A5049091838- Fungal Biology and Applications
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Microbial Natural Products and Biosynthesis
- Plant and fungal interactions
- Mycotoxins in Agriculture and Food
- Plant-Microbe Interactions and Immunity
- Protist diversity and phylogeny
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant Pathogens and Resistance
- Phytochemicals and Antioxidant Activities
- Biofuel production and bioconversion
- Biocrusts and Microbial Ecology
- Powdery Mildew Fungal Diseases
- Plant tissue culture and regeneration
- Enzyme-mediated dye degradation
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Polyamine Metabolism and Applications
- Pharmacological Effects of Natural Compounds
Fujian Agriculture and Forestry University
2016-2025
University of Guelph
2019-2020
Mitochondrial DNA (mtDNA) is a core non-nuclear genetic material found in all eukaryotic organisms, the size of which varies extensively eumycota, even within species. In this study, mitochondrial genomes six isolates Annulohypoxylon stygium (Lév.) were assembled from raw reads PacBio and Illumina sequencing. The diversity genomic structures, conserved genes, intergenic regions introns analyzed compared. Genome sizes ranged 132 kb to 147 contained same sets protein-coding, tRNA rRNA genes...
Mushroom-forming fungi are complex multicellular organisms that form the basis of a large industry, yet, our understanding mechanisms mushroom development and its responses to various stresses remains limited. The winter (Flammulina filiformis) is cultivated at commercial scale in East Asia species with preference for low temperatures. This study investigated fruiting body F. filiformis by comparing transcriptomes 4 developmental stages, compared genes 200-genome dataset identify conserved...
Abstract The selection of appropriate internal control genes (ICGs) is a crucial step in the normalization real-time quantitative PCR (RT-qPCR) data. Housekeeping are habitually selected for this purpose, despite accumulating evidence on their instability. We screened novel, robust ICGs mushroom forming fungus Volvariella volvacea . Nine commonly used and five newly were evaluated expression stability using RT-qPCR data eight different stages life cycle V. Three algorithms consistently...
Mushrooms are sources of food and medicine provide abundant nutrients bioactive compounds. However, most the edible mushrooms cannot be cultivated commercially due to limited understanding basidioma development. From winter mushroom ( Flammulina velutipes ; also known as Enokitake), one commonly mushrooms, we identified a novel transcription factor, PDD1, positively regulating PDD1 increases expression during Artificially increasing its promoted formation dramatically increased yield, while...
The initiation of sexual development in the important edible and medicinal mushroom Flammulina velutipes is controlled by special genes at two different, independent, mating type (MAT) loci: HD PR. We expanded our understanding F. system analyzing MAT loci from a series strains. locus houses homeodomain (Hd genes) on separated locations: sublocus HD-a HD-b. HD-b subloci contained strain-specific Hd1/Hd2 gene pairs, crosses between strains with different indicated role mating. function...
Hypomyces aurantius is a mycoparasite that causes cobweb disease, most serious disease of cultivated mushrooms. Intra-species identification vital for control, however the lack genomic data makes development molecular markers challenging. Small size, high copy number, and mutation rate fungal mitochondrial genome it good candidate intra inter species differentiation. In this study, H. H.a0001 was determined from DNA using Illumina sequencing. The roughly 72 kb shows all major features found...
Agaricus blazei Murill (ABM), a medicinal mushroom, has beneficial effects on various human metabolic diseases. The objective of this research was to evaluate the antioxidant and antidiabetic properties ABM extracts (ethanol extract ethyl acetate extract). activities ethanol (EE) (EA) were analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radical scavenging assays reducing power K3Fe(CN)6 in vitro. Moreover, EE EA...
Ganoderma resinaceum is a valuable Chinese medicine. This study aimed to investigate whether G. alcohol extract (GRAE) improves sleep, and analyze the potential mechanism. After 30 days of continuous administration GRAE at various doses, (1,000 mg/kg.bw) prolonged pentobarbital sodium-induced increased rate sleeping in mice treated with subthreshold dose sodium, shortened sleep latency. The brain was analyzed using UPLC-MS/MS RNA-sequencing. Metabolomics analysis revealed that 73 metabolites...
In the past, stakeholders paid more attention to quality and growth of edible fungi. There have been few studies on whether other microorganisms exist in sterilized media during process. this study, through periodic tracking, collection isolation, sequencing, data analysis, it was found that jade mushroom (Hypsizygus marmoreus) does not independently its life cycle. Ten different bacteria were isolated from five samples, four them did interfere with mycelial H. marmoreus cultivation media....
This study investigated the structure-activity relationships between SePEPs (selenium-enriched peptides) and PEPs (selenium-free compared antioxidant activities of PEPs. The results showed that exhibited higher activity than at same molecular weight, with weights 0-3500 Da exhibiting highest in vitro activity. Chelation selenium peptides led to a more compact structure increased particle density SePEPs. A spectroscopic analysis revealed new peaks redshifts SePEPs, along content hydrophobic...
Antifungal azoles inhibit ergosterol biosynthesis by interfering with lanosterol 14α-demethylase. In this study, seven upregulated and four downregulated genes in response to ketoconazole treatment were identified Neurospora crassa. Azole sensitivity test of knockout mutants for six ketoconazole-upregulated revealed that deletion only sterol C-22 desaturase ERG5 altered azoles: the erg5 mutant was hypersensitive but had no obvious defects growth development. The accumulated higher levels...
Abstract Background Flammulina filiformis (previously known as Asian F. velutipes ) is a popular commercial edible mushroom. Many bioactive compounds with medicinal effects, such polysaccharides and sesquiterpenoids, have been isolated identified from , but their biosynthesis regulation at the molecular level remains unclear. In this study, we sequenced genome of wild strain Liu355, predicted its biosynthetic gene clusters (BGCs) profiled expression these genes in cultivar strains different...
Flammulina filiformis , as one of the most popular edible fungi in East Asia, is produced an industrialized and standardized way. However, its monotonous variety product convergence have seriously restricted development industry. In this study, 11 cultivated strains 13 wild F. were collected from multiple regions China Japan performed genome sequencing. Together with data six previously released, total 23 dikaryons (formed by two monokaryons mating, can making fruiting body), 35...
ABSTRACT Antifungal azoles are widely used for controlling fungal infections. Fungi able to change the expression of many genes when they adapt azole stress, and increased some these can elevate resistance azoles. However, regulatory mechanisms behind transcriptional adaption in filamentous fungi poorly understood. In this study, we found that deletion transcription factor gene ccg-8 , which is known be a clock-controlled gene, made Neurospora crassa hypersensitive A comparative genome-wide...
, one of the most popular mushroom species in world, has been recognized as a useful model system to study biochemical and physiological aspects formation elongation fruit body. However, few reports have published on regulation fruiting body
Stipe elongation is an important process in the development of fruiting body and associated with commodity quality agaric fungi. In this study, F. filiformis was used as a model fungus to reveal function chromatin modifier gene containing JmjC domain stipe elongation. First, we identified family (FfJmhy) 3684 bp length open reading frame (ORF) filiformis. FfJmhy predicted have histone H3K9 demethylation function, specifically upregulated during rapid Further investigation revealed that...
The edible mushroom Volvariella volvacea is an important crop in Southeast Asia and predominantly harvested the egg stage. One of main factors that negatively affect its yield value rapid transition from to elongation stage, which has a decreased commodity shelf life. To improve our understanding changes during stipe development stage particular, we analyzed gene transcription tissue V. using 3′-tag based digital expression profiling. Stipe turned out be fairly complex with high numbers...