Abstract Background Colorectal cancer (CRC) is one of the most common cancers all over world, and dysbiosis in gut microbiota may play a role colorectal carcinogenesis. Bacteroides fragilis can lead to tumorigenesis by changing signaling pathways, including WNT/β-catenin pathway. Therefore, present study, we investigated correlation between enterotoxigenic B. amount expression pathway genes involved CRC. Materials methods was determined 30 tumors adjacent healthy tissues qPCR method. Next,...

Introduction: The extensive drug-resistant (XDR) Pseudomonas aeruginosa ( P. ) causes a range of infections with high mortality rate, which inflicts additional costs on treatment. use nano-biotechnology-based methods in medicine has opened new perspective against bacteria. aim this study was to evaluate the effectiveness AgNO3 nanoparticles alone and conjugated imipenem (IMI) combat extensively . Methods: Antibiotic susceptibility carried out using disc diffusion method. Detection different...

Antibiotic resistance due to transferable genes is one of the most important concerns in Klebsiella pneumoniae isolated from nosocomial infections. Eighty-eight K. isolates were confirmed through biochemical methods. In addition, antimicrobial susceptibility testing was performed using a disc-diffusion method. Extended-spectrum β-lactamase production among screened double-disc synergism test, and identified PCR. The eight loci for multiple-locus variable number tandem repeat analysis (MLVA)...

Fusobacterium nucleatum has been recognized as an important key bacterium in the cause and spread of colorectal carcinogenesis. Nevertheless, clinical relevance F. cancer (CRC) its effect on immune factors tumor microenvironment have not fully elucidated.The frequency was measured 100 paired normal tissue specimens by TaqMan quantification Real-Time Polymerase Chain Reaction (qPCR). The mRNA expression levels cytokines (IL-6, IL-10, IL-12β, IL-17, TNF-α, TLR-2, TLR-4), miRNAs (miR-21,...

Background : The rise of drug‐resistant bacteria poses a significant challenge to global healthcare, underscoring the urgent need for new therapeutic interventions. In this study, ethanolic extracts Datura stramonium and Prosopis farcta , sourced from Northern Iran, were examined their antibacterial properties against Staphylococcus aureus Escherichia coli Acinetobacter baumannii Klebsiella pneumoniae Pseudomonas aeruginosa . Methods: activities medicinal in study using disc diffusion,...

Introduction:The incidence of urinary tract infections caused by Extended-Spectrum Beta Lactamase (ESBL) producing Escherichia coli (E.coli) strains due to long term and overuse broad-spectrum cephalosporine is on the rise.CTX beta-lactamase type, a beta-lactamase, has been expanding in many countries.The ctx gene harbored plasmid that spread between Enterobacteriaceae family, especially E. coli.The aim this study was determine pattern antimicrobial resistance investigate prevalent ESBL...

Background: Klebsiella pneumoniae as an opportunistic pathogen can be the cause of a range nosocomial and community - acquired infections. Many virulence factors help these bacteria overcome immune system various diseases. K1 K2 capsular antigens, also magA, wcaG, rmpA are well known K. factors. has been revealed to have ability acquire resistance many antibiotics, which treatment failure. Objectives: This study aimed at determining prevalence rmpA, Capsular type K1, K2, TEM, SHV in...

During the last decade, probiotic research has progressed considerably and significant advances have been made in selection characterization of specific strains. The most studied probiotics belong to genus Lactobacillus. In this study, 80 Lactobacillus spp. isolated from healthy women tolerated low pH were able grow presence bile salts. RAPD PCR technique resulted identification 38 different types. These isolates then evaluated based on adhesion capacity, antibiotic susceptibility tolerance...

Objective: Persistence is a subpopulation of bacteria that can tolerate high doses antibiotics by undergoing period dormancy. This study aimed to investigate the hypothetical role toxin/antitoxin systems in persister cell formation Klebsiella pneumoniae ATCC 13883 evaluating toxins type II expression. Methods: To determine number surviving cells, bacterial colonies were counted at 1, 3, 5, 7, and 24 h after treated with lethal dose kanamycin. The was then determined ( relE1 , relE2 hipA vapC...

Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal soft tissue that can be affecting bloodstream infections. Therefore, since has been reported in several studies from Iran, the current study, we have investigated molecular characteristics, typing, biofilm formation type E. collected UTI specimens.A total 173 isolates were collected. The susceptibility to all fourth generations...

Probiotics promote a healthy balance of gut bacteria and have many beneficial effects on human digestive physiology. Although, few side probiotics been reported. This study aimed to assess the safety five probiotic candidate Lactobacillus strains isolated from individuals by examining mutagenicity, genotoxicity, oral toxic effects.Five selected (SCPs) were evaluated for genotoxicity (Ames test with Salmonella typhimurium), in vitro mammalian chromosome aberration an vivo mouse micronucleus...

The use of probiotics is emerging as an innovative approach to managing oral health issues and mediating the immune system. current study assessed

Escherichia coli ST131 is a pandemic clone with high antibiotic resistance, and it major causative agent of urinary tract infection (UTI) bloodstream infections. This study evaluated the distribution expression virulence genes genotyping E. O25b/ST131 by Multi-locus variable number tandem repeat analysis (MLVA) method among UTI in patients at Tehran hospitals, Iran.A total 107 isolates were collected from patients. Polymerase chain reaction (PCR) amplification pabB gene was used to identify...

Background: CTX-M is the most prevalent and rapidly growing type of extended-spectrum β-lactamase (ESBL) family CTX-M1 common blaCTX-M. Objectives: The current study aimed at investigating genetic diversity CTX-M-1-producing Klebsiella pneumoniae circulating in Semnan, Iran evaluated by multilocus variable-number tandem repeat analysis (MLVA). Methods: A total 110 isolates K. were collected from different clinical samples. antibiotic suceptibility double disk synergy test determined...

Background & objective: Acinetobacter baumannii is an opportunistic pathogen with high pathogenic and antibiotic-resistance potential also considered as one of the main nosocomial agents, specifically in intensive care units (ICUs).It highly important to use molecular biology methods epidemiological studies, determine source infection, understand relationships distributional patterns pathogens.Therefore, current study aimed determining similar types A. species isolated from patients Tehran,...

Introduction: Plasmid-mediated quinolone resistance (PMQR) is a growing clinical concern throughout the world. The purpose of this study was to detect qnr-encoding genes and evaluate clonal relatedness qnr-positive Klebsiella pneumoniae isolates. Materials Methods: A total 88 K. isolates assessed which were obtained from Tehran hospital in Tehran, Iran. Bacterial identification administrated using standard laboratory methods. Quinolone determined Kirby-Bauer disk diffusion method according...

Background and Objectives: Endometriosis is defined as the presence of endometrial tissue outside uterine cavity. Pe- ripheral blood monocytes cells (PBMCs) may have altered function to some extent in women with endometriosis. Lactoba- cillus acidophilus a probiotic bacterium within human bodywith ability alleviating many inflammatory diseases. Here, we examined effect L. on PBMCs endometriosis patients.
 Materials Methods: In this study, peripheral samples were obtained from patients...

Background: Treatment of ocular infection by fungi has become a problematic issue, particularly in deep lesion cases, because the limited available antifungals and emerging resistance species. Objectives: The present study was designed for molecular identification studying antifungal susceptibility pattern fungi. Methods: Fifty-three fungal isolates, including Fusarium spp., Aspergillus yeast dematiaceous were collected. Initial each sample performed using routine mycological techniques....

Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples Imam Khomeini Hospital, Tehran, Iran 2018