A5050771225- Retinal Development and Disorders
- Retinal Diseases and Treatments
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Advanced Fluorescence Microscopy Techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Retinoids in leukemia and cellular processes
- bioluminescence and chemiluminescence research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Glycation End Products research
- Quantum Dots Synthesis And Properties
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Retinopathy of Prematurity Studies
- RNA Interference and Gene Delivery
Lions Eye Institute
2020-2025
The University of Western Australia
2020-2025
Abstract Adeno-associated viral (AAV) vector-mediated retinal gene therapy is an active field of both pre-clinical as well clinical research. As with other targets, novel bioengineered AAV variants developed by directed evolution or rational design to possess unique desirable properties, are entering translational programs. However, it becoming increasingly evident that predictive preclinical models required develop and functionally validate these AAVs prior studies. To investigate if, what...
Oxidative stress is pivotal in retinal disease progression, causing dysfunction various components. An effective antioxidant, such as probucol (PB), vital to counteract oxidative and emerges a potential candidate for treating degeneration. However, the challenges associated with delivering lipophilic drugs PB posterior segment of eye, specifically targeting photoreceptor cells, necessitate innovative solutions. This study uses formulation-based spray dry encapsulation technology develop...
Purpose: To validate the application of a known transgenic mouse line with green fluorescent cones (Chrnb4.EGFP) to study cone photoreceptor biology and function in health disease. Methods: Chrnb4.EGFP retinas containing GFP+ were compared without GFP transgene via immunohistochemistry, quantitative real-time polymerase chain reaction, electroretinograms, flow cytometry. The was backcrossed models degeneration, Pde6ccpfl1 Gnat2cpfl3, generating new lines Gnat2.GFP Pde6c.GFP, which also...
Inherited retinal diseases (IRDs) are a heterogeneous group of blinding disorders, which result in dysfunction or death the light-sensing cone and rod photoreceptors. Despite individual IRDs (Inherited disease) being rare, collectively, they affect up to 1:2000 people worldwide, causing significant socioeconomic burden, especially when cone-mediated central vision is affected. This study uses Pde6ccpfl1 mouse model achromatopsia, cone-specific loss IRD disease), investigate potential...
Abstract Adeno-associated viral (AAV) vector-mediated retinal gene therapy is an active field of both pre-clinical as well clinical research. As with other targets, novel bioengineered AAV variants developed by directed evolution or rational design to possess unique desirable properties, are entering translational programs. However, it becoming increasingly evident that predictive preclinical models required develop and functionally validate these AAVs prior studies. To this end, study...
Abstract Inherited retinal diseases (IRDs) are a heterogeneous group of blinding disorders, which result in dysfunction or death the light-sensing cone and rod photoreceptors. Despite individual IRDs being rare, collectively, they affect up to 1:2000 people worldwide, causing significant socioeconomic burden, especially when cone-mediated central vision is affected. This study uses Pde6c cpfl1 mouse model achromatopsia, cone-specific loss IRD, investigate potential gene-independent...