A5020333855- Insect and Pesticide Research
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Insect Pest Control Strategies
- Bat Biology and Ecology Studies
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Enzyme-mediated dye degradation
- Animal Vocal Communication and Behavior
- RNA and protein synthesis mechanisms
- Environmental Toxicology and Ecotoxicology
- Plant Virus Research Studies
- Insect Utilization and Effects
- Viral gastroenteritis research and epidemiology
- Insect and Arachnid Ecology and Behavior
- Cellular Mechanics and Interactions
- Aquatic life and conservation
- Genetics, Aging, and Longevity in Model Organisms
- Turfgrass Adaptation and Management
- Dermatological diseases and infestations
Pennsylvania State University
2019-2025
Eastern University
2020-2021
Washington State University
2014-2019
Bluegrass Community and Technical College
2012-2014
University of Nebraska–Lincoln
2014
University of Kentucky
2013
Abstract Background The functional and evolutionary diversification of insect cytochrome P450s (CYPs) shaped the success insects. CYPs constitute one largest oldest gene superfamilies that are found in virtually all aerobic organisms. Because availability whole genome sequence well functioning RNA interference (RNAi), red flour beetle, Tribolium castaneum serves as an ideal model for conducting genomics studies. Although several T. had been functionally investigated our previous studies,...
Abstract The adaptation of herbivorous insects to their host plants is hypothesized be intimately associated with ubiquitous development resistance synthetic pesticides. However, not much known about the mechanisms underlying relationship between detoxification plant toxins and To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) its plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed reveal CPB transcriptome....
Background NADPH-cytochrome P450 reductase (CPR) plays a central role in cytochrome action. The genes coding for P450s are not yet fully identified the bed bug, Cimex lectularius. Hence, we decided to clone cDNA and knockdown expression of gene CPR which is suggested be required function all determine whether or involved resistance bugs insecticides. Methodology/Principal Findings full length lectularius (ClCPR) was isolated from deltamethrin resistant bug population (CIN-1) using combined...
Odorant binding proteins (OBPs) are small water-soluble mainly associated with olfaction, facilitating the transport of odorant molecules to their relevant receptors in sensillum lymph. While traditionally considered essential for recent research has revealed that OBPs engaged a diverse range physiological functions modulating chemical communication and defense. Over past 10 years, emerging evidence suggests play vital roles purifying perireceptor space from unwanted xenobiotics including...
Class III peroxidases (CIIIPRX) catalyze the oxidation of monolignols, generate radicals, and ultimately lead to formation lignin. In general, CIIIPRX genes encode a large number isozymes with ranges in vitro substrate specificities. order elucidate mode specificity these enzymes, we characterized one CIIIPRXs (PviPRX9) from switchgrass (Panicum virgatum), strategic plant for second-generation biofuels. The crystal structure, kinetic experiments, molecular docking, as well expression...
The two-spotted spider mite (Tetranychus urticae) is a highly destructive and economically significant pest in agricultural, horticultural, ornamental agroecosystems worldwide, including hop (Humulus lupulus) mint (Mentha spp.) fields the Pacific Northwest (PNW) region of United States. Repeated acaricide applications rotations have led to widespread resistance, resulting control failures. In this study, we investigated mechanisms resistance four different acaricides (bifenthrin, bifenazate,...
The Colorado potato beetle, Leptinotarsa decemlineata, is the primary defoliator of potatoes and notorious for its robust capability to develop resistance various insecticides used control it. As initial interface between environment insect olfactory system, odorant binding proteins (OBPs) solubilize transport hydrophobic molecules from sensillar lymph receptors. Recently, evidence has suggested that OBPs may also sequester excess harmful such as in perireceptor space, preventing them...
A genomic screen uncovers a link between molecular parallelism and the convergent evolution of superfast sound-producing muscles.
Abstract Neonicotinoid insecticides are used to manage spotted lanternfly (Lycorma delicatula (White); hereafter SLF), a recently introduced pest in the United States. Neonicotinoids can harm nontargets, such as pollinators potentially exposed via floral resources of treated plants. We quantified neonicotinoid residues whole flowers two SLF host plant species, red maple (Acer rubrum L. [Sapindales: Sapindaceae]) and tree-of-heaven (Ailanthus altissima (Mill.) Simaroubaceae]), with post-bloom...
The cotton bollworm, Helicoverpa armigera, is a worldwide polyphagous pest, causing huge economic losses in vegetable, and corn crops, among others. Owing to long-term exposure Bacillus thuringiensis (Bt) toxins, evolution of resistance has been detected this pest. As conservative effective neurotransmitter, dopamine (DA) an important role insect growth development. In study, we investigated the regulatory functions DA its associated non-coding RNA metamorphosis H. armigera.Expression...
PcpR is a LysR-type transcription factor from Sphingobium chlorophenolicum L-1 that responsible for the activation of several genes involved in polychlorophenol degradation. responds to polychlorophenols vivo. Here, we report crystal structures inducer-binding domain apo-form and binary complexes with pentachlorophenol (PCP) 2,4,6-trichlorophenol (2,4,6-TCP). Both X-ray isothermal titration calorimetry data indicated association two PCP molecules per PcpR, but only one 2,4,6-TCP molecule....
Porcine circovirus 2 is the primary agent responsible for inducing a group of associated diseases known as Circovirus Associated Diseases (PCVAD), which can have detrimental effects on production efficiency well causing significant mortality. The objective this study was to evaluate variation in viral replication, immune response and growth across pigs (n = 974) from different crossbred lines. approach used experimental infection with PCV2b strain at an average 43 days age. sequence isolate...
Arthropod Glutathione S-transferases (GSTs) constitute a large family of multifunctional enzymes that are mainly associated with xenobiotic or stress adaptation. GST-mediated adaptation takes place through direct metabolism sequestration xenobiotics, and/or indirectly by providing protection against oxidative induced exposure. To date, the roles GSTs in Colorado potato beetle (CPB), notorious agricultural pest plants within Solanaceae, have not been well studied. Here, we functionally...
Porcine circovirus type 2 (PCV2) is the etiological agent of a group associated diseases (PCVAD) that affect production efficiency and can lead to mortality. Using different crossbred lines pigs, we analyzed host genetic variation viral load, immune response weight change following experimental infection with PCV2b strain (n = 386). Pigs expressed in magnitude initiation viremia recorded weekly until 28 days post-infection. A higher load was correlated gain (r -0.26, P < 0.0001) presence...
The ocular albinism type 1 (OA1), a pigment cell-specific integral membrane glycoprotein, is member of the G-protein-coupled receptor (GPCR) superfamily that binds to heterotrimeric G proteins in mammalian cells. We aimed characterize physiological functions an insect OA1 from Lymantria dispar (LdOA1) employs regulation insecticide tolerance. In present study, we investigated roles LdOA1 response deltamethrin exposure both L. and Drosophila melanogaster. was expressed at lowest level during...
Broad-spectrum crop protection technologies, such as abamectin and bifenthrin, are globally relied upon to curb the existential threats from economic pests generalist herbivore Tetranychus urticae Koch (TSSM). However, rising cost of discovering registering new acaricides, particularly for specialty crops, along with increasing risk pesticide resistance development, underscores urgent need preserve efficacy currently registered acaricides. This study examined overall genetic mechanism...
Abstract The missense mutation R21H in striated muscle tropomyosin is associated with hypertrophic cardiomyopathy, a genetic cardiac disease and leading cause of sudden death young people. Tropomyosin adopts conformation coiled coil which critical for regulation contraction. In this study, we investigated the effects on coiled‐coil structure its interactions binding partners, tropomodulin leiomodin. Using circular dichroism isothermal titration calorimetry, found that profoundly destabilized...