A5016286442- Bioactive Compounds and Antitumor Agents
- Vitamin C and Antioxidants Research
- Phytochemistry and Biological Activities
- Plant-Derived Bioactive Compounds
- Cancer therapeutics and mechanisms
- Drug-Induced Hepatotoxicity and Protection
- Bee Products Chemical Analysis
- Essential Oils and Antimicrobial Activity
- Electrospun Nanofibers in Biomedical Applications
- Berberine and alkaloids research
- Synthesis and Biological Evaluation
- Complementary and Alternative Medicine Studies
- Antioxidant Activity and Oxidative Stress
- Pesticide Exposure and Toxicity
- biodegradable polymer synthesis and properties
- Phytochemistry Medicinal Plant Applications
- Plant Toxicity and Pharmacological Properties
- Genomics, phytochemicals, and oxidative stress
- Heavy Metals in Plants
- Insect and Pesticide Research
- Pesticide and Herbicide Environmental Studies
- Medicinal Plants and Bioactive Compounds
- Natural product bioactivities and synthesis
- Bioactive Natural Diterpenoids Research
- Fluoride Effects and Removal
Universidade Federal do Paraná
2016-2024
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2023
Hospital de Clínicas Universidade Federal do Paraná
2017-2020
Universidade Federal do Rio Grande do Sul
2016-2019
Universidade Federal de Santa Catarina
2006-2018
UCLouvain
2014
ABSTRACT Acridine Orange is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles (AVOs). Its metachromatic shift to red fluorescence concentration-dependent and, therefore, fluoresces AVOs, such as autolysosomes. This makes staining quick, accessible reliable method assess the volume of which increases upon autophagy induction. Here, we describe ratiometric analysis using Orange, considering red-to-green intensity ratio (R/GFIR) quantify flow...
Standard therapy for acute promyelocytic leukaemia (APL) includes retinoic acid (all-trans (ATRA)), which promotes differentiation of blasts. Although co-administration arsenic trioxide (ATO) with ATRA has emerged as an effective option to treat APL, the molecular basis this effect remains unclear. Four cancer human models (HL60, THP-1, NBR4 and NBR4-R2 cells) were treated either ATO alone or plus ATRA. Cancer cell survival was monitored by trypan blue exclusion DEVDase activity assays. Gene...
Although stingless bees are widespread in tropical and subtropical regions worldwide, their by-products, including propolis, rarely used. In this study, we aimed to chemically analyze investigate the potential of mid-infrared (MIR) spectroscopy chemometric analyses for authentication propolis. The content phenolic compounds total flavonoids were used as quality parameters according guidelines Brazilian legislation. Attenuated reflection-infrared (ATR-IR) was performed, a model developed...
Bidens pilosa L. is a plant considered medicinal by some South American cultures. It contains polyacetylenes which may be the constituents responsible for its antitumor activity. Extracts obtained hydroethanol maceration (HCE) and supercritical fluid extraction (SFE) were monitored activity presence of in constitution. Both extracts killed concentration-dependently MCF-7 cells culture, although SFE extract presented superior cytotoxic The IC50 = 437 (428–446) μg/mL 24 h incubation,...
The aim of this study was to enhance the understanding antitumor mechanism 1,4-naphthoquinones and ascorbate. Juglone, phenylaminonaphthoquinone-7, 9 (Q7/Q9) were evaluated for effects on CT-DNA DNA cancer cells. Evaluations in MCF-7 cells are damage, ROS levels, viability, proliferation. Proteins from lysates immunoblotted verifying PARP integrity, γ H2AX, pAkt. Antitumor activity measured Ehrlich ascites carcinoma-bearing mice. same markers molecular toxicity assessed vivo ....
Bidens pilosa is a plant used by Amazonian and Asian folks for some hepatopathies. The hydroethanol crude extract three fractions were assessed antioxidant hepatoprotective effects. Higher levels of scavenger activity on the 1,1-diphenyl-2-picrylhydrazyl radical, inhibition deoxyribose oxidation lipid peroxidation in vitro detected ethyl acetate fraction (IC(50)~4.3-32.3 µg/ml) followed (IC(50)~14.2-98.0 µg/ml). fraction, again extract, showed high contents total soluble polyphenols (3.6±0.2...
Although cancer is a chronic disease, most of the in vitro experiments to assess effectiveness intervention are performed hours or few days. Moreover, none available methodologies measure cell proliferation adapted provide information about growth kinetic during and after treatment. Thus, objective this work guide long-term changes population size be used mainly research. Cumulative doubling (CPD) graphs based on counting for tumor volume vivo assays were calculate four parameters: relative...
Induced pluripotent stem cells (iPSC) have a great potential, but their clinical application depends on finding strategies to abolish tumorigenic potential. The use of Oct4, Sox2, Klf4, c-Myc and Nanog generate iPSC demonstrated the already known importance these genes maintain stemness. Therefore, presence is responsible for iPSC-derived teratomas. Similar iPSC, P19 teratocarcinoma cell line also has characteristics embryonic carcinoma ability differentiate into many types. We separately...
Background: This study investigated the hepatoprotective effect and antioxidant properties of phloroacetophenone (2′,4′,6′-trihydroxyacetophenone – THA), an acetophenone derived from plant Myrcia multiflora.Material & Method: The free radical scavenging activity in vitro induction oxidative hepatic damage by carbon tetrachloride (CCl4) (0.5 ml/kg, i.p.) were tested male Swiss mice (25±5 g).Results: compound exhibited effects on FeCl2–ascorbate-induced lipid peroxidation (LPO) mouse liver...
Poly(ε-caprolactone) (PCL) is commonly used in devices for tissue reconstruction due to its biocompatibility and suitable mechanical properties. However, high crystallinity hydrophobicity do not favor cell adhesion difficult polymer bioresorption. To improve these characteristics, the development of engineered scaffolds regeneration, based on poly(globalide-co-ε-caprolactone) (PGlCL) covalently bonded with N-acetylcysteine (PGlCL-NAC) was proposed. The were obtained from blends PCL...
Superparamagnetic iron oxide nanoparticles (SPIONs) have their use approved for the diagnosis/treatment of malignant tumors and can be metabolized by organism. To prevent embolism caused these nanoparticles, they need to coated with biocompatible non-cytotoxic materials. Here, we synthesized an unsaturated copolyester, poly (globalide-co-ε-caprolactone) (PGlCL), modified it amino acid cysteine (Cys) via a thiol-ene reaction (PGlCLCys). The Cys-modified copolymer presented reduced...
Naphthoquinones interact with biological systems by generating reactive oxygen species (ROS) that can damage cancer cells. The cytotoxicity and the antitumor activity of 3‑acyl‑2‑phenylamino‑1,4‑naphthoquinones (DPB1‑DPB9) were evaluated in MCF7 human breast cell line male Ehrlich tumor‑bearing Balb/c mice. DPB4 was most cytotoxic derivative against cells (EC50 15 µM) DPB6 least one 56 µM). 1,4‑naphthoquinone derivatives able to cause DNA promote fragmentation as shown plasmid cleavage assay...
Pharmacological doses of ascorbate were evaluated for its ability to potentiate the toxicity sodium orthovanadate (Na(3)VO(4)) in tumor cells. Cytotoxicity, inhibition cell proliferation, generation ROS and DNA fragmentation assessed T24 Na(3)VO(4) was cytotoxic against cells (EC(50)=5.8 μM at 24 h), but presence (100 μM) EC(50) fell 3.3 μM. plus caused a strong proliferation (up 20%) increased (4-fold). did not directly cleave plasmid DNA, this aspect no synergism found occurring between...
The antioxidant potential of crude extracts and fractions from leaves Ouratea parviflora, a Brazilian medicinal plant used for the treatment inflammatory diseases, was investigated in vitro through scavenging radicals 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), hydroxyl radical (HO•), superoxide anion (O2•−), lipid peroxidation rat liver homogenate. extract (CEOP) hydro-alcoholic fraction (OP4) showed strong inhibitory activity toward induced by tert-butyl peroxide (IC50 = 2.3 ± 0.2 1.9 0.1...
Evidence point out promising anticancer activities of Dihydropyrimidinones (DHPM) and organoselenium compounds. This study aimed to evaluate the cytotoxic antiproliferative potential DHPM-derived selenoesters (Se-DHPM), as well their molecular mechanisms action. Se-DHPM cytotoxicity was evaluated against cancer lines (HeLa, HepG2, MCF-7) normal cells (McCoy). HepG2 clonogenic assay allowed verifying effects. The propidium iodide/ orange acridine fluorescence readings showed type cell death...