanisms across various cancer types. Co-expression networks, which captured the cordinated expression of genes proteins, offer potentials for identifying robust biomarkers targeted therapies. However, current-existing co-expression construction method involves either RNA or Protein data in isolation, limiting our understanding on multi-perspective interactions cancer. To address this gap, we integerated both...

Multiterritory perforator flap survival is commonly applied in surgical tissue reconstructions and covering of large skin defects. However, multiple risk factors such as ischemia, reperfusion injury apoptosis after reconstructive surgeries cause necrosis distal parts with outcomes ranging from poor aesthetic appearance to failure. A few studies have reported that sitagliptin promotes angiogenesis inhibits apoptosis. little known about sitagliptin-induced autophagy especially on the model....

Random skin flaps are commonly used in reconstruction surgery. However, distal necrosis of the flap remains a difficult problem plastic Many studies have shown that activation autophagy is an important means maintaining cell homeostasis and can improve survival rate flaps. In current study, we investigated whether liraglutide promote random by stimulating autophagy. Our results show significantly viability, increase blood flow, reduce tissue oedema. addition, demonstrated stimulate...

Abstract Cirratiomycin, a heptapeptide with antibacterial activity, was isolated and characterized in 1981; however, its biosynthetic pathway has not been elucidated. It contains several interesting nonproteinogenic amino acids, such as (2 S ,3 )‐2,3‐diaminobutyric acid ((2 )‐DABA) α‐(hydroxymethyl)serine, building blocks. Here, we report the identification of cirratiomycin gene cluster Streptomyces cirratus . Bioinformatic analysis revealed that viridifaciens Kitasatospora aureofaciens...

Anterior cruciate ligament (ACL) tears are prevalent knee injures, particularly among active individuals. Accurate and timely diagnosis is essential for determining the optimal treatment strategy assessing patient prognosis. Various previous studies have demonstrated successful application of deep learning techniques in field medical image analysis. This study aimed to develop a model detecting ACL magnetic resonance Imaging (MRI) enhance diagnostic accuracy efficiency. The proposed consists...

In recent years, concerns have escalated regarding eye health problems arising from Light-emitting diode (LED), which emits high-energy blue light (BL), potentially causing corneal epithelial dysfunctions (CEpD). Nevertheless, the mechanisms underlying this damage remain poorly comprehended. This study endeavors to explore specific through BL exposure induces CEpD. The carried out diverse assays and treatments investigate toxicological effects of exposure. 48 hours (h) 440 nm decreased...

Background: Hyperthermia has proved successful in treating cutaneous human papillomavirus infectious diseases such as plantar wart and condyloma acuminata (CA). Moreover, this treatment provides improved therapeutic efficacy these conditions compared with conventional therapies.Objectives: To investigate the global proteome changes CA response to hyperthermia achieve a better understanding of mechanisms therapy against HPV-infectious diseases.Methods: tissue was obtained from patients...

The impact of chronic hepatic infection on antigen non-specific immune cells in circulation remains poorly understood. We reported lasting global hyperfunction peripheral CD8 T HCV-infected individuals with cirrhosis. Whether gene expression patterns bulk are associated the severity liver fibrosis HCV is not known.

Random-pattern skin flap replantation is generally used in the reconstruction of surgical tissues and covering a series defects. However, ischemia often occurs at distal parts, which lead to necrosis. Previous studies have shown that andrographolide (Andro) protects against ischemic cardiovascular diseases, but little known about effect Andro on viability. Thus, our study aimed building model random-pattern understand mechanism Andro-induced effects survival. In this study, fifty-four mice...

A random-pattern skin flap plays an important role in the field of wound repair; mechanisms that influence survival flaps have been extensively studied but little attention has paid to endogenous counterinjury substances and mechanism. Previous reports reveal apelin-APJ axis is mechanism considerable function protecting against infection, inflammation, oxidative stress, necrosis, apoptosis various organs. As vivo study, our study proved apelin/APJ protected by alleviating vascular stress...

Arterialized venous flap (AVF) is limited in clinical application because its survival remains inconsistent and exact mechanism still unclear. Hirudin an effective thrombin specific inhibitor, which isolated from the salivary gland secretions of leech. Our study evaluated impact hirudin on viability AVFs rabbits. Thirty-six rabbits were randomly divided into three groups: sham group (physiological perfusion), control (AVF), (AVF + hirudin). In group, 20 antithrombin units (ATU) (2.5 ml)...

Abstract The shape memory properties (SMP) of long‐chain branched poly(lactic acid) (b‐PLA) vascular stent prepared through 3D‐printed technologies are studied by comparison with PLA. SMP is expected to endow smart self‐expanding stent, which could self‐expand and support the stenosis after reaching lesion. recovery rate b‐PLA samples at 60°C reaches 93%, much higher than that PLA 26%. two‐cycle deformation‐recovery test dynamic mechanical analysis (DMA) shows 98% in first second cycle,...