A5040561744- Cholesterol and Lipid Metabolism
- Pharmacogenetics and Drug Metabolism
- Edible Oils Quality and Analysis
- Lipid Membrane Structure and Behavior
- Biochemical Analysis and Sensing Techniques
- Spectroscopy and Chemometric Analyses
- Consumer Attitudes and Food Labeling
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Protein Structure and Dynamics
- Essential Oils and Antimicrobial Activity
- Fatty Acid Research and Health
- Endoplasmic Reticulum Stress and Disease
- Meat and Animal Product Quality
- Antioxidant Activity and Oxidative Stress
- CRISPR and Genetic Engineering
- Steroid Chemistry and Biochemistry
- Metal-Catalyzed Oxygenation Mechanisms
- Receptor Mechanisms and Signaling
- Atherosclerosis and Cardiovascular Diseases
- Surfactants and Colloidal Systems
- Computational Drug Discovery Methods
- Phytochemicals and Antioxidant Activities
- Calcium signaling and nucleotide metabolism
- Microbial Inactivation Methods
University of Kansas
2016-2025
Michigan State University
2019-2024
University of Michigan
2017-2024
Quantitative BioSciences
2020-2023
Washington State University
2013-2018
Ann Arbor Center for Independent Living
2017-2018
Regional Centre of Advanced Technologies and Materials
2016
Palacký University Olomouc
2016
Ninewells Hospital
2016
University of Dundee
2016
Autophagy is a catabolic pathway required for the recycling of cytoplasmic materials. To define mechanisms underlying autophagy it critical to quantitatively characterize dynamic behavior factors in living cells. Using panel cell lines expressing HaloTagged from their endogenous loci, we analyzed abundance, single-molecule dynamics, and autophagosome association kinetics proteins involved biogenesis. We demonstrate that formation inefficient ATG2-mediated tethering donor membranes key...
<ns4:p>Cytochrome P450, a family of monooxygenase enzymes, is organized as catalytic metabolon, which requires enzymatic partners well environmental factors that tune its complex dynamic. P450 and reducing counterparts—cytochrome P450-reductase cytochrome <ns4:italic>b<ns4:sub>5</ns4:sub></ns4:italic>—are membrane-bound proteins located in the cytosolic side endoplasmic reticulum. They are believed to dynamically associate form functional complexes. Increasing experimental evidence signifies...
Autophagy is an important metabolic pathway that can non-selectively recycle cellular material or lead to targeted degradation of protein aggregates damaged organelles. Autophagosome formation starts with autophagy factors accumulating on lipid vesicles containing ATG9. These phagophores attach donor membranes, expand via ATG2-mediated transfer, capture cargo, and mature into autophagosomes, ultimately fusing lysosomes for their degradation. be activated by nutrient stress, example, a...
Abstract Although membrane environment is known to boost drug metabolism by mammalian cytochrome P450s, the factors that stabilize structural folding and enhance protein function are unclear. In this study, we use peptide‐based lipid nanodiscs “trap” boundaries of microsomal P450 2B4. We report first evidence CYP2B4 able induce formation raft domains in a biomimetic compound endoplasmic reticulum. NMR experiments were used identify quantitatively determine lipids present nanodiscs. A...
The average American consumes more than 50% of their total dietary energy from ultra-processed foods (UPFs). From a nutritional standpoint, as UPFs intake increases, fiber, vitamin, and mineral decrease. High consumption UPFs, mainly fast (FF) ready-to-eat (RTE) food items, emerges critical public health concern linking quality safety. In the present work, systematic database fatty acid composition most consumed in Midwest is reported. Saturated monounsaturated acids were predominant RTE...
Class III peroxidases (CIIIPRX) catalyze the oxidation of monolignols, generate radicals, and ultimately lead to formation lignin. In general, CIIIPRX genes encode a large number isozymes with ranges in vitro substrate specificities. order elucidate mode specificity these enzymes, we characterized one CIIIPRXs (PviPRX9) from switchgrass (Panicum virgatum), strategic plant for second-generation biofuels. The crystal structure, kinetic experiments, molecular docking, as well expression...
Cytochrome P450′s membrane interaction alters its spin equilibrium which is accompanied by a stronger affinity for cytochrome<italic>b</italic><sub>5</sub>.
Anticancer agent 6-mercaptopurine (6MP) has been in use since 1953 for the treatment of childhood acute lymphoblastic leukemia (ALL) and inflammatory bowel disease. Despite being available 60 years, several aspects 6MP drug metabolism pharmacokinetics humans are unknown. Molybdoflavoenzymes such as aldehyde oxidase (AO) xanthine (XO) have previously implicated this drug. In study, we investigated vitro to 6-thiouric acid (6TUA) pooled human liver cytosol. We discovered that is metabolized...
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing knock-in rates. We present a detailed pipeline using HDR donor plasmids containing antibiotic resistance cassettes for rapid selection gene-edited cells. Our protocol streamlines N- or C-terminal human cells, enabling plasmid preparation single cloning step.
Improving treatment options for head and neck squamous cell carcinoma (HNSCC) requires a deeper understanding of the tumor microenvironment, particularly cancer-associated fibroblasts (CAFs). We previously reported that HNSCC-derived FGF2/bFGF (fibroblast growth factor 2) triggers cytokine release from CAFs via secretory autophagy. Here, using transmission electron microscopy, live-cell imaging, immunofluorescence, we show CAF autophagosomes transport cargo, including IL6, to plasma membrane...
Cytochrome P450 reductase (CPR) is the redox partner for most human cytochrome enzymes. It also believed that CPR an integral membrane protein exclusively. Herein, we report that, contrary to this belief, can exist as a peripheral in absence of NADPH and will transition presence stoichiometric amounts or greater. All experiments were performed solid-supported cushioned lipid bilayer closely matched chemical composition endoplasmic reticulum served ER biomimetic. The phase characteristics...
Cytochrome P450-reductase (CPR) is a versatile NADPH-dependent electron donor located in the cytoplasmic side of endoplasmic reticulum. It an transferase that able to deliver electrons variety membrane-bound oxidative partners, including drug-metabolizing enzymes cytochrome P450s (P450). CPR also stoichiometrically limited compared its counterparts, and hypotheses have arisen about possible models can overcome stoichiometric imbalance, quaternary organization P450 diffusion-limited models....
Ultra-processed foods (UPFs) have gained substantial attention in the scientific community due to their surging consumption and potential health repercussions. In addition well-established poor nutritional profile, UPFs been implicated containing various dietary oxidized sterols (DOxSs). These DOxSs are associated with a spectrum of chronic diseases, including cardiometabolic conditions, cancer, diabetes, Parkinson's, Alzheimer's disease. this study, we present comprehensive database...
An in vitro observation of time-dependent inhibition (TDI) metabolic enzymes often results removing a potential drug from the pipeline. However, accepted method for predicting TDIs important metabolizing cytochrome P450 overestimates interaction potential. Better models that take into account complexities enzyme system will lead to better predictions. Herein we report use our previously described complex kinetics podophyllotoxin. Spectral characterization indicates an intermediate MI is...