A5062444640- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Metabolomics and Mass Spectrometry Studies
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- SARS-CoV-2 and COVID-19 Research
- Bioinformatics and Genomic Networks
- Tryptophan and brain disorders
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- Advanced Chemical Sensor Technologies
- Viral Infections and Outbreaks Research
- Protein Structure and Dynamics
- Neurological disorders and treatments
- Heat shock proteins research
- Adipose Tissue and Metabolism
- Ginger and Zingiberaceae research
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- PARP inhibition in cancer therapy
- Mangiferin and Mango Extracts
Hebei Medical University
2025
University of Chinese Academy of Sciences
2018-2024
Stanford University
2022-2024
Howard Hughes Medical Institute
2022-2024
Shanghai Institute of Organic Chemistry
2016-2024
Chinese Academy of Sciences
2016-2024
Chengdu University of Traditional Chinese Medicine
2024
Shaoxing University
2022
Aging is a complex process associated with nearly all diseases. Understanding the molecular changes underlying aging and identifying therapeutic targets for aging-related diseases are crucial increasing healthspan. Although many studies have explored linear during aging, prevalence of mortality risk accelerates after specific time points, indicating importance studying nonlinear changes. In this study, we performed comprehensive multi-omics profiling on longitudinal human cohort 108...
Abstract Current healthcare practices are reactive and use limited physiological clinical information, often collected months or years apart. Moreover, the discovery profiling of blood biomarkers in research settings constrained by geographical barriers, cost inconvenience in-clinic venepuncture, low sampling frequency depth molecular measurements. Here we describe a strategy for frequent capture analysis thousands metabolites, lipids, cytokines proteins 10 μl alongside information from...
The spread of pathological α-synuclein (α-syn) is a crucial event in the progression Parkinson's disease (PD). Cell surface receptors such as lymphocyte activation gene 3 (LAG3) and amyloid precursor-like protein 1 (APLP1) can preferentially bind α-syn over monomeric state to initiate cell-to-cell transmission. However, molecular mechanism underlying this selective binding unknown. Here, we perform an array biophysical experiments reveal that LAG3 D1 APLP1 E1 domains commonly use alkaline...
Reproducibility, traceability, and transparency have been long-standing issues for metabolomics data analysis. Multiple tools developed, but limitations still exist. Here, we present the tidyMass project ( https://www.tidymass.org/ ), a comprehensive R-based computational framework that can achieve traceable, shareable, reproducible workflow needs of processing analysis LC-MS-based untargeted metabolomics. TidyMass is an ecosystem R packages share underlying design philosophy, grammar,...
Significance Emergence of viral pathogens necessitates new approaches to study fusion and entry into host cells. A key step in mediating involves the formation a six-helix bundle within spike protein. Rapid structural characterization this state has been difficult, hindering understanding emerging variants. We developed method efficiently determine high-resolution structures by molecular scaffolding cryogenic electron microscopy. Using method, we determined severe acute respiratory syndrome...
Tracking and imaging immune cells in vivo non-invasively would offer insights into the responses induced by vaccination. Here we report a cancer vaccine consisting of polymer-coated NaErF
Synaptic vesicles are organelles with a precisely defined protein and lipid composition
ABSTRACT Accumulation of aggregated α-synuclein (α-syn) in Lewy bodies is the pathological hallmark Parkinson's disease (PD). Genetic mutations lipid metabolism are causative for a subset patients with Parkinsonism. The role α-syn's interactions its function and aggregation recognized, yet specific lipids involved how issues trigger α-syn neurodegeneration remain unclear. Here, we found that shows preference binding to lysophospholipids (LPLs), particularly targeting lysophosphatidylcholine...
Many human neurodegenerative diseases are associated with amyloid fibril formation. Inhibition of formation is importance for therapeutics the related diseases. However, development selective and potent inhibitors remains challenging. Here based on structures A fibrils their amyloid-forming segments, we designed a series peptide using RosettaDesign. We further utilized chemical scaffold to constrain peptides into β-strand conformation, which significantly improves potency against aggregation...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge currently available disease 2019 vaccines and monoclonal antibody therapies through epitope change on the receptor binding domain viral spike glycoprotein. Hence, there is a specific urgent need for alternative antivirals that target processes less likely to be affected by mutation, such as membrane fusion step entry into host cell. One antiviral class includes peptide inhibitors, which block formation...
Synaptic vesicle docking and priming are dynamic processes. At the molecular level, SNAREs (soluble NSF attachment protein receptors), synaptotagmins, other factors critical for Ca
The incidence of inflammatory bowel disease (IBD) and the associated risk colon cancer are increasing globally. Traditional Chinese medicine (TCM) treatment has unique advantages. Sishen Pill, a common patented drug used to treat abdominal pain diarrhea, consists mainly Psoraleae Fructus, Myristicae Semen, Euodiae Schisandra Chinensis. Modern research confirmed that Pill its active secondary metabolites, such as psoralen, myristicin, evodiamine, schisandrin, can improve intestinal...
Vesicle associated membrane protein 2 (VAMP2/synaptobrevin2), a core SNARE residing on synaptic vesicles (SVs), forms helix bundles with syntaxin-1 and SNAP25 for the assembly. Prior to assembly, structure of VAMP2 is unclear. Here, by using in-cell NMR spectroscopy, we describe dynamic association motif in mammalian cells, structural change upon intracellular lipid environment. We analyze compositions SV mass-spectrometry-based lipidomic profiling, further reveal that distinctive...
α-synuclein (α-Syn) is a presynaptic protein that involved in Parkinson’s and other neurodegenerative diseases binds to negatively charged phospholipids. Previously, we reported α-Syn clusters synthetic proteoliposomes mimic synaptic vesicles. This vesicle-clustering activity depends on specific interaction of with anionic Here, report surprisingly also interacts the neutral phospholipid lysophosphatidylcholine (lysoPC). Even absence lipids, lysoPC facilitates α-Syn-induced vesicle...
The intrinsically disordered protein, Tau, is abundant in neurons and contributes to the regulation of microtubule (MT) actin network, while its intracellular abnormal aggregation closely associated with Alzheimer’s disease. Here, using in-cell Nuclear Magnetic Resonance (NMR) spectroscopy, we investigated conformations two different isoforms Tau40 k19, mammalian cells. Combined immunofluorescence imaging western blot analyses, found that isotope-enriched which was delivered into cultured...
Tau hyper-phosphorylation and deposition into neurofibrillary tangles have been found in brains of patients with Alzheimer’s disease (AD) other tauopathies. Molecular chaperones are involved regulating the pathological aggregation phosphorylated (pTau) modulating progression. Here, we report that nicotinamide mononucleotide adenylyltransferase (NMNAT), a well-known NAD+ synthase, serves as chaperone pTau to prevent its amyloid vitro well mitigate pathology fly tauopathy model. By combining...