A5027438376- Protein Structure and Dynamics
- Pneumonia and Respiratory Infections
- Antifungal resistance and susceptibility
- Bacterial Infections and Vaccines
- Glycosylation and Glycoproteins Research
- Fungal Infections and Studies
- Enzyme Structure and Function
- Viral gastroenteritis research and epidemiology
- ATP Synthase and ATPases Research
- Reproductive tract infections research
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Lipid Membrane Structure and Behavior
- Hepatitis Viruses Studies and Epidemiology
- Carbohydrate Chemistry and Synthesis
- Proteoglycans and glycosaminoglycans research
- Molecular spectroscopy and chirality
- Photosynthetic Processes and Mechanisms
- Immune Response and Inflammation
- Physiological and biochemical adaptations
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Influenza Virus Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
NovaDigm Therapeutics (United States)
2012-2021
Gwynedd Mercy University
2018-2019
United States Military Academy
1992-2014
Grand Forks Air Force Base
2013
Merck & Co., Inc., Rahway, NJ, USA (United States)
1992-2005
Merck (Japan)
1997
University of North Carolina at Chapel Hill
1988-1990
Oregon State University
1982-1987
Oregon Department of Fish and Wildlife
1987
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInformation content in the circular dichroism of proteinsJohn P. Hennessey, Jr. and W. Curtis Johnson, Jr.Cite this: Biochemistry 1981, 20, 5, 1085–1094Publication Date (Print):March 1, 1981Publication History Published online1 May 2002Published inissue 1 March 1981https://pubs.acs.org/doi/10.1021/bi00508a007https://doi.org/10.1021/bi00508a007research-articleACS PublicationsRequest reuse permissionsArticle Views1742Altmetric-Citations477LEARN ABOUT...
Recurrent vulvovaginal candidiasis (RVVC) is a problematic form of mucosal Candida infection, characterized by repeated episodes per year. albicans the most common cause RVVC. Currently, there are no immunotherapeutic treatments for RVVC.This exploratory randomized, double-blind, placebo-controlled trial evaluated an vaccine (NDV-3A) containing recombinant C. adhesin/invasin protein prevention RVVC.The study in 188 women with RVVC (n = 178 evaluable) showed that 1 intramuscular dose NDV-3A...
Increasing rates of life-threatening infections and decreasing susceptibility to antibiotics urge development an effective vaccine targeting Staphylococcus aureus. This study evaluated the efficacy immunologic mechanisms a containing recombinant glycoprotein antigen (NDV-3) in mouse skin structure infection (SSSI) due methicillin-resistant S. aureus (MRSA). Compared with adjuvant alone, NDV-3 reduced abscess progression, severity, MRSA density skin, as well hematogenous dissemination kidney....
Staphylococcus aureus is the leading cause of skin and structure infections (SSSI) in humans. Moreover, high frequency recurring SSSI due to S. aureus, particularly methicillin-resistant (MRSA) strains, suggests that infection induces suboptimal anamnestic defenses. The present study addresses hypothesis interleukin-17A (IL-17A) IL-22 play distinct roles immunity cutaneous invasive MRSA a mouse model SSSI. Mice were treated with specific neutralizing antibodies against IL-17A and/or infected...
Glycoconjugate vaccines are a critical component of the medical arsenal against infectious diseases. This established field continues, however, to experience failures in clinic. The lack fundamental understanding factors controlling clinical efficacy glycoconjugate is discussed while key parameters demanding focused and collaborative research identified.
A Phase 1b/2a clinical trial of NDV-3A vaccine containing a Candida albicans recombinant Als3 protein formulated with alum protected women <40 years old from recurrent vulvovaginal candidiasis (RVVC). We investigated the potential use anti-Als3p sera as surrogate marker efficacy. Pre- and post-vaccination subjects who experienced recurrence (R) vs. those were recurrence-free [non-recurrent (NR)] evaluated. Anti-Als3p antisera obtained evaluated for (1) titer subclass profile (2) their...
Reconstituted proteoliposomes containing Neurospora plasma membrane H+-ATPase molecules oriented predominantly with their cytoplasmic portion facing outward have been used to determine the location of NH2 and COOH termini relative lipid bilayer. Treatment trypsin in presence ligands Mg2+, ATP, vanadate produces approximately 97-, 95-, 88-kDa truncated forms similar those already known result from cleavage at Lys24, Lys36, Arg73 NH2-terminal end molecule. These results establish that...
Abstract CD measurements were made for calmodulin and its calcium (Ca 2+ ) complexes at different ionic strengths Ca concentrations. Calmodulin an strength of 0.00 M in the absence exists as α‐helical protein with a negligible amount β‐sheet. An increase strength, whether or not is present, increases α‐helix expense “other” (coil) structure. The changes β‐sheet β‐turns are insignificant. Binding low occurs stages least one folding intermediate before attaining final stable state. 0.165...
Abstract Reconstituted proteoliposomes containing functional Neurospora crassa plasma membrane H(+)-ATPase molecules oriented predominantly with their cytoplasmic surface exposed were treated trypsin and then subjected to Sepharose CL-6B column chromatography remove the liberated peptides. The peptides remaining associated liposomes separated from phospholipid by Sephadex LH-60 analyzed sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Six approximate molecular masses of 7, 7.5, 8,...
crassa plasma membrane H+-ATPase was described (Chadwick, C .C., Goormaghtigh, E., and Scarborough, G . A. (1987) Arch.Biochem.Biophys.252,348-356).To facilitate physical studies of the hexamers, iso- lation procedure has been improved, resulting in a structurally functionally stable hexamer preparation that contains only 6 to 10% non-ATPase protein, approximately 12 mol enzyme-bound lysophosphatidylcholine/mol monomer, little or no residual phospholipid.Importantly, when activated by...
The transmembrane topography of the Neurospora crassa plasma membrane H(+)-ATPase has been investigated using purified, reconstituted components and direct protein chemical techniques. Reconstituted proteoliposomes containing molecules oriented predominantly with their cytoplasmic surface facing outward were treated trypsin to liberate peptides present on as recently described (Hennessey, J.P., Jr., Scarborough, G. (1990) J. Biol. Chem. 265, 532-537. released then separated from by gel...