A5036380407- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Folate and B Vitamins Research
- Blood groups and transfusion
- Bone and Joint Diseases
- Epigenetics and DNA Methylation
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Health and Biochemistry
- Reproductive Health and Contraception
- Inflammasome and immune disorders
- Kruppel-like factors research
- Maternal and Neonatal Healthcare
- Pneumonia and Respiratory Infections
- Paraoxonase enzyme and polymorphisms
- Regulation of Appetite and Obesity
- MicroRNA in disease regulation
- Pesticide Exposure and Toxicity
- Metabolism and Genetic Disorders
- Genital Health and Disease
- Liver Disease Diagnosis and Treatment
- Streptococcal Infections and Treatments
- Mitochondrial Function and Pathology
Fundação Oswaldo Cruz
2015-2023
Universidade Federal da Bahia
2014-2022
Sickle cell disease (SCD) consists of a group hemoglobinopathies in which individuals present highly variable clinical manifestations. anemia (SCA) is the most severe form, while SC hemoglobinopathy (HbSC) thought to be milder. Thus, we investigated manifestations and laboratory parameters by comparing each SCD genotype. We designed cross-sectional study including 126 SCA 55 HbSC steady-state. Hematological, biochemical inflammatory characterization was performed as well investigation...
Sickle cell anemia (SCA) is a hemolytic disease in which vaso-occlusion an important pathophysiological mechanism. The treatment based on hydroxyurea (HU), decreases leukocyte counts and increases fetal hemoglobin synthesis. Different types are thought to contribute vaso-occlusion. Nevertheless, the role of monocytes subsets remains unclear. We investigated frequencies blood their response HU therapy, testing ability express pro-inflammatory molecules tissue factor (TF). identified major...
In this study, we evaluate the association of different clinical profiles, laboratory and genetic biomarkers in patients with sickle cell anemia (SCA) hemoglobin SC disease (HbSC) attempt to characterize (SCD) genotypes.We conducted a cross-sectional study from 2013 2014 200 SCD individuals (141 SCA; 59 HbSC) analyzed demographic data population. addition, determined hematological, biochemical markers including βS-globin gene haplotypes 3.7 Kb deletion α-thalassemia (-α3.7Kb-thal), as well...
Sickle cell anemia (SCA) patients exhibit sub-phenotypes associated to hemolysis and vaso-occlusion. The disease has a chronic inflammatory nature that been also alterations in the lipid profile. This study aims analyze hematological biochemical parameters provide knowledge about SCA previously described suggest dyslipidemic sub-phenotype.A cross-sectional was conducted from 2013 2014, 99 steady state were enrolled. We assessed correlations associations with data investigated co-inheritance...
This study investigated the effects of hydroxyurea (HU) on hematological, biochemical and inflammatory parameters in children with sickle cell anemia (SCA) association βS haplotype α-thalassemia. We included 22 SCA who were followed for an average 14.5 months. Laboratory assessed by electronic methods, molecular analysis was PCR-RFLP allele-specific PCR. Results showed significant increases hemoglobin, HbF, hematocrit, MCV, MCH, glucose, HDL-C albumin levels, as well decreases MCHC AST WBC,...
The sickle cell disease (SCD) is a hemolytic genetic anemia characterized by free heme and hemoglobin release into intravascular spaces, with endothelial activation. Heme proinflammatory molecule able to directly activate vascular endothelium, thus, dysfunction are major chronic events described in SCD. aim of this study was evaluate the production nitric oxide synthase (eNOS), nitrite hypoxia inducible factor alpha (HIF-α) HUVECs (human umbilical vein cells) activated response simvastatin,...
This study investigated associations between SNPs in genes encoding metabolizing drug enzymes and laboratory parameters sickle cell anemia patients under hydroxyurea (SCA-HU + ). We evaluated hematologic biochemical by electronic methods PCR-RFLP multiplex PCR 35 SCA-HU 67 − patients. The HbS, total cholesterol, lactate dehydrogenase, aspartate aminotransferase, bilirubin fractions levels, leukocyte, eosinophil, monocyte, erythroblast counts were reduced (<mml:math...
Objective: Investigate the role of homocysteine (Hcy), Th17-related cytokines, and adhesion molecules in inflammatory state seen sickle cell anemia (SCA).Methods: We studied Hcy, interleukin (IL)-17, transforming growth factor β (TGF-β) cytokine levels 62 SCA patients, as well expression endothelial activation markers.Results: found significant associations between Hcy increased IL-17 TGF-β among a positive correlation soluble vascular cellular (sVCAM). individuals had raised when compared...
Abstract Background: Stroke is a severe clinical disorder in sickle cell disease (SCD), and few studies have evaluated transcranial Doppler (TCD) flow velocities hemoglobin SC (HbSC). The guidelines for stroke risk are based on evaluations anemia (SCA) or HbS/β thalassemia. Procedure: In this study, we compare cerebral blood patients with SCD stratified by genotypes. A total of 1,664 pediatric underwent TCD velocity screening, the time‐averaged maximum mean (TAMM) was determined middle...
Introduction . Clinical complications in sickle cell anemia (SCA) are heterogeneous and involve several molecules. It has been suggested that SCA individuals present a dyslipidemic phenotype lipid parameters associated with severe clinical complications, such as pulmonary hypertension. We sought to investigate associations between manifestations, well other laboratory population of pediatric patients. Methods Our cross-sectional evaluation included 126 patients steady state who were not...
Reference values for cerebral blood flow velocity (CBFV) in hemoglobin SC disease (HbSC) have not been established. We aimed to investigate associations between laboratory and genetic biomarkers associated with CBFV HbSC children. Sixty-eight children were included; was analyzed by transcranial Doppler, the time-averaged maximum mean (TAMMV) estimated. Hematological, biochemical, immunological, analyses performed. TAMMV negatively correlated red cell count (RBC) count, hemoglobin,...
Alpha-1 antitrypsin (AAT) is an inhibitor of neutrophil elastase and a member the serine proteinase (serpin) superfamily, little known about its activity in sickle cell disease (SCD). We hypothesize that AAT may undergo changes SCD because high oxidative stress inflammation associated with disease. have found levels patients compared to controls, while mutant genotypes SERPINA1 gene had decreased levels, both groups. showed negative correlation red blood cells, hemoglobin (Hb), hematocrit,...
Priapism is a urologic emergency characterized by an uncontrolled, persistent and painful erection in the absence of sexual stimulation, which can lead to penile fibrosis impotence. It highly frequent sickle cell disease (SCD) associated with hemolytic episodes. Our aim was investigate molecules that may participate regulation vascular tone. Eighty eight individuals SCD were included, whom thirty-seven reported history priapism. found be alterations laboratory biomarkers, as well lower...
Differences in hydroxyurea response sickle cell anemia may arise due to a series of factors with genetic appearing be predominant. This study aims investigate the effects single nucleotide polymorphisms genes encoding drug-metabolizing enzymes and solute carriers on response, patients anemia. For that purpose, total number 90 were recruited, 45 undergoing treatment, while not under treatment. Association analyses performed between CYP3A4 (rs2740574), CYP2D6 (rs3892097), CAT (rs7943316...
Transforming growth factor beta (TGF-β) is a cytokine with important involvement in biological processes related to the pathogenesis of sickle cell disease (SCD), including endothelial and vascular dysfunction, inflammation, hematopoietic homeostasis. This study aimed at investigating associations between levels TGF-β1 classical laboratory biomarkers inflammatory mediators, as well tissue inhibitor metalloproteases-1 (TIMP-1) matrix metalloproteinase-9 (MMP-9), pediatric patients (n = 123)...
Sickle cell anemia (SCA) patients have vascular complications, and polymorphisms in endothelin-1 (ET-1) endothelial nitric oxide synthase (eNOS) genes were associated with ET-1 disturbance. We investigate the association of 5665G>T eNOS -786T>C soluble adhesion molecules (sVCAM-1 sICAM-1), biochemical markers, medical history. studied 101 SCA patients; carriers minor allele (C) had highest levels sVCAM-1, more occurrence acute chest syndrome (ACS). The multivariate analysis suggested...
We investigated the nasopharynx and oropharynx microbiota in sickle cell disease (SCD) to identify microorganisms, antibiotic sensitivity, prevalent serotypes, association of with laboratorial markers. Oropharynx/nasopharynx secretions were 143 SCD children aging 6 months 17 years. Pathogens isolated using standard procedures, markers performed by automated methods. Staphylococcus aureus (S. aureus) was from 64 respectively. Streptococcus pneumoniae pneumoniae) eight patients. Serotypes S....
Brazilian Quilombolas are communities composed of African-derived populations that have their territories guaranteed by the Constitution. The present study investigated hemoglobin (Hb) variants among these population groups. This was conducted in a total 2843 individuals Quilombola Bahia, Pará, and Piauí states. All participants had Hb profiles evaluated. S (HBB: c.20A>T) variant described all studied localities. However, Bahia State highest frequency C c.19G>A) variant; from higher D-Punjab...