A5040967178- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Gene Regulatory Network Analysis
- Developmental Biology and Gene Regulation
- Genetic Associations and Epidemiology
- Spinal Cord Injury Research
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Congenital heart defects research
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Muscle activation and electromyography studies
- Neuroscience of respiration and sleep
- Cerebral Palsy and Movement Disorders
- Signaling Pathways in Disease
- Birth, Development, and Health
- MicroRNA in disease regulation
- Medical Imaging and Pathology Studies
École Polytechnique Fédérale de Lausanne
2016-2023
Idorsia (Switzerland)
2020-2022
Karolinska Institutet
2005-2011
Gunma University
2005
Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and relationship to commonly used rodent models remain be defined. We performed single-cell RNA sequencing examine development in mouse. found 25 molecularly defined including five subtypes radial glia-like cells four progenitors. In mouse, two mature fetal dopaminergic neuron diversified into adult classes during postnatal development. Cell...
Highlights•Single-cell RNA sequencing of striatal interneurons reveals seven main classes•Subclass-specific latent factor analysis gradients gene expression•Pvalb neurons are not a discrete class but belong to expressing Pthlh•Interneuron classes vary in their similarity counterparts from cortex/hippocampusSummaryStriatal locally projecting neurons, or interneurons, act on nearby circuits and shape functional output the rest basal ganglia. We performed single-cell cells enriching for...
Motor neurons (MNs) are the principal in mammalian spinal cord whose activities cause muscles to contract. In addition their peripheral axons, MNs have central collaterals that contact inhibitory Renshaw cells and other MNs. Since its original discovery >60 years ago, it has been a general notion acetylcholine is only transmitter released from MN synapses both peripherally centrally. Here, we show, using multidisciplinary approach, MNs, acetylcholine, corelease glutamate excite but not...
In contrast to mammals, salamanders and teleost fishes can efficiently repair the adult brain. It has been hypothesised that constitutively active neurogenic niches are a prerequisite for extensive neuronal regeneration capacity. Here, we show highly regenerative salamander, red spotted newt, displays an unexpectedly similar distribution of germinal with mammals under normal physiological conditions. Proliferation zones in newt brain restricted forebrain, whereas all other regions...
In the progression phase of idiopathic pulmonary fibrosis (IPF), normal alveolar structure lung is lost and replaced by remodeled fibrotic tissue bronchiolized cystic airspaces. Although these are characteristic features IPF, knowledge specific interactions between pathological processes limited. Here, interaction epithelial mesenchymal cells was investigated in a coculture model human primary airway (EC) fibroblasts (FB). Single-cell RNA sequencing revealed that starting EC population...
The transcription factors Nkx2.2 and Nkx2.9 have been proposed to execute partially overlapping functions in neuronal patterning of the ventral spinal cord response graded sonic hedgehog signaling. present report shows that mice lacking both Nkx2 proteins, presumptive progenitor cells p3 domain neural tube convert motor neurons (MN) never acquire fate V3 interneurons. This result supports concept are required establish by repressing early MN lineage-specific program, including genes like...
Spinal cord injury leads to severe problems involving impaired motor, sensory, and autonomic functions. After spinal there is an initial phase of hyporeflexia followed by hyperreflexia, often referred as spasticity. Previous studies have suggested a relationship between the reappearance endogenous plateau potentials in motor neurons development spasticity after spinalization. To unravel molecular mechanisms underlying increased excitability return below we investigated changes gene...
Spinal cord injury leads to neurological dysfunctions affecting the motor, sensory as well autonomic systems. Increased excitability of motor neurons has been implicated in injury-induced spasticity, where reappearance self-sustained plateau potentials absence modulatory inputs from brain correlates with development spasticity. Here we examine dynamic transcriptional response spinal it evolves over time unravel common gene expression patterns and their underlying regulatory mechanisms. For...
The spike timing in rhythmically active interneurons the mammalian spinal locomotor network varies from cycle to cycle. We tested contribution passive membrane properties this variable firing pattern, by measuring reliability of timing, P, isolated neonatal rat cord, using intracellular injection sinusoidal command currents different frequencies (0.325-31.25 Hz). P is a measure precision timing. In general, was low at and amplitudes (P = 0-0.6; 0-1.875 Hz; 0-30 pA), high 0.8-1; 3.125-31.25...
Objectives: SLE is an autoimmune disease characterised by aberrant lymphocyte activation and autoantibody production. This study provides in-depth preclinical clinical characterisation of the treatment effect cenerimod, a sphingosine-1-phosphate receptor type 1 (S1P ) modulator, in SLE. Methods: Cenerimod on numbers, organ pathology, inflammation, survival was evaluated MRL/lpr lupus mouse model. Lymphocytes from healthy subjects patients with were assessed for cenerimod-induced S1P...
Abstract With few exceptions, the marked advances in knowledge about genetic basis for schizophrenia have not converged on findings that can be confidently used precise experimental modeling. Applying of cellular taxonomy brain from single-cell RNA-sequencing, we evaluated whether genomic loci implicated map onto specific cell types. The common variant results consistently mapped to pyramidal cells, medium spiny neurons, and certain interneurons but far less embryonic, progenitor, or glial...
Summary Recent work suggests that cortical interneuron diversity arises from genetic mechanisms guided by the interplay of intrinsic developmental patterning and local extrinsic cues. Individual programs underlying subtype identity are at least partly established in postmitotic neural precursors, prior to their tangential migration integration circuitry. Nevertheless, it is unclear how distinct identities maintained during maturation. Sox6 a transcription factor with an role MGE-derived...
In the field of neuroscience microarray gene expression profiles on anatomically defined brain structures are being used increasingly to study both normal functions as well pathological states. Fluorescent tracing techniques in tissue that identifies distinct neuronal populations can combination with global profiling potentially increase resolution and specificity such studies shed new light at cellular level.We examine two spinal cord neonatal rat, principal motor neurons specific...
There was an error in the ePress version of Development137, 4127-4134 published on 10 November 2010.On p. 4133, title Fig. 6 incorrect. The correct appears below:Adult midbrain DA regeneration depends hedgehog signalling.The online issue and print copy are correct.We apologise to authors readers for this mistake.