A5019118107- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Alcohol Consumption and Health Effects
- Escherichia coli research studies
- Cannabis and Cannabinoid Research
- Isotope Analysis in Ecology
- Time Series Analysis and Forecasting
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Chromatography
- Polyamine Metabolism and Applications
- Drug Transport and Resistance Mechanisms
- Diet and metabolism studies
- Chemical Reactions and Isotopes
- Biochemical Analysis and Sensing Techniques
- Cancer Research and Treatments
- Vitamin C and Antioxidants Research
- Bacterial Genetics and Biotechnology
- Lysosomal Storage Disorders Research
- Infant Nutrition and Health
- Nanoparticle-Based Drug Delivery
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Enzyme function and inhibition
Uppsala University
2018-2025
Science for Life Laboratory
2018-2025
ETH Zurich
2025
Abstract Even though raw mass spectrometry data is information rich, the vast majority of underutilized. The ability to interrogate these rich datasets handicapped by limited capability and flexibility existing software. We introduce Mass Spec Query Language (MassQL) that addresses issues enabling an expressive set patterns be queried directly from data. MassQL open-source query language for flexible spectrometer manufacturer-independent mining MS envision flexibility, scalability, ease use...
New sulfatase-based assay for the selective identification of sulfate esters in human urine and fecal samples.
Abstract While metabolites derived from gut microbiota metabolism have been linked to disease development in the human host, chemical tools required for their detailed analysis and discovery of biomarkers are limited. A unique multifunctional probe mass spectrometric analysis, which contains p ‐nitrocinnamyloxycarbonyl as a new bioorthogonal cleavage site has designed synthesized. Coupled magnetic beads, this allows straightforward extraction samples release under mild conditions. This...
Sleep has evolved as a universal core function to allow for restorative biological processes. Detailed knowledge of metabolic changes necessary the sleep state in brain is missing. Herein, we have performed an in-depth analysis four mouse regions and uncovered region-specific circadian variations. Metabolites linked oxidative stress were altered during including acylcarnitines, hydroxylated fatty acids, phenolic compounds, thiol-containing metabolites. These findings provide molecular...
N-Acetyltransferases play critical roles in the deactivation and clearance of xenobiotics, including clinical drugs. NAT2 has been classified as an arylamine N-acetyltransferase that mainly converts aromatic amines, hydroxylamines, hydrazines. Herein, we demonstrate human also acetylates aliphatic endogenous amines. Metabolomic analysis chemical synthesis revealed increased intracellular concentrations mono- diacetylated spermidine cell lines expressing rapid compared to slow acetylator...
To advance the understanding of inflammatory bowel disease [IBD] pathophysiology, we compared mucosal and plasma metabolomes between new-onset paediatric IBD patients symptomatic non-IBD controls, correlated inflammation markers characteristics with altered metabolites. Paired colonic ileal biopsies from 67 treatment-naïve children incident Crohn's [CD; n = 47], ulcerative colitis [UC; 9], controls [n 11] were analysed using ultra-performance liquid chromatography-mass spectrometry...
Multidrug-resistant microorganisms have become a major public health concern around the world. The gut microbiome is gold mine for bioactive compounds that protect human body from pathogens. We used multi-omics approach integrated whole-genome sequencing (WGS) of 74 commensal isolates with metabolome analysis to discover their metabolic interaction Salmonella and other antibiotic-resistant evaluated differences in functional potential these selected based on WGS annotation profiles....
Comprehensive metabolomics requires robust and efficient analytical techniques capable of addressing the chemical diver-ity, complexity, high sample throughput demands characteristic large-scale studies. We introduce a rapid, mixed-mode liquid chromatography method that uniquely integrates anion exchange hydrophobic interactions within single stationary phase. Employing an optimized ternary gradient, our achieves comprehensive separation diverse metabolite classes over wide range polarities...
The gut microbiome converts dietary compounds that are absorbed in the gastrointestinal tract and further metabolized by human host. Sulfated metabolites a major compound class derived from this co-metabolism have been linked to disease development. In present multidisciplinary study, we investigated urine samples intervention study with 22 individuals collected before after consumption of polyphenol rich breakfast. These were analyzed utilizing our method combining enzymatic metabolite...
Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and adults endemic areas. Gene regulation ETEC during growth
Abstract Glucuronidation is the most common phase II modification and plays an important role in human clearance metabolism. Glucuronidated metabolites have also been linked to disease development microbiota–host co‐metabolism. Although many of these compounds identified, total number unknown glucuronides their impact on host's physiology can only be estimated. Herein, we describe combination untargeted metabolomics analysis enzymatic metabolic conversion for selective detection glucuronide...
Sleep is a state in which important restorative and anabolic processes occur. Understanding changes of these metabolic during the circadian rhythm brain crucial to elucidate neurophysiological mechanisms for sleep function. Investigation amino acid modifications dipeptides has recently emerged as valuable approach profiling central nervous system. Nonetheless, very little known about effects on levels analogues. In present study, we examined regional sleep-induced alterations selective...
Metabolomics analysis of biological samples is widely applied in medical and natural sciences. Assigning the correct chemical structure metabolite identification process required to draw conclusions still remains a major challenge this research field. Several tandem mass spectrometry (MS/MS) fragmentation spectra libraries have been developed that are either based on computational methods or authentic libraries. These limited due high number structurally diverse metabolites, low commercial...
The human body has evolved to remove xenobiotics through a multistep clearance process. Non-endogenous metabolites are converted series of phase I and different II enzymes into compounds with higher hydrophilicity. These important for diverse research fields such as toxicology, nutrition, biomarker discovery, doping control, microbiome metabolism. One the challenges in these been investigation two major modifications, sulfation glucuronidation, corresponding unconjugated aglycon...
Abstract While metabolites derived from gut microbiota metabolism have been linked to disease development in the human host, chemical tools required for their detailed analysis and discovery of biomarkers are limited. A unique multifunctional probe mass spectrometric analysis, which contains p ‐nitrocinnamyloxycarbonyl as a new bioorthogonal cleavage site has designed synthesized. Coupled magnetic beads, this allows straightforward extraction samples release under mild conditions. This...
Sulfation of metabolites is the second highest phase II modification in humans, which plays a critical role xenobiotics clearance process and gut microbiota-host co-metabolism. Besides main function to remove from body, sulfated have also been linked inflammation, bacterial pathogenesis metabolic disorders. A better understanding how these impact human body has turned into an important research area. Analytical methods for selective identification this metabolite class are scarce. We...
Metabolic microbiome interaction with the human host has been linked to physiology and disease development. The elucidation of this interspecies metabolite exchange will lead identification beneficial metabolites modulators. Their discovery quantitative analysis requires development specific tools compound classes. Sulfated are considered a readout for co-metabolism their host. This class is part phase II clearance process xenobiotics main focus in drug or doping metabolism also includes...
Loss of heterozygosity (LOH) is a hallmark feature cancer genomes that reduces allelic variation, thereby creating tumor specific vulnerabilities which could be exploited for therapeutic purposes. We previously reported loss drug metabolic arylamine N-acetyltransferase 2 (NAT2) activity following LOH at 8p22 targeted collateral lethality anticancer therapy in colorectal (CRC). Here, we report novel compound CBK034026C exhibits toxicity towards CRC cells with high NAT2 activity. Connectivity...
Abstract N ‐Acetyltransferases play critical roles in the deactivation and clearance of xenobiotics, including clinical drugs. NAT2 has been classified as an arylamine ‐acetyltransferase that mainly converts aromatic amines, hydroxylamines, hydrazines. Herein, we demonstrate human also acetylates aliphatic endogenous amines. Metabolomic analysis chemical synthesis revealed increased intracellular concentrations mono‐ diacetylated spermidine cell lines expressing rapid compared to slow...
Abstract Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and adults endemic areas. Gene regulation ETEC during growth vitro vivo needs to be further evaluated, here we describe the full transcriptome metabolome from mid-logarithmic stationary phase rich medium (LB medium). We identified specific genes pathways subjected rapid transient alterations gene expression metabolite production transition between logarithmic growth. The late exponential different...