Histones are abundant nuclear proteins that essential for the packaging of eukaryotic DNA into chromosomes. Different histone variants, in combination with their modification 'code', control regulation gene expression diverse cellular processes. Several enzymes catalyze addition and removal multiple modifications have been discovered past decade, enabling investigations role(s) normal processes pathological conditions. This sudden influx data, however, has resulted need an updated...

The failure of the adult injured spinal cord to support axonal regeneration is in part attributed glial scar. Reactive astrocytes constitute a major cellular component scar and are heterogeneous with respect extracellular matrix proteins that they secrete. Astrocytes may produce antiregenerative molecules such as chondroitin sulphate proteoglycans (CSPGs) or proregenerative laminin fibronectin. While many different CSPGs expressed after injury (SCI) all rely on same enzymes,...

The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model chromosome-wide regulation. In Caenorhabditis elegans , DC is achieved by twofold down-regulation from both Xs in hermaphrodites. We show that males, single interacts with nuclear pore proteins, while hermaphrodites, complex (DCC) impairs this interaction and alters localization. Our results put forward structural which X-specific sequences locate...

The LINC complex tethers the cell nucleus to cytoskeleton regulate mechanical forces during migration, differentiation, and various diseases. function of complexes relies on interaction between highly conserved SUN KASH proteins that form higher-order assemblies capable load bearing. These structural details have emerged from in vitro assembled complexes; however, principles vivo assembly remain obscure. Here, we report a conformation-specific SUN2 antibody as tool visualize dynamics situ....

In lysozyme amyloidosis, fibrillar aggregates of are associated with severe renal, hepatic, and gastrointestinal manifestations, no definite therapy. Current drugs now being tested in amyloidosis clinical trials as aggregation inhibitors to mitigate disease progression. The tetracycline group among antimicrobials use is phase II trials, whereas some macrolides cephalosporins have shown neuroprotection. the present study, two cephalosporins, ceftazidime (CZD) cefotaxime (CXM), a glycopeptide,...

The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations probing genome-wide protein-DNA interactions specific the periphery. Here, we developed a new sensitive method, NPC-DamID, which combines vitro reconstitution import and DamID technology. fixation-free method identifies chromatin at NPCs intact nuclei from cells tissues. We found that are preferentially associated with common hierarchically arranged super-enhancers (SEs)...

Summary C. elegans has recently emerged as a valuable model to understand the link between nuclear organization and cell fate, by combining microscopy approaches, genome‐wide mapping techniques with advanced genetics. Crucial these analyses are determine interaction pattern of proteins DNA. Chromatin immunoprecipitation proven but it requires considerable amounts starting material. This is sometimes difficult achieve, in particular for specific genotypes (balanced strains, different sexes,...

The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations probing genome-wide protein-DNA interactions specific the periphery. Here, we developed a new sensitive method, NPC-DamID, which combines vitro reconstitution import and DamID technology. fixation-free method identifies chromatin at NPCs intact nuclei from cells tissues. We found that are preferentially associated with common hierarchically arranged super-enhancers (SEs)...

Animal sex is determined by the number of X chromosomes in many species, creating unequal gene dosage (aneuploidy) between sexes. Dosage Compensation mechanisms equalize this difference regulating X-linked expression. In nematode C. elegans current model suggests that DC achieved a 2-fold transcriptional downregulation hermaphrodites mediated Complex (DCC), which restricts access to RNA Polymerase II an unknown mechanism. Taking nuclear organization point view, we showed male chromosome...

DNA-FISH remains the method of choice to visualize genomic regions in situ ranging from a single locus entire chromosomes. Current methods generate probes rely on expensive kits that vary labeling efficiency and are limited by size and/or amount starting material fluorophores. Here we describe protocol prepare inexpensive ($20) using an isothermal polymerase, incorporating labeled nucleotides while amplifying minute amounts any template (PCR fragments/BAC/YAC/fosmids). For complete details...

ABSTRACT The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations probing genome-wide protein-DNA interactions specific the periphery. Here, we developed a new sensitive method, NPC-DamID, which combines vitro reconstitution import and DamID technology. fixation-free method identifies chromatin at NPCs intact nuclei from cells tissues. We found that are preferentially associated with common hierarchically arranged...

The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins play vital role in system's defense mechanism. Among five classes immunoglobulins, Immunoglobin G (IgG) most abundant widely studied. This article provides an in-depth overview basics IgG, including their chemical physical properties roles system. then focuses on critical biosensor working...

<div>Abstract<p>Altered expression of the Fas ligand (FasL)/Fas ratio exhibits a direct impact on prognosis cancer patients, and its impairment in cells may lead to apoptosis resistance. Thus, development effective therapies targeting FasL/Fas system play an important role fight against cancer. In this study, we evaluated whether fusion protein (hcc49scFv-FasL) comprising cytotoxicity domain FasL fused humanized antibody (CC49) tumor-associated glycoprotein 72, which is expressed...

Abstract The LINC complex tethers the cell nucleus to cytoskeleton regulate mechanical forces during migration, differentiation, and diseases. function of complexes relies on interaction between highly conserved SUN KASH proteins that form higher-order assemblies capable load bearing. These structural details have emerged from in vitro assembled however, principles vivo assembly remain obscure. Here, we report a conformation-specific SUN2 antibody as tool visualize dynamics situ . Using...