Various fungi have the ability to colonize surfaces and form biofilms. Fungal biofilm-associated infections are frequently refractory targeted treatment because of resistance antifungal drugs. One fungus that colonises respiratory tract cystic fibrosis (CF) patients is opportunistic black yeast-like Exophiala dermatitidis. We investigated biofilm-forming E. dermatitidis its susceptibility various antiinfective agents natural compounds. tested 58 isolates with a biofilm assay based on crystal...

Stenotrophomonas maltophilia is a non-fermenting Gram-negative bacterium that ubiquitous in the environment. In humans, this opportunistic multi-drug resistant pathogen responsible for plethora of healthcare-associated infections. Here, we utilized whole genome sequencing (WGS) based phylogenomic core single nucleotid polymorphism (SNP) approach to characterize S. subgroups, their potential association with human infection, and detect any possible transmission events. total, 89 isolates (67...

Abstract Objectives Patients with immunodeficiency or cystic fibrosis frequently suffer from respiratory fungal infections. In particular, biofilm-associated fungi cause refractory infection manifestations, linked to increased resistance anti-infective agents. One emerging filamentous fungus is Lomentospora prolificans. Here, the biofilm-formation capabilities of L. prolificans isolates were investigated and susceptibility biofilms various antifungal agents was analysed. Methods Biofilm...

This is the first study comparing three commercially available PCR assays for detection of Aspergillus DNA from respiratory specimen immunocompromised patients and presence cyp51A gene mutations. Bronchoalveolar lavages (BALs, N = 103) with haematological/oncological underlying diseases were retrospectively investigated. The performance assays, namely MycoGENIE®Aspergillus fumigatus Real-Time Kit (Adamtech), Fungiplex®Aspergillus Azole-R IVD (Bruker Daltonik GmbH) AsperGenius® (PathoNostics...

Interest in aspergillosis has increased over the past decades. An increase incidence of azole-resistant Aspergillus fumigatus strains been reported; therefore, need for novel therapeutic approaches is urgent. The formation biofilms contributes to pathogen resistance. We investigated biofilm capabilities A. and analysed susceptibility at various developmental stages three antifungal agents.Biofilm 19 clinical (3 azole-susceptible 16 strains) was determined by crystal violet staining an XTT...

The capability of Pseudomonas aeruginosa and Staphylococcus aureus to form biofilm on varying CI component materials differs in the presence absence bioactive glass (BAG). application BAG induces significant changes morphology which can be visualized via scanning electron microscopy (SEM). Bacterial formation medical devices, such as cochlear implants (CI), lead chronic infections. Interestingly, type S53P4 seems a promising tool for use reduction development. Primarily, four bacterial...

Summary Background The number of invasive Candida infections has significantly increased in recent decades. For the successful treatment fungal infections, rapid identification at species level, particularly polyfungal is a key factor. In this study, four commercially available chromogenic media, Candi Select ™ 4 (CS4), chromID™ Agar (CCA), BBL™ CHROMagar™ Medium (BBL) and Brilliance™ (BCA) were evaluated for identification. Material/Methods Overall, 181 bronchial secretion samples from...

Abstract Numbers of infections with azole-resistant Aspergillus fumigatus (ARAf) were rising in the last decades. We assessed ARAf susceptibility trends towards five antifungal agents (amphotericin B (AMB), itraconazole (ITR), voriconazole (VCZ), olorofim (OLO) and manogepix (MGX)) over twelve years a German Excellence Center for Medical Mycology (ECMM). In addition, underlying mutations studied correlated minimum inhibitory concentration (MIC). Broth microdilution (BMD) was performed...

A commercially available loop-mediated isothermal amplification assay (LAMP) for the detection of Pneumocystis jirovecii (P. jirovecii) has been evaluated diagnosis pneumonia (PcP) in critically ill patients. Altogether, 109 lower respiratory tract specimens from 95 patients with a positive P. test routine diagnostics were collected five distinct university hospitals Germany. All samples tested qPCR and eazyplex® LAMP assay. was set as gold standard beforehand 100 categorized to have proven,...

COVID-19-associated invasive pulmonary aspergillosis (CAPA) is associated with increased mortality. Cases of CAPA caused by azole-resistant Aspergillus fumigatus strains have been reported.To analyse the twelve-month prevalence in a German tertiary care hospital and to characterise clinical A. isolates from two hospitals antifungal susceptibility testing microsatellite genotyping.Retrospective observational study critically ill adults intensive units COVID-19 17 February 2020 until 16 2021...

Biofilm formation on cochlear implant (CI) surfaces differs between bacterial species and can be reduced by the application of S53P4 bioactive glass.The biofilms medical devices, such as implants, lead to chronic infections resulting in need for removal. In this study, various three CI kits from different manufacturers were examined biofilm reduction a pre-existing glass.Biofilm formations 4 causing implant-related tested 17 surfaces: Pseudomonas aeruginosa (ATCC9027), Staphylococcus aureus...

Research into the cooperative pathogenicity of microbes in cystic fibrosis (CF) lungs is crucial for an understanding pathophysiology infections and development novel treatment strategies. This study investigated impact common CF-associated bacterial pathogen Pseudomonas aeruginosa on black yeast Exophiala dermatitidis. It evaluated planktonic growth, biofilm formation, morphology, virulence fungus presence or absence P. aeruginosa. also determined role quorum-sensing (QS) molecules within...

Summary Background Various tools are obtainable for the detection of Pneumocystis jirovecii , among them qPCR promising highest sensitivity. A novel molecular method is commercially available, loop‐mediated isothermal amplification (LAMP) assay. Objectives We compared performance LAMP eazyplex ® with RealStar PCR 1.0 qPCR. Material/Methods Overall, 162 lower respiratory tract specimens from 146 critically ill patients were investigated. assay and carried out according to manufacturer's...

Summary Background Commonly, the application of radiological and clinical criteria determination galactomannan (GM) in respiratory samples are used as a diagnostic tool for detection invasive pulmonary aspergillosis (IPA). Materials/methods In this study, two lateral flow assays, OLM Aspergillus device (LFD) IMMY sōna Galactomannan assay (LFA), were evaluated at tertiary hospitals Germany. A total 200 bronchoalveolar lavage (BAL) from patients with suspicion IPA analysed retrospectively. LFD...

This study investigated whether sphingosine is effective as prophylaxis against Aspergillus spp. and Candida In vitro experiments showed that very efficacious A. fumigatus Nakeomyces glabrataa (formerly named C. glabrata). A mouse model of invasive aspergillosis exerts a prophylactic effect sphingosine-treated animals exhibit strong survival advantage after infection. Furthermore, mechanistic studies treatment with leads to the early depolarization mitochondrial membrane potential (Δψm)...

Infections with Scedosporium spp. and Lomentospora prolificans have become a serious threat in clinical settings. The high mortality rates associated these infections can be correlated their multidrug resistance. development of alternative treatment strategies has crucial. Here, we investigate the vitro vivo activity luliconazole (LLCZ) against apiospermum (including its teleomorph Pseudallescheria boydii) prolificans. LLCZ MICs were determined for total 37 isolates (31 L. isolates, 6...

Abstract Exophiala dermatitidis causes chromoblastomycosis, phaeohyphomycosis and fatal infections of the central nervous system patients with Asian background. It is also found in respiratory secretions from cystic fibrosis (CF) patients. In this study a variety E. strains (isolates Asia, environmental CF) were characterized their pathogenicity by survival analyzes using two different invertebrate host organisms, Caenorhabditis elegans Galleria mellonella . Furthermore, morphological...

Abstract The opportunistic black yeast-like fungus Exophiala dermatitidis frequently colonizes the respiratory tract of cystic fibroses (CF) patients. Additionally, it can cause superficial, systemic, and cerebral forms phaeohyphomycoses. objective this study was to develop apply a microsatellite or short tandem repeat (STR) genotyping scheme for E. . In total, 82 isolates from various geographic origins (environmental = 9, CF 63, invasive melanin-deficient mutant 1) were included in study....

Candida auris has become a global fungal public health threat. This multidrug-resistant yeast is associated with nosocomial intra- and interhospital transmissions causing healthcare-associated infections. Here, we report on two C. cases from Germany. The patients stayed in Germany for long time before was detected during their hospitalization. were isolated single rooms contact precautions. No within the hospital. Both isolates exhibited high minimum inhibitory concentrations (MICs) of...