A5034637925- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Mycobacterium research and diagnosis
- Cancer therapeutics and mechanisms
- Carbohydrate Chemistry and Synthesis
- Antibiotics Pharmacokinetics and Efficacy
- Synthesis and biological activity
- Tuberculosis Research and Epidemiology
- Synthesis and Biological Activity
- Bacteriophages and microbial interactions
- Berberine and alkaloids research
- Bacterial Identification and Susceptibility Testing
- Marine Sponges and Natural Products
- Antimicrobial Peptides and Activities
- Phenothiazines and Benzothiazines Synthesis and Activities
- Bacterial biofilms and quorum sensing
- Computational Drug Discovery Methods
- Cancer-related gene regulation
- Bacterial Genetics and Biotechnology
- Synthetic Organic Chemistry Methods
- Quinazolinone synthesis and applications
- Antibiotic Use and Resistance
- TGF-β signaling in diseases
- Antimicrobial Resistance in Staphylococcus
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2025
National Health and Family Planning Commission
2016
Peking Union Medical College Hospital
2011-2013
Staphylococcus aureus can cause severe infections, including bacteremia and sepsis. The spread of methicillin-resistant (MRSA) highlights the need for novel treatment options. Sodium new houttuyfonate (SNH) is an analogue houttuynin, main antibacterial ingredient Houttuynia cordata Thunb. aim this study was to evaluate in vitro activity SNH its potential synergy with antibiotics against hospital-associated MRSA.A total 103 MRSA clinical isolates recovered two hospitals Beijing were evaluated...
Neo-actinomycins A and B (1 2), two new natural actinomycins featuring an unprecedented tetracyclic 5H-oxazolo[4,5-b]phenoxazine chromophore, were isolated from the marine-derived actinomycete Streptomyces sp. IMB094. Their structures elucidated by spectroscopic analyses. The presence of this ring system was proposed to originate a condensation between actinomycin D (3) with α-ketoglutarate pyruvate, respectively. Compound 1 showed potent cytotoxic activities against human cancer HCT116 A549...
Host cellular factor apolipoprotein B messenger RNA (mRNA)-editing enzyme catalytic polypeptide-like 3G (hA3G) is a cytidine deaminase that inhibits group of viruses including human immunodeficiency virus-1 (HIV-1). In the continuation our research on hA3G, we found hA3G stabilizing compounds significantly inhibited hepatitis C virus (HCV) replication. Therefore, this study investigated role in HCV Introduction external into HCV-infected Huh7.5 hepatocytes replication; knockdown endogenous...
A new griseofulvin derivative, 4′-demethoxy-4′-N-isopentylisogriseofulvin (1), three indole alkaloids, 2-demethylcyclopiamide E (2), 2-demethylsperadine F (3), and clopiamine C (4), five known metabolites (5–9) were isolated from Penicillium griseofulvum CPCC 400528. Compound 1 is the first reported analogue with an N-isopentane group example of a naturally occurring N-containing analogue. Their structures absolute configurations elucidated through extensive spectroscopic analyses,...
The increasing incidence of tigecycline resistance undoubtedly constitutes a serious threat to global public health. combination therapies had become the indispensable strategy against this threat. Herein, 11 clinical tigecycline-resistant Klebsiella pneumoniae which mainly has mutations in ramR , acrR or macB were collected for adjuvant screening. Interestingly, ML-7 hydrochloride (ML-7) dramatically potentiated activity. We further picked up five analogs and evaluated their synergistic...
Azvudine (FNC) is a novel cytidine analogue widely used in the treatment of AIDS and COVID-19 infectious disease. Previous studies have demonstrated its anticancer activity various cancer cell lines, including non-Hodgkin’s lymphomas lung adenocarcinoma lines. However, effects on hepatocellular carcinoma (HCC) underlying mechanisms remain unclear. This study aimed to investigate anti-epithelial-mesenchymal transition (anti-EMT) FNC evaluate potential application HCC treatment. We found that...
A pair of enantiomeric triketone–phloroglucinol hybrids, (+)- and (−)-myrtuspirone (1), featuring an unprecedented 3-isopropyl-3H-spiro[benzofuran-2,1′-cyclohexane] backbone, were isolated from the leaves Myrtus communis. The absolute configuration each enantiomer 1 was determined by X-ray diffraction chemical calculations. Furthermore, gram-scale total syntheses (±)-1 (−)-1 conducted in four steps using a Michael-N-iodosuccinimide (NIS)-mediated (3 + 2)-annulation reaction. Both exhibited...
Methicillin-resistant Staphylococcus aureus (MRSA) infection is a serious clinical threat, and D-Serine (D-Ser) showed significant sensitization effects on β-lactams against MRSA in our previous study. Quantitative PCR analysis found the elevated expression of dlt operon with D-Ser combination, which responsible for wall teichoic acid (WTA) modification involving D-Alanine (D-Ala). This study aims to verify effect WTA through pathway explore related bacteria. The DltA DltC were recombined,...
Azvudine (FNC) is a novel cytidine analogue that widely used in the treatment of infectious diseases such as AIDS and COVID-19. Previous studies have demonstrated its anticancer activity various cancer cell lines, including non-Hodgkin’s lymphomas lung adenocarcinoma lines. However, effects on hepatocellular carcinoma (HCC) underlying mechanisms remain unclear. This study aimed to investigate anti-epithelial–mesenchymal transition (anti-EMT) FNC evaluate potential application HCC treatment....
Protein arginine methyltransferase 1 (PRMT1) can catalyze the protein methylation by transferring methyl group from S-adenosyl-L-methionine (SAM) to guanidyl nitrogen atom of arginine, which influences a variety biological processes including epithelial–mesenchymal transition (EMT) and EMT-mediated mobility cancer cells. The upregulation PRMT1 is involved in diverse range cancer, such as lung there an urgent need develop novel potent inhibitors. In this article, series 2,5-substituted furan...
8-Acetoxycycloberberine (2) with a unique skeleton was first identified to display potent activity profile against Gram-positive bacteria, especially methicillin-resistant S. aureus (MRSA) minimum inhibitory concentration (MIC) values of 1–8 μg/mL, suggesting possible novel mechanism action bacteria. Taking 2 as the lead, 23 new 8-substituted cycloberberine (CBBR) derivatives including ether, amine, and amide were synthesized evaluated for their antibacterial effect. The structure–activity...
To combat escalating levels of antibiotic resistance, novel strategies are developed to address the everlasting demand for new antibiotics. This study aimed at investigating amicoumacin antibiotics from desert-derived
A new congener of chuangxinmycin (CM) was identified from Actinoplanes tsinanensis CPCC 200056. Its structure determined as 3-methylchuangxinmycin (MCM) by 1D and 2D NMR. MCM could be generated in vivo CM heterologous expression the vitamin B12-dependent radical SAM enzyme CxnA/A1 responsible for methylation 3-demethylchuangxinmycin (DCM) biosynthesis, indicating that perform iterative production. In vitro assays revealed significant activities CM, DCM, against Mycobacterium tuberculosis...
Mangrove actinomycetia have been proven to be one of the promising sources for discovering novel bioactive natural products. Quinomycins K (1) and L (2), two rare quinomycin-type octadepsipeptides without intra-peptide disulfide or thioacetal bridges, were investigated from Maowei Sea mangrove-derived Streptomyces sp. B475. Their chemical structures, including absolute configurations their amino acids, elucidated by a combination NMR tandem MS analysis, electronic circular dichroism (ECD)...
Introduction: The clinical and molecular characteristics of hypervirulent Klebsiella pneumoniae (hvKp) in various provinces China have been reported, however, there few reports Hebei Province, North China.
 Methodology: hvKp was identified by PCR amplification hypervirulence-related genes, the hypermucoviscous phenotype determined "string test", drug susceptibility analysis performed using VITEK® 2 Compact Bacterial Identification Monitoring System. Logistic regression used to identify...