A5061993317- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Infective Endocarditis Diagnosis and Management
- Plant Pathogenic Bacteria Studies
- Pneumonia and Respiratory Infections
- Phenothiazines and Benzothiazines Synthesis and Activities
- Escherichia coli research studies
- Molecular Biology Techniques and Applications
- Veterinary medicine and infectious diseases
- Infections and bacterial resistance
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Pulmonary Hypertension Research and Treatments
- Aquaculture disease management and microbiota
- Cassava research and cyanide
- Yersinia bacterium, plague, ectoparasites research
- Microscopic Colitis
- Tuberculosis Research and Epidemiology
- Bacterial Genetics and Biotechnology
Ministry of Health and Welfare
2019-2025
Shenzhen University
2025
CS Diagnostics
2024
Development Center for Biotechnology
2019-2024
National Chung Hsing University
2021
Taichung Hospital
2017-2021
National Cheng Kung University
2021
Korea National Tuberculosis Association
1992
Antimicrobial resistance was investigated in 2,341 nontyphoidal Salmonella (NTS) isolates recovered from humans Taiwan 2017 to 2018 using antimicrobial susceptibility testing. Azithromycin determinants were detected 175 selected PCR and confirmed 81 whole-genome sequencing. Multidrug found 47.3% of total 96.2% enterica serovar Anatum 81.7% S. Typhimurium isolates. Resistance the conventional first-line drugs (ampicillin, chloramphenicol, cotrimoxazole), cefotaxime ceftazidime, ciprofloxacin...
Bacterial genotyping through whole-genome sequencing plays a crucial role in disease surveillance and outbreak investigations public health laboratories. This study assessed the effectiveness of Oxford Nanopore Technologies (ONT)
ABSTRACT We detected the colistin resistance gene mcr-1 in four Salmonella serovars isolated from humans and animals with diarrhea. The was carried on different plasmids. One -carrying conjugative plasmid, a variant of pHNSHP45, disseminated among isolates recovered humans, pigs, chickens.
Campylobacter coli and Jejuni are highly resistant to most therapeutic antimicrobials in Taiwan; rapid diagnostics of resistance bacterial isolates is crucial for the treatment campylobacteriosis. We characterized 219 (40 C. 179 jejuni ) recovered from humans 2016 2019 using whole-genome sequencing investigate genetic diversity among determinants associated with antimicrobial resistance.
The first imported case of XDR typhoid fever in Taiwan contracted with a bacterial strain, which was most closely related to the blaCTX-M-15-carrying strains linked Pakistan. Meropenem, combination an antimicrobial intracellular activity against Salmonella, should be used for treatment fever.
, a zoonotic pathogen, significantly impacts global human health. Understanding its serotype distribution and antimicrobial resistance is crucial for effective control measures medical interventions.
Pulmonary hypertension (PH) is a devastating disease marked by elevated pulmonary artery pressure, resulting in right ventricular (RV) failure and mortality. Despite the identification of several dysregulated genes PH, involvement circular RNAs (circRNAs), subset long noncoding RNAs, remains largely unknown. In this study, high-throughput RNA sequencing was performed to analyze genome-wide expression patterns circRNAs arteries from three models PH rats induced hypoxia (Hyp),...
ABSTRACT Since 2015, Taiwan has experienced a notable rise in domestic shigellosis cases, particularly among young adult males. In this study, we aimed to investigate epidemiological trend through demographic analysis and genomic characterization of bacterial isolates. We analyzed data on cases from 2003–2014 2015–2022. For 2015–2022, conducted analyses Shigella isolates using pulsed-field gel electrophoresis (PFGE) whole-genome sequencing (WGS). Antimicrobial resistance plasmid profiles...
Background Salmonella enterica serovar Paratyphi A has emerged as a significant global health concern due to the progressive development of antimicrobial resistance and its broader geographic distribution. In Taiwan, paratyphoid fever was historically rare predominantly associated with imported cases. Since 2022, however, marked increase in domestically acquired infections been observed, prompting investigations into their origin likely route introduction. Methods We analyzed surveillance...
In 2011, a Salmonella enterica serovar Anatum clone emerged in Taiwan. During 2016-2017, infections increased dramatically, strongly associated with emergence and spread of multidrug-resistant strains plasmid carrying 11 resistance genes, including blaDHA-1. Because these resistant infect humans food animals, control measures are urgently needed.
Carbapenem resistance arising from the loss of porins is commonly observed in extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase-producing strains certain Enterobacteriaceae genera, including Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa. However, this mechanism rarely reported Salmonella genus. To address knowledge gap, our study offers genetic evidence demonstrating that two specific (OmpC_378 OmpD) crucial for development carbapenem ESBL strains. Furthermore,...
Salmonella enterica serovar Goldcoast infection was rare in Taiwan; it not detected routine surveillance from 2004 to 2013. This first identified 2014, but the frequency of remained low until 2017. From 2014 2016, all one isolate pan-susceptible. S infections abruptly increased 2018, and isolates were multidrug-resistant (MDR). All MDR harbored an IncHI2 plasmid, majority carried 14 antimicrobial resistance genes, aac(3)-IId, aadA22, aph(3')-Ia, aph(6)-Id, blaTEM-1B, blaCTX-M-55, lnu(F),...
Epidemic spread of OXA-48-producing Klebsiella pneumoniae, mainly mediated by the transmission a blaOXA-48-carrying plasmid, has threatened global health during last decade. Since its introduction to Taiwan in 2013, OXA-48 become second most common carbapenemase. We described and evolution an OXA-producing K. pneumoniae clone single hospital.Twenty-two were isolated between October 2013 December 2015. Comparative genomic analysis was performed based on WGS data generated with Illumina MinION...
A CTX-M-65‒producing Salmonella enterica serovar Infantis clone, probably originating in Latin America and initially reported the United States, has emerged Taiwan. Chicken meat is most likely primary carrier. Four of 9 drug resistance genes have integrated into chromosome: blaCTX-M-65, tet(A), sul1, aadA1.
We identified an
We investigated the temporal trends of Salmonella enterica serovar Typhimurium (S. Typhimurium) clones in Taiwan from 2004 to 2019, focusing on antimicrobial resistance (AMR), genetic determinants, and plasmid types. isolates were characterized using pulsed-field gel electrophoresis (PFGE), whole-genome sequencing, susceptibility testing. Clones defined PFGE clustering hierarchical cgMLST (HierCC) assignments. Seven major S. clones, HC100_2, 13, 41, 305, 310, 501, 46261, accounted for 97.6%...
Cholera, a rapidly dehydrating diarrheal disease caused by toxigenic Vibrio cholerae, is leading cause of morbidity and mortality in some regions the world. Core genome multilocus sequence typing (cgMLST) promising approach generating genetic fingerprints from whole-genome sequencing (WGS) data for strain comparison among laboratories. We constructed V. cholerae core gene allele database using an in-house developed computational pipeline, with cgMLST profiles converted genomic sequences...
Abstract We investigated the epidemiology of cholera in Taiwan during 2002–2018. Vibrio cholerae sequence type (ST) 75 clone emerged 2009 and has since become more prevalent than ST69 from a previous pandemic. Closely related ST75 strains have 4 countries may now be widespread Asia.
In investigating the epidemiological trends of Salmonella enterica serovar Goldcoast, we previously identified several closely related strains with different MICs to azithromycin and quinolones. Genome sequencing comparison two very similar multidrug-resistant (MDR) strains, R18.0877 R18.1656, has led identification an extra plasmid-borne ramA gene, ramAp, on large IncHI2 plasmid carried by R18.0877. The ramAp gene is located in a 953-bp region plasmid, which identical that Klebsiella...
N. meningitidis can cause life-threatening invasive meningococcal disease (IMD), including meningitis and sepsis, resulting in a high CFR long-term sequelae survivors. Here, we report the demographic features of IMD Taiwan over 28-year period (1993 to 2020) genetic characteristics isolates recovered from patients with an 18-year (2003 2020).
ABSTRACT Bacterial genotyping through whole-genome sequencing plays a crucial role in disease surveillance and outbreak investigations public health laboratories. This study assessed the effectiveness of Nanopore Oxford Technologies (ONT) Listeria monocytogenes Salmonella enterica serovar Enteritidis. Our results indicated that ONT sequences, generated with R10.4.1 flow cell basecalled using Dorado 0.5.0 Super Accurate 4.3 model, exhibited comparable accuracy to Illumina effectively...