A5000617617- SARS-CoV-2 and COVID-19 Research
- Lung Cancer Treatments and Mutations
- Mast cells and histamine
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- COVID-19 Impact on Reproduction
- Lung Cancer Research Studies
- Allergic Rhinitis and Sensitization
- PI3K/AKT/mTOR signaling in cancer
- HER2/EGFR in Cancer Research
- Drug-Induced Adverse Reactions
- Viral gastroenteritis research and epidemiology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- RNA modifications and cancer
- Food Allergy and Anaphylaxis Research
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- Angiogenesis and VEGF in Cancer
- Bone and Dental Protein Studies
- Cytokine Signaling Pathways and Interactions
- Bone Metabolism and Diseases
- Pluripotent Stem Cells Research
- Endometrial and Cervical Cancer Treatments
University of Hong Kong
2005-2025
Chinese University of Hong Kong
2012-2024
Hong Kong Polytechnic University
2022
Grantham Hospital
2009
Queen Mary Hospital
2009
Zhengzhou University
2005
The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) fusion gene resulting from the chromosome inversion inv(2)(p21;p23) recently was identified in nonsmall cell lung cancer (NSCLC). authors of this study investigated frequency, genetic and clinicopathologic profiles EML4-ALK Chinese patients with NSCLC.EML4-ALK 266 resected primary NSCLC, including adenocarcinomas (AD), lymphoepithelioma-like carcinomas, squamous mucoepidermoid adenosquamous by reverse...
Abstract Purpose: This study evaluated the mutational profile of epidermal growth factor receptor (EGFR) and KRAS in non–small cell lung cancers Hong Kong determined their relation with smoking history other clinicopathologic features. Experimental Design: Mutational exons 18 to 21 EGFR codons 12, 13, 61 were 215 adenocarcinomas, 15 squamous (SCC), 11 EBV-associated lymphoepithelioma-like carcinomas (LELC). Results: mutations prevalent adenocarcinomas (115 215), uncommon LELC (1 11), not...
STAT6 (signal transducer and activator of transcription 6) is a factor that plays central role in the pathophysiology allergic inflammation. We have identified 16 patients from 10 families spanning three continents with profound phenotype early-life onset immune dysregulation, widespread treatment-resistant atopic dermatitis, hypereosinophilia esosinophilic gastrointestinal disease, asthma, elevated serum IgE, IgE-mediated food allergies, anaphylaxis. The cases were either sporadic (seven...
Abstract Epidermal growth factor receptor (EGFR) mutations are common in lung adenocarcinomas, especially from nonsmoking women of Asian descent. We have previously shown EGFR occur >70% adenocarcinoma nonsmokers our population with a complex mutational profile, including 13% double mutations. In this study, we investigated the vitro gefitinib response four mutants identified untreated patients, Q787R+L858R, E709A+G719C, T790M+L858R, and H870R+L858R. The phosphorylation profiles...
To assess, in tissue microarray (TMA), the proliferative activity of endometrial carcinoma using one minichromosome maintenance (MCM) proteins (MCM7), and to explore its potential value for prognosis. MCM are essential eukaryotic DNA replication have recently been used define compartments human tissues.Immunohistochemistry MCM7 Ki67 was performed on TMAs constructed from 212 cases carcinoma. expression quantified according extent nuclear staining. An analysis carried out association between...
Introduction Two doses of inactivated SARS-CoV-2 vaccine CoronaVac cannot elicit high efficacy against symptomatic COVID-19, especially the Omicron variant, but that can be improved by a third dose in adults. The use adolescents may supported immunobridging studies absence data. Methods With an design, our study (NCT04800133) tested non-inferiority binding and neutralizing antibodies T cell responses induced healthy (N=94, median age 14.2 years, 56% male) compared to adults (N=153, 48.1 44%...
Abstract Background Children with chronic kidney diseases (CKD) are at risk of severe complications after SARS-CoV-2 infection and recommended to receive vaccine boosters. Although COVID-19 boosters effective in providing immune responses among healthy children, data on the use a fourth dose children CKD limited. Methods We prospectively investigated immunogenicity safety BNT162b2 CKD. Dosages were 0.1 mL 0.3 for aged 5–11 years 11–18 years, respectively. Humoral cellular was assessed...
Abstract Molecular-targeted therapy using tyrosine kinase inhibitors against epidermal growth factor receptor (EGFR) is an effective for non–small cell lung cancer that harbor EGFR mutations. This study aimed to investigate the role of Src, a close associator, as drug target in NSCLC cells with different genomic statuses. Src inhibition was achieved 4-(4′-Phenoxyanilino)-6,7-dimethoxyquinazolinee (SKI-1) and specificity action verified by RNA interference. The results showed SKI-1 induced...
Our study (NCT04800133) aimed to determine the safety and immunogenicity in patients with IEIs receiving a 3-dose primary series of mRNA vaccine BNT162b2 (age 12+) or inactivated whole-virion CoronaVac 3+) Hong Kong, including Omicron BA.1 neutralization, nonrandomized manner. Intradermal vaccination was also studied. Thirty-nine were vaccinated, 16 homologous intramuscular 0.3ml 17 0.5ml CoronaVac. Two received 3 doses intradermal CoronaVac, 4 2 third dose BNT162b2. No concerns identified....
Agonists of CCR1 contribute to hypersensitivity reactions and atherosclerotic lesions, possibly via the regulation transcription factor STAT3. was demonstrated use pertussis toxin-insensitive Gα(14/16) stimulate phospholipase Cβ NF-κB, whereas both Gα(14) Gα(16) are also capable activating The coexpression in human THP-1 macrophage-like cells suggests that may induce STAT3 activation. In this study, we a agonist, leukotactin-1 (CCL15), could indeed Tyr(705) Ser(727) phosphorylation G...
Abstract The high effectiveness of the third dose BNT162b2 in healthy adolescents against Omicron BA.1 has been reported some studies, but immune responses conferring this protection are not yet elucidated. In analysis, our study (NCT04800133) aims to evaluate humoral and cellular wild-type (BA.1, BA.2 and/or BA.5) SARS-CoV-2 before after a adolescents. At 5 months 2 doses, S IgG, IgG Fc receptor-binding, neutralising antibody waned significantly, antibodies remained detectable all tested...
Mast cells are pivotal in the pathogenesis of allergy and inflammation. In addition to classical IgE-dependent mechanism involving crosslinking high-affinity receptor for IgE (FcεRI), mast also activated by Toll-like receptors (TLRs) which at center innate immunity. this study, we demonstrated that response LAD2 (a human cell line) anti-IgE was altered presence TLR2 agonists peptidoglycan (PGN) tripalmitoyl-S-glycero-Cys-(Lys)4 (Pam3CSK4). Pretreatment PGN Pam3CSK4 inhibited induced calcium...
Abstract Recurrent incidents of economically motivated adulteration have long-lasting and devastating effects on public health, economy, society. With the current food authentication methods being target-oriented, lack an effective methodology to detect unencountered adulterants can lead next melamine-like outbreak. In this study, ensemble machine-learning model that help unprecedented without looking for specific substances, is, in a non-targeted approach, is proposed. Using raw milk as...
Patients with severe kidney diseases are at risk of complications from COVID-19; however, little is known about the effectiveness COVID-19 vaccines in children and adolescents diseases.We investigated immunogenicity safety an accelerated 3-dose primary series vaccination among 59 pediatric patients chronic disease (CKD) (mean age 12.9 years; 30 male) or without immunosuppression, dialysis, transplant. Dosage was 0.1 ml BNT162b2 to those aged 5 11 years, 0.3 18 years.Three doses either...
Background: Mast cells are the major effector that mediate IgE-dependent allergic reactions via high-affinity IgE receptors. We sought to use integrated network analysis identify genomic biomarkers associated with high response in IgE-mediated activation of primary human mast cells. Methods: Primary cell cultures derived from a cohort 262 normal donors were categorized into High, Average and Low responder groups according their profiles. Microarray followed by qPCR was used genes...
Abstract Background Optimising the immunogenicity of COVID-19 vaccines to improve their protection against disease is necessary. Fractional dosing by intradermal (ID) administration has been shown be equally immunogenic as intramuscular (IM) for several vaccines, but ID inactivated whole severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at full dose unknown. This study (NCT04800133) investigated superiority antibody and T-cell responses full-dose CoronaVac over IM in adolescents....
Mast cells play important roles in innate immunity through their activation via toll-like receptors (TLRs) but also contribute to neuroimmunological responses and inflammation by the neuropeptide substance P (SP) Gα i/o proteins. This study aims compare effects of TLR2 agonists peptidoglycan (PGN) tripalmitoyl-S-glycero-Cys-(Lys)4 (Pam3CSK4) on SP-induced human mast cell activation. The line LAD2 was employed determined assays β-hexosaminidase, IL-8 intracellular calcium. did not cause...
<b><i>Background:</i></b><i></i> Mast cells are key immune effector which release chemokines, proteases, and other inflammatory mediators upon activation by immunological stimuli. The aim of this study was to investigate the effects co-releasing proteases on kinetics chemokine monocyte chemoattractant protein-1 (MCP-1) in immunoglobulin E (IgE)-mediated human mast cells. <b><i>Methods:</i></b><i></i> Homogenous...
ABSTRACT Background Patients with kidney diseases are at risk of severe complications from COVID-19, yet little is known about the effectiveness COVID-19 vaccines in children and adolescents diseases. Methods We investigated immunogenicity safety an accelerated, 3-dose primary series among 64 pediatric chronic disease patients (mean age 12.2; 32 male) or without immunosuppression, dialysis, transplant. CoronaVac was given to those aged <5 years, 0.1ml BNT162b2 5-11 0.3ml 11-18 years....
There is no available data pertaining to humoral and cellular response against COVID-19 among young children with kidney diseases following inactivated vaccine.1Han B. Song Y. Li C. Yang W. Ma Q. Jiang Z. M. Lian X. Jiao Wang L. et al.Safety, tolerability, immunogenicity of an SARS-CoV-2 vaccine (CoronaVac) in healthy adolescents: a double-blind, randomised, controlled, phase 1/2 clinical trial.Lancet Infect Dis. 2021; 21: 1645-1653https://doi.org/10.1016/S1473-3099(21)00319-4Abstract Full...
Osteopontin (OPN) is an Arg-Gly-Asp (RGD)-containing extracellular matrix protein which upregulated in inflamed tissues and has been reported to modulate mast cell activities mice. Due the known heterogeneity among cells of different species important roles allergic reactions, we investigated effects human OPN (hOPN) on activities. Mature primary cultured (HCMC) were derived from peripheral blood CD34+ progenitors modulation their activation by soluble plate-bound immobilized hOPN examined...
Background: Two doses of inactivated SARS-CoV-2 vaccine CoronaVac cannot elicit high efficacy against symptomatic COVID-19, especially the Omicron variant, but that can be improved by a third dose in adults. The use adolescents may supported immunobridging studies absence data. Methods: With an design, our study (NCT04800133) aimed to evaluate binding and neutralizing antibodies T cell responses induced healthy for non-inferiority testing compared Responses wild-type (WT) BA.1 were...