A5005477538- Parasitic Infections and Diagnostics
- Parasitic infections in humans and animals
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Coccidia and coccidiosis research
- Epigenetics and DNA Methylation
- Insect symbiosis and bacterial influences
- Parasite Biology and Host Interactions
- Congenital Anomalies and Fetal Surgery
- Vector-borne infectious diseases
University of Bern
2015-2021
The metacestode stage of the fox tapeworm Echinococcus multilocularis causes lethal disease alveolar echinococcosis. Current chemotherapeutic treatment options are based on benzimidazoles (albendazole and mebendazole), which insufficient hence alternative drugs needed. In this study, we screened 400 compounds Medicines for Malaria Venture (MMV) Pathogen Box against E. metacestodes. For screen, employed phosphoglucose isomerase (PGI) assay assesses drug-induced damage metacestodes, identified...
The lethal disease alveolar echinococcosis (AE) is caused by the metacestode stage of fox tapeworm Echinococcus multilocularis. Current chemotherapeutical treatment AE relies on albendazole and mebendazole, with caveat that these compounds are not parasiticidal. Drugs have to be taken for a prolonged period time, often life-long, which can cause adverse effects reduces patients' quality life. In some individuals, benzimidazoles inactive or toxicity, leading discontinuation. Alternatives...
The naphthoquinone buparvaquone is currently the only drug used against theileriosis. Here, effects of were investigated in vitro and an experimental mouse model for Neospora caninum infection. In 4-day proliferation assays, efficiently inhibited N. tachyzoite replication (IC50 = 4.9 nM; IC100 100 nM). However, long term tachyzoites adapted resumed presence nM after 20 days cultivation. Parasiticidal activity was noted 9 culture 0.5 µM or 6 1 buparvaquone. TEM infected fibroblasts treated...
The cestode E. multilocularis causes the disease alveolar echinococcosis (AE) in humans. continuously proliferating metacestode (larval stage) of parasite infects mostly liver and exhibits tumor-like growth. Current chemotherapeutical treatment options rely on benzimidazoles, which are rarely curative have to be applied daily life-long. This can result considerable hepatotoxicity thus discontinuation. Therefore, novel drugs against AE urgently needed. anti-malarial mefloquine was previously...
Alveolar echinococcosis (AE) is a zoonotic disease that deadly if left untreated. AE caused by the larval metacestode stage of cestode Echinococcus multilocularis. Better knowledge on host-parasite interface could yield novel targets for improvement treatment against AE. We analyzed culture media incubated with in vitro grown E. multilocularis metacestodes 1H nuclear magnetic resonance spectroscopy to identify unknown metabolic footprint parasite. Moreover, we quantitatively all amino acids,...
Intestinal cestodes are infecting millions of people and livestock worldwide, but treatment is mainly based on one drug: praziquantel. The identification new anti-cestodal compounds hampered by the lack suitable screening assays. It difficult, or even impossible, to evaluate drugs against adult in vitro due fact that these parasites cannot be cultured microwell plates, larval stages most cases represent different organisms terms size, morphology, metabolic requirements. We here present an...
the apicomplexan parasite Neospora caninum causes important reproductive problems in farm animals, most notably cattle. After infection via oocysts or tissue cysts, rapidly dividing tachyzoites infect various tissues and organs, immunocompetent hosts, they differentiate into slowly bradyzoites, which form cysts constitute a resting stage persisting within infected tissues. Bumped kinase inhibitors (BKIs) of calcium dependent protein 1 are promising drug candidates for treatment infections....
Theileria annulata is an apicomplexan parasite that modifies the phenotype of its host cell completely, inducing uncontrolled proliferation, resistance to apoptosis, and increased invasiveness. The infected thus resembles a cancer cell, changes various signalling pathways accompany transformation. Most molecular mechanisms leading Theileria-induced immortalization leukocytes remain unknown. dissolves surrounding membrane soon after invasion starts interacting with proteins, ensuring...
Summary C. elegans has recently emerged as a valuable model to understand the link between nuclear organization and cell fate, by combining microscopy approaches, genome‐wide mapping techniques with advanced genetics. Crucial these analyses are determine interaction pattern of proteins DNA. Chromatin immunoprecipitation proven but it requires considerable amounts starting material. This is sometimes difficult achieve, in particular for specific genotypes (balanced strains, different sexes,...
Exposure of Neospora caninum tachyzoites to BKI-1294 in vitro results the formation long-lived multinucleated complexes (MNCs). However, vivo treatment BALB/c mice with shortly after N. infection during pregnancy was safe and profoundly reduced pup mortality vertical transmission. We hypothesized that MNCs could trigger immune responses contribute BKI efficacy vivo. In this study, were first vaccinated a sublethal dose treated BKI-1294. then investigated effects these treatments mating...