A5052795184- Plant tissue culture and regeneration
- Growth and nutrition in plants
- Developmental Biology and Gene Regulation
- Seed Germination and Physiology
- Neurogenesis and neuroplasticity mechanisms
- Plant Physiology and Cultivation Studies
- Pluripotent Stem Cells Research
- Flowering Plant Growth and Cultivation
- Congenital heart defects research
- Muscle Physiology and Disorders
- Zebrafish Biomedical Research Applications
- Hedgehog Signaling Pathway Studies
- Plant and animal studies
- Seedling growth and survival studies
- Postharvest Quality and Shelf Life Management
- Genetic and phenotypic traits in livestock
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Fern and Epiphyte Biology
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Biochemical and biochemical processes
- Plant Pathogens and Fungal Diseases
- Congenital limb and hand anomalies
- Nuclear Receptors and Signaling
Karolinska Institutet
2014-2024
Universidade Federal de Viçosa
2005-2022
New York State College of Veterinary Medicine
2016
Cornell University
2016
Rockefeller University
2008
University of Florida
2008
Brazilian Agricultural Research Corporation
2003
Cells adapt to hypoxia by a cellular response, where hypoxia-inducible factor 1alpha (HIF-1alpha) becomes stabilized and directly activates transcription of downstream genes. In addition this "canonical" certain aspects the pathway require integration with Notch signaling, i.e., HIF-1alpha can interact intracellular domain (ICD) augment response. work, we demonstrate an additional level complexity in cross-talk: factor-inhibiting HIF-1 (FIH-1) regulates not only HIF activity, but also...
Neural progenitor cells often produce distinct types of neurons in a specific order, but the determinants that control sequential generation neuronal subclasses vertebrate CNS remain poorly defined. We examined visceral motor and serotonergic from common pool neural progenitors located ventral hindbrain. found temporal specification these varies along anterior-posterior axis hindbrain, timing their critically depends on integrated activities Nkx- Hox-class homeodomain proteins. A primary...
Tbx20, a member of the T-box family transcriptional regulators, shows evolutionary conserved expression in developing heart. In mouse, Tbx20 is expressed cardiac crescent, then endocardium and myocardium linear looped heart tube before it restricted to atrioventricular canal outflow tract multi-chambered Here, we show that required for progression from Mice carrying targeted mutation early embryonic lethality due hemodynamic failure. A with normal anteroposterior patterning established...
Abstract Insulin binds the insulin receptor (IR) and regulates anabolic processes in target tissues. Impaired IR signalling is associated with multiple diseases, including diabetes, cancer neurodegenerative disorders. IRs have been reported to form nanoclusters at cell membrane several types, even absence of binding. Here we exploit nanoscale spatial organization achieve controlled multivalent activation. To control valency, developed rod-like insulin–DNA origami nanostructures carrying...
The genetic program that underlies the generation of visceral motoneurons in developing hindbrain remains poorly defined. We have examined role Nkx6 and Nkx2 class homeodomain proteins this process, provide evidence these mediate complementary roles specification motoneuron fate. expression Nkx2.2 progenitor cells is sufficient to activation Phox2b, a protein required for motoneurons. redundant activities Nkx6.1 Nkx6.2, turn, are dispensable but necessary prevent from adopting parallel...
The deployment of morphogen gradients is a core strategy to establish cell diversity in developing tissues, but little known about how small differences the concentration extracellular signals are translated into robust patterning output responding cells. We have examined activity homeodomain proteins, which presumed operate downstream graded Shh signaling neural patterning, and describe feedback circuit between pathway transcription factors that establishes non-graded regulation activity....
Skeletal muscle plasticity and remodeling are critical for adapting tissue function to use, disuse, regeneration. The aim of this study was identify genes molecular pathways that regulate the transition from atrophy compensatory hypertrophy or recovery injury. Here, we have used a mouse model hindlimb unloading reloading, which causes skeletal atrophy, regeneration hypertrophy, respectively. We analyzed at reloading changes in transcriptome extracellular fluid proteome. then qRT-PCR,...
Intestinal hormone-producing cells represent the largest endocrine system in body, but remarkably little is known about enteroendocrine cell type specification embryo and adult. We analyzed stage- type-specific deletions of Nkx2.2 its functional domains order to characterize role development maintenance lineages mouse duodenum colon. Although regulates at all stages examined, it controls differentiation progressively fewer populations when deleted from Ngn3(+) progenitor or adult duodenum....
Abstract Stem cell therapies for Parkinson’s disease (PD) have entered first-in-human clinical trials using a set of technically related methods to produce mesencephalic dopamine (mDA) neurons from human pluripotent stem cells (hPSCs). Here, we outline an approach high-yield derivation mDA that principally differs alternative technologies by utilizing retinoic acid (RA) signaling, instead WNT and FGF8 specify fate. Unlike most morphogen signals, where precise concentration determines fate,...
The transcription factors Nkx2.2 and Nkx2.9 have been proposed to execute partially overlapping functions in neuronal patterning of the ventral spinal cord response graded sonic hedgehog signaling. present report shows that mice lacking both Nkx2 proteins, presumptive progenitor cells p3 domain neural tube convert motor neurons (MN) never acquire fate V3 interneurons. This result supports concept are required establish by repressing early MN lineage-specific program, including genes like...
Skeletal muscle cells contain hundreds of myonuclei within a shared cytoplasm, presenting unique challenges for regulating gene expression. Certain transcriptional programs (e.g., postsynaptic machinery) are segregated to specialized domains, while others contractile proteins) do not show spatial confinement. Furthermore, local stimuli, such as denervation, can induce responses that propagated along the cells. Regulated transport nuclear proteins transcription factors) between represents...
Pollination effectiveness was evaluated for pollen (pollinia) from two Dendrobium hybrids, ‘Sena Red’ and ‘Mini WRL’, submitted to cryopreservation using a vitrification protocol. Parameters included pollinia exposure previtrification solution (PVS2) under ice (0 °C) or room (27 ± 2 temperatures 1 4 hours before (LN). On removal cryopreservation, were used pollinate flowers of the same hybrids verify viability germination. All showed high percentages germination (greater than 80%) after...
Skeletal muscle atrophy is a morbidity and mortality risk factor that happens with disuse, chronic disease, aging. The tissue remodeling during recovery from or injury involves changes in different cell types such as fibers, satellite immune cells. Here, we show the previously uncharacterized gene protein Zfp697 damage-induced regulator of remodeling. Zfp697/ZNF697 expression transiently elevated mice humans. Sustained mouse leads to signature chemokine secretion, recruitment, extracellular...
Bone morphogenetic proteins (BMPs) secreted by the dorsal neural tube and overlying ectoderm are key signals for specification of roof plate interneuron populations. However, that confer nonneurogenic character to region largely unknown. We report shows elevated oxygen levels compared neurogenic regions tube. These high required expression antineuronal transcription factor Hes1 in region. The transcriptional corepressor CtBP is a critical mediator oxygen-sensing response. High promotes...
The new web resource EviNet provides an easily run interface to network enrichment analysis for exploration of novel, experimentally defined gene sets. major advantages this are (i) applicability any genes found in the global rather than only those with pathway/ontology term annotations, (ii) ability connect via different molecular mechanisms within one high-throughput platform, and (iii) statistical power sufficient detect very small sets, down individual genes. users' sets either prior...
Este trabalho teve como objetivo avaliar a eficiência do enraizamento adventício em estacas extraídas de brotações obtidas por meio da decepa árvore, anelamento caule e indução epicórmicas galhos podados árvores Eucalyptus cloeziana, bem influência AIB no dessas estacas. Foram utilizadas cepas 3 5 15 anos idade, respectivamente, para o resgate cepas. Para caule, foram 5, 20 enquanto as eram idade. A mostrou melhores resultados selecionadas com relação àquelas anos. No caso das advindas...
Este trabalho teve como objetivo avaliar a eficiência das auxinas AIB (ácido indolbutírico) e ANA naftalenoacético) no enraizamento adventício de miniestacas clones Eucalyptus cloeziana. Foram utilizadas provenientes sete cloeziana, estabelecidos em minijardim clonal, sendo avaliados os efeitos (0, 1.500, 3.000 6.000 mg L-1) na forma líquida pó líquida. Os resultados apontaram ser miniestaquia técnica viável propagação vegetativa dos cloeziana estudados, apresentando, modo geral, alto índice...
Cranial motor nerves in vertebrates are comprised of the three principal subtypes branchial, visceral, and somatic neurons, which develop typical patterns along anteroposterior dorsoventral axes hindbrain. Here we demonstrate that formation branchial visceral neurons critically depends on transcription factors Nkx2.2 Nkx2.9, together determine cell fate neuronal progenitor cells. Disruption both genes mouse embryos results complete loss vagal spinal accessory nerves, partial facial...
Orchid fertilization is fundamental for a satisfactory plant growth and development commercial orchid production as well in collections. Mineral and/or organic sources can be used fertilization. The objective of this study was to evaluate the effect use mineral fertilizers on nutrition (Laelia purpurata 'werkhanserii' x L. lobata 'Jeni') seedlings greenhouse. following were tested: an NPK fertilizer + micronutrients; Ca source form calcium nitrate; two fertilizers, one prepared with mixture...
Este estudo teve como objetivo avaliar a eficiência dos antioxidantes ácido ascórbico, carvão ativado e polivinilpirrolidona (PVP) no enraizamento de miniestacas quatro clones Eucalyptus grandis x E. urophylla. As foram coletadas em minijardim clonal desenvolvido sistema hidroponia canaletas. Experimentalmente, testadas cinco concentrações sete PVP, nos estudados. Foram realizadas avaliações quanto ao porcentual crescimento das enraizadas casa vegetação, sombra pleno sol. Concluiu-se que as...
There is a great demand for simpler and less costly laboratory techniques more accessible procedures orchid breeders who do not have the necessary theoretical basis to use traditional seed clone production methods of orchids in vitro. The aim this study was assess sodium hypochlorite (NaClO) as decontaminant process inoculating adult explants Arundina bambusifolia Epidendrum ibaguenses. Solutions NaClO (1.200, 2.400, 3.600, 4.800 6.000 mg L-1 - equivalent 50, 100, 150, 200 250 mL commercial...